This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.

96-well plate Application Notes B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Serum Vitamin B3 Vitamins

This application note describes the extraction of nine phthalate metabolites from human urine using ISOLUTE® ENV+ solid phase extraction columns.

Application Notes Biomarker Clinical Column English LC-MS/MS Mono(2-ethyl-5-carboxypentyl) phthalate (MECPP) Mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono(2-ethylhexyl) phthalate (MEHP) Monobenzyl phthalate (MBzP) Monobutyl phthalate (MBP) Monoethyl phthalate (MEP) Monohexyl phthalate (MHxP) Monoisononyl phthalate (MiNP) Monomethyl phthalate (MMP) Phthalate Metabolites Phthalates Urine

This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.

Alcohol metabolite Application Notes Clinical Column Ethyl Sulfate (EtS) Ethyl glucuronide (EtG) Forensic LC-MS/MS Urine

The development of a novel high-pressure liquid chromatography/ triple quad mass spectrometry method allows for the efficient and accurate identification of 12 synthetic cannabinoids in whole blood by LC-MS/MS. Samples are fortified with internal standard, buffered, and extracted using Supported Liquid Extraction (SLE).

Application Notes Clinical Column Forensic LC-MS/MS Synthetic Cannabinoids (SPICE)

Aflatoxins, a mold largely produced by Aspergillus flavus and Aspergillus parasiticus1 are commonly tested mycotoxins found naturally in a wide range of agriculture crops and food products. Due to their harmful effects on human health, animal health, and global trade, aflatoxins are regulated in most countries and have established global limits in a wide variety of matrices.

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Column English Food Food HPLC Mycotoxin

The compound 4-methylimidazole (4-MEI) is formed as a byproduct in some foods and beverages. Caramel coloring Type III and Type IV in beverages is one of the ingredients which may contain 4-MEI. Products that potentially contain 4-MEI include certain colas, beers, soy sauces, breads, coffee, ammoniated livestock feed and other products.

4-Methylimidazole (4-MEI) Application Notes Food Food Food Additives Food Colours LC-MS/MS SmartPrep

The DryDisk® Separation Membrane was designed to replace conventional sodium sulfate in the drying of sample extracts. The DryDisk® is a hydrophobic membrane, consisting of a GORETEX ® process filtration media based on polytetrafluroethylene (PTFE). The hydrophobic property of the membrane allows the extracting solvent to pass through it, while retaining any water in the sample extract on the membrane’s surface.

Application Notes English Environmental Organic Compounds Water

Mycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply. Several mycotoxins are naturally produced and monitored; however, aflatoxins, produced mainly by the Aspergillus species, are considered to be the most important mycotoxin group in the world’s food supply to monitor.

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes English Food Food Mycotoxin SmartPrep

This application note describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetyl codeine 96-well plate Acidic Alprazolam Amphetamine Application Notes Barbiturates Basic Benzodiazepines Benzoylecgonine Buprenorphine Butalbital Clinical Clonazepam Codeine Dextromethorphan Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydrolysed urine Hydromorphone LC-MS/MS MDEA Naltrexone Neutral Nitrazepam Norbuprenorphine Norcodeine Norfentanyl Normeperidine Opiates Oxazepam Oxycodone Oxymorphone Pain Management Pentazocine Pentobarbital Phenobarbital Propoxyphene Secobarbital THC metabolites Temazepam Urine Δ9-tetrahydrocannabinol (THC)

La cromatografía flash en fase normal y fase reversa facilita el aislamiento de compuestos polares presentes en extractos de productos naturales. La combinación de avanzadas funciones de detección tales como barrido espectral, y evaporativo de luz dispersa (ELSD) incrementa la efectividad de la cromatografía flash en procesos de purificación de productos naturales.

Application Notes Spanish

Effective removal of residual water from organic solvents is critical to achieve optimal recoveries for water soluble compounds. Sodium sulfate has been the standard technique for removing residual water from solvent extracts for many years; however, it has several major drawbacks. Water soluble analytes in the residual water phase can become adsorbed in the sodium sulfate, leading to lower recoveries.

Application Notes Disk English Environmental GC-MS Organic Compounds Water

This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates. EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.

6-acetyl codeine 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amitriptyline Amphetamine Amphetamines Analgesics Application Notes Aripiprazole Atomoxetine Barbiturates Benzodiazepines Benzoylecgonine Buprenorphine Bupropion Buspirone Butalbital Carbamate hypnotic Carbamate muscle relaxant Carbamates Carbazepine Carisoprodol Chlordiazepoxide Chlorpheniramine Chlorpromazine Clinical Clomipramine Clonazepam Clonidine Clozapine Cocaethylene Cocaine Cocaines Codeine Cyclobenzaprine Desalkylflurazepam Designer stimulants Dextromethorphan Diazepam Dihydrocodeine (DHC) Drugs of Abuse Duloxetine EDDP English Ethyl Sulfate (EtS) Extrahera Fentanyl Fluoxetine Forensic Gabapentin Haloperidol Hydrocodone Hydrolysed urine Hydromorphone Hydroxyalprazolam Hydroxybupropion Hydroxymidazolam Hydroxytriazolam Imipramine Ketamine LC-MS/MS Lamotrigine Levetiracetam Lidocaine Lorazepam MDA MDEA MDMA Meperidine Meprobamate Methadone Methamphetamine Methaqualone Methcathinone Midazolam Morphine N-des-tapentadol N-desmethylclomipramine N-desmethyltapentadol Naloxone Norbuprenorphine Nordiazepam Norfentanyl Norhydrocodone Norketamine Normeperidine Noroxymorphone Norpropoxyphene Nortryptiline Opiates Opioid Opioid agonist Oxazepam Oxcarbazepine Oxycodone Oxymorphone Pain Management Pentobarbital Pharmaceuticals Phencyclidine (PCP) Phenobarbital Pregabalin Quetiapine Ritalinic acid Secobarbital Stimulants THC-COOH Tapentadol Temazepam Topiramate Tramadol Triazolam Trimipramine Urine Venlafaxine Zolpidem Zolpidem-phenyl-4-COOH mCPP z-drugs

This application note evaluates the effectiveness of bead mill homogenization on the Biotage® Lysera for multiple pesticide extraction using the QuEChERS AOAC 2007.01 method.

Application Notes Food Lysera Pesticides QuEChERs Vegetables

This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.

2,4,5-T 2,4-D 2,4-DB Acid herbicides Application Notes Asulam Benazolin Bentazone Bromoxynil Dicamba Dichlorprop Drinking water English Environmental Ioxynil MCPA MCPB Mecoprop On-line SPE On-line SPE-LC-MS/MS Pentachlorophenol Quinmerac Surface water Triclopyr Water

Pesticides, including insecticides, herbicides and fungicides are used extensively to increase agricultural yields. The total world usage of pesticides in 2007 was approximately 5.2 billion pounds with the US consuming approximately 22% of the total.1 The wide use of pesticides yields concern that drinking water sources will become contaminated, exposing the population to hazardous substances that may cause cancer.

Application Notes Disk Drinking water EPA 508.1 Analytes English Environmental GC-MS Pesticides

This application note demonstrates that the Biotage(R) Horizon 4790 using Atlantic C18 disks can effectively extract hormone compounds from 1-L water samples in approx 40 mins.

11-ketotestosterone 17-alpha-estradiol 17-alpha-ethynylestradiol 17-beta-estradiol 4790 Androgens Androsterone Application Notes Diethylstilbestrol Dihydrotestosterone (DHT) Disk Endocrine disrupter English Environmental Epitestosterone Estradiol Estriol Estrogens Estrone GC-MS Hormone Hormones Mestranol Norethindrone Progesterone Steroid Steroid Hormones Steroid metabolite Steroids Surface water Testosterone Water

Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.

Application Notes Column Drinking water English Environmental LC-MS/MS Polyfluorinated acids

This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water, wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.

4790 Acetaminophen Albuterol Albuterol Application Notes Atenolol Caffeine Carbamazepine Cotinine DEET Dehydronifedipine Diclofenac Diltiazem Diphenhydramine hydrochloride Disk Drinking water English Environmental Gemfibrozil Ground water Ibuprofen LC-MS TOF Lamotrigine Metoprolol Naproxen Pharmaceuticals Sulfadimethoxine Sulfamethoxazole Triclocarban Trimethoprim Waste water

This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.

Application Notes Atenolol Atorvastatin Azithomycin Carbamazepine Ciprofloxacin Clarithromycin Diclofenac English Environmental Erythromycin Fluoxetine On-line SPE On-line SPE-LC-MS/MS Pharmaceuticals Prednisolone Ranitidine Sertraline Tolytriazole Water