Extraction of Benzodiazepines From Human Urine Using ISOLUTE® SLE+ in Column Format Prior to GC-MS analysis
Part No: AN756
Issued year: 2012
File size: 2.06mb
File type: pdf
Benzodiazepines are psychoactive drugs used to treat various conditions including anxiety, insomnia, seizures and alcohol withdrawal. Long-term use can cause physical dependence and withdrawal upon cessation of use, due to this their application as medication has to be monitored and measured. This application note details an optimized extraction protocol for the use of ISOLUTE SLE+ supported liquid 1 mL sample volume columns to extract a range of benzodiazepines from human urine. This simplified and efficient extraction method has analyte recoveries from 90% with LOQs as low as 40 ng/mL. The application has also been performed with halogenated and non-halogenated extraction solvents in the interests of providing for green chemistry.
Drugs of abuse, DOA, Benzodiazepines, SLE, SLE+, Supported Liquid Extraction, Green Chemistry,
2-Hydroxyethylflurazepam
4-Hydroxynordiazepam
7-Aminoclonazepam
7-Aminoflunitrazepam
7-Aminonitrazepam
Alprazolam
Application Notes
Benzodiazepines
Bromazepam
Clonazepam
Demethylflunitrazepam
Desalkylflurazepam
Diazepam
Estazolam
Flunitrazepam
Flurazepam
Forensic
GC-MS
Hydroxyalprazolam
Hydroxymidazolam
Hydroxytriazolam
Midazolam
Nitrazepam
Nordiazepam
Oxazepam
Prazepam
Temazepam
Triazolam
Urine