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AFFINILUTE™ MIP ColumnsAlstra™ RemoteAtlantic® SPE DisksBiotage Service & Support - EuropeBiotage ZIP®Biotage ZIP® SphereBiotage® Flash 400 SystemBiotage® ACT Plate AdapterBiotage® AdvancerBiotage® AtlantisBiotage® ELSD-1080Biotage® ELSD-A120Biotage® Endeavor™Biotage® Extrahera™Biotage® Flash 150 SystemBiotage® Flash 400 SystemBiotage® Flash 75 SystemBiotage® Flash 75/150Biotage® Horizon 3100Biotage® Horizon 4790Biotage® Horizon 5000Biotage® Horizon SmartPrepBiotage® Horizon SPE-ZBiotage® Initiator ClassicBiotage® Initiator Peptide WorkstationBiotage® Initiator+Biotage® Initiator+ Alstra™Biotage® Initiator+ SP WaveBiotage® LyseraBiotage® MP-BorohydrideBiotage® MP-CarbonateBiotage® MP-CyanoborohydrideBiotage® MP-IsocyanateBiotage® MP-TMTBiotage® MP-TriacetoxyborohydrideBiotage® MP-TrisamineBiotage® MP-TsOHBiotage® PRESSURE+Biotage® PS-BenzaldehydeBiotage® PS-CarbodiimideBiotage® PS-HOBt(HL)Biotage® PS-IsocyanateBiotage® PS-PPh3-PdBiotage® PS-TBDBiotage® PS-TEMPOBiotage® PS-TriphenylphosphineBiotage® PS-TsNHNH2Biotage® Resolux™Biotage® Robot Eight & Robot SixtyBiotage® SelektBiotage® SfärBiotage® SNAP BioBiotage® SNAP KP-C18-HSBiotage® SNAP KP-NHBiotage® SNAP KP-SilBiotage® SNAP UltraBiotage® SNAP Ultra C18Biotage® Solvent Recovery OGBiotage® Solvent Recovery SVOCBiotage® SPBiotage® SPE DryBiotage® Syro Wave™Biotage® V-10 EvaporatorBiotage® V-10 TouchBiotage® VacMaster™ 10 and 20Biotage® VacMaster™ 96Cartridge Processing StationsDry Load VesselsDryDisk® Disks & BarrelsDryVap®Empty Cartridges, Frits, Caps and StoppersEVOLUTE® ABNEVOLUTE® AXEVOLUTE® CXEVOLUTE® EXPRESSEVOLUTE® EXPRESS ABNEVOLUTE® EXPRESS ABN On-line SPE CartridgesEVOLUTE® EXPRESS AXEVOLUTE® EXPRESS CXEVOLUTE® EXPRESS WAXEVOLUTE® EXPRESS WCXEVOLUTE® HYDROEVOLUTE® WAXEVOLUTE® WCXFlash 75/150/400 CartridgesFlashMaster Personal PlusGravity RackHMPB-ChemMatrix®Isolera™ DaltonIsolera™ Dalton 2000Isolera™ PrimeIsolera™ Spektra Systems with ACI™ and AssistISOLUTE® SCX-2ISOLUTE® 101ISOLUTE® AccessoriesISOLUTE® AL-A, AL-N & AL-BISOLUTE® C18ISOLUTE® C18(EC)ISOLUTE® C2ISOLUTE® C2(EC)ISOLUTE® C4ISOLUTE® C8ISOLUTE® C8(EC)ISOLUTE® CBAISOLUTE® CNISOLUTE® DIOLISOLUTE® ENV+ISOLUTE® ENV+ On-line SPE CartridgesISOLUTE® EPHISOLUTE® FILTER+ High Performance Filtration PlatesISOLUTE® FLISOLUTE® HAXISOLUTE® HCXISOLUTE® HCX-3ISOLUTE® HCX-5ISOLUTE® HCX-QISOLUTE® HM-N ColumnsISOLUTE® HYDRO DME+ Dual Mode Extraction ProductsISOLUTE® Layered SPE ColumnsISOLUTE® MFC18ISOLUTE® MultimodeISOLUTE® MycoISOLUTE® Na2SO4/FLISOLUTE® NH2ISOLUTE® O&GISOLUTE® PAHISOLUTE® PE-AXISOLUTE® PHISOLUTE® PLD+ Protein and Phospholipid Removal ProductsISOLUTE® PPT+ Protein Precipitation PlatesISOLUTE® PSAISOLUTE® QuEChERSISOLUTE® SAXISOLUTE® SAX/PSAISOLUTE® SCXISOLUTE® SCX-2ISOLUTE® SCX-3ISOLUTE® SIISOLUTE® Si-ThiolISOLUTE® Si-TMTISOLUTE® Si-TrisamineISOLUTE® SLE+ Supported Liquid Extraction ProductsISOLUTE® Sodium Sulfate Drying CartridgeISOLUTE® TPHKILOPREP Systems and ConsumablesLarge Scale Flash AccessoriesLarge Scale Flash CartridgesMetal Scavenging Screening KitsMicrowave Reaction VialsMIPs - Molecularly Imprinted PolymersPacific® Oil & Grease DisksPAL-ChemMatrix®Peptide Association LinksPhase SeparatorsRacks, Bottles & Leak DetectorsRapidTrace®+RENSA® Bulk ResinsResin Development ServiceRink Amide-ChemMatrix®SAMSamplet® CartridgesSAPService Agreements - EuropeService Agreements - North and Latin AmericaSpeedVap®Stop-cocks, Needles, Scoops and AdaptersSyro Heating BlocksSyro ISyro IITLC PlatesTrityl-ChemMatrix®TurboVap® 96TurboVap® Classic 500TurboVap® Classic IITurboVap® Classic LVTurboVap® EHTurboVap® IITurboVap® LVTurboVap® P+UV Monitor Biotage® Flash 150/400VacMaster™ AccessoriesValves, Adapters, Holders & CapsWang-ChemMatrix®Work-up CartridgesXcelVap®

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Format

4790 48-well plate 5000 96-well plate Bead Ruptor Column Disk Extrahera Lysera On-line SPE QuEChERs RapidTrace TECAN

Analytical Technique

GC-FID GC-FPD GC-MS GC-PID GC/ECD GC/MS/MS Gravimetric HPLC HPLC-DAD LC-MS TOF LC-MS/MS LDTD-MS/MS On-line SPE-LC-MS/MS

Analyte Type

Acid herbicides Acidic Acidic catecholamine metabolites Acifluorfen Adipate esters Alcohol metabolite Aliphatic hydrocarbons Alkaloids Aminoglycoside antibiotics Amphetamines Anabolic steroids Anaesthetic Analgesics Antibiotics Anticoagulant Anticonvulsant Antiepileptics Antimicrobial Antipsychotics Aromatic hydrocarbons B Vitamins BDE-153 BDE-99 Barbiturates Base-neutral herbicides Basic Benzodiazepines Beta blockers Biomarker Brominated Flame Retardants (BFRs) Bronchodilator Cannabinoid Carbamate hypnotic Carbamate muscle relaxant Carbamates Catecholamines Cathinones Chlorinated acids Cocaines Corrosion inhibitors Corticosteroids Designer stimulants Diarrhetic Shellfish Poisoning (DSP) Toxins Dinoseb Diuretics Dried chili Drugs of Abuse EN16691 Analytes EN16693:2015 analytes EPA 1657 analytes EPA 1664 A analytes EPA 1664 B analytes EPA 506 Analytes EPA 548.1 Analytes EPA 549.2 Analytes EPA 608 analytes EPA 625.1 Analytes EPA 8015 Analytes Endocrine disrupter Environmental contaminants Estrogens Explosives Fat Soluble Vitamins Fluoroquinolones Food contaminant Hallucinogen Haloacetic Acids Hexabromobiphenyl Hexane Extractable Material Imidazoline corrosion inhibitor Imidazolines Immunosuppressant Legal highs Lipids Marine Toxins Metanephrines Mycotoxin NBOMes NSAIDs Neutral Nicotine metabolites Nitroimidazoles Opiates Opioid Opioid agonist Organic Compounds Organochlorine pesticides (OCP) Organophosphate Pesticide Over the counter pharmaceuticals PAHs PBDEs PCBs Pain Management Paralytic Shellfish Poisoning (PSP) Toxins Peptides Persistant organic pollutants (POP) Perylene-d12 Pesticides Pharmaceuticals Phenethylamines Phenols Phthalate Metabolites Phthalates Phthalic acid esters Plasticisers Polyaromatic Hydrocarbons (PAH) Polyfluorinated acids Preservative Quaternary Amines RDX SERM (selective estrogen receptor modulator) Semi-Volatile Organics Spice Steroid Hormones Steroid metabolite Stimulants Sympathomimetic amine Synthetic Cannabinoids (SPICE) THC metabolites Thyroid Hormone Tobacco Specific Nitrosamines (TSNAs) Triazine Herbicides Triazines Tricyclic Antidepressant Triphenylmethane Dyes Veterinary drugs Vitamin D Vitamins Water Soluble Vitamins tetryl z-drugs β-agonists β-blockers

Matrix

Animal feed Apple juice (clear) Aqueous BSA Barley Biological fluids Bovine kidney Brain Brazil nut Carbonated soft drink (soda) Chicken meat Coffee Dried chili Drinking water Eggs Enzyme powder Fish Food Grain Ground water Groundnut (Peanut) Hair Hemolysed whole blood Honey Hydrolysed urine Infant food (cereal) Infant formula (milk) Liver Maize Meat Meconium Milk Mouse plasma Nuts Olive Oil Oral fluid Pig's Blood Plasma Post-Mortem fluids Post-Mortem tissue Potato chips (crisps) Prawns Saliva Salmon Sediment Serum Shellfish Shrimps Sludge cake Soil Spice Stomach contents Sun Lotion Sunscreen Surface water Synthetic urine Urine Urine (horse) Vegetables Waste water Water Wheat Wheat flour Whole blood Wine (red)

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2 results

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Extraction of a Comprehensive Steroid Panel from Human Serum Using EVOLUTE® EXPRESS ABN Prior to LC/MS-MS Analysis

Part No: AN891 Issued year: 2018 File size: 2.35mb File type: pdf

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite

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Extraction of a Comprehensive Steroid Panel from Human Serum Using ISOLUTE® SLE+ Prior to LC/MS-MS Analysis

Part No: AN890 Issued year: 2018 File size: 1.73mb File type: pdf

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

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