In postmortem cases, where drugs or pesticides have been used for their poisonous properties, traditional matrices such as urine and whole blood may be inappropriate for qualitative and quantitative analysis. As the site of metabolism for most drugs and toxins, the liver may provide more insight to cause of death than other bodily fluids. This poster describes the use of ISOLUTE SLE+ supported liquid extraction columns to extract a range of drug and pesticide classes form homogenised liver using a simple, streamlined workflow. SOFT 2016

Alprazolam Amphetamine Atrazine Bead Ruptor Bendiocarb Biphenthrin Butabarbital Chlorothalonil Clinical Clonazepam Cocaine Column Diazepam Drugs of Abuse English Estazolam Forensic GC-MS Ketamine Liver MDMA Malathion Methadone Methamphetamine Midazolam Nitrazepam Pesticides Phenobarbital Posters Propanil Secobarbital THC Triazolam

This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and consumable costs. SOFT 2015 ASMS 2016 TIAFT 2016

2-Hydroxyethylflurazepam 2C-B 5-APB 6-APB 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amobarbital Amphetamine Anhydroecgonine Methyl Ester AEME BZP Benzoylecgonine Bromazepam Butabarbital Butalbarbital Butylone Clinical Clonazepam Cocaethylene Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse Ecgonine Methyl Ester EME English Estazolam Ethylone Flunitrazepam Flurazepam Forensic GC-MS Hexobarbital Hydrocodone Hydromorphone Lorazepam MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Midazolam Morphine Naphyrone Nitrazepam Nordiazepam Oxazepam Oxycodone Oxymorphone Pentobarbital Phenobarbital Posters Secobarbital TFMPP Temazepam Triazolam Whole blood mCPP pMMA α-OH Triazolam α-OH alprazolam

This method describes the use of ISOLUTE SLE+ supported liquid extraction 1 mL sample volume columns to extract a range of barbiturates from human urine. The analysis of these analytes was carried out by GC-MS. This simplified and efficient extraction method has significant analyte recoveries ranging from 103-108% with LOQs of 10 ng/mL and RSDs <10%. Barbs, Barbiturates, SLE, SLE+, ISOLUTE, GCMS, DoA, Forensic, Work Place Drug Testing, Supported Liquid Extraction, GC

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital GC-MS Phenobarbital Secobarbital Urine

The objective was to develop a GC-MS assay for the determination of barbiturates from urine matrix using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods. Barbiturates, SLE, Supported Liquid Extraction, Forensic, SOFT, Urine,

Barbiturates Butabarbital Butalbarbital GC-MS Pentobarbital Posters Secobarbital Urine

This application note describes the extraction of barbiturates from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit(Orasure Technologies), prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 7% for all analytes.

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Column Drugs of Abuse Forensic Hexobarbital LC-MS/MS Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction of barbiturates, using ISOLUTE SLE+ supported liquid extraction columns, from oral fluid matrix collected using the NeoSal collection device, prior to GC/MS analysis.

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Column Drugs of Abuse English Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction of Butalbarbital, Butabarbital, Amobarbital, Pentobarbital,Secobarbital, Hexobarbital and Phenobarbital from oral fluid matrix collected using the Oral-Eze® collection devices, prior to GC/MS analysis.

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Column English Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction of barbiturates from oral fluid matrix collected using Quantisal™ collection devices (Immunalysis), prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 88% with RSDs lower than 6% for all analytes.

96-well plate Amobarbital Application Notes Butabarbital Butalbarbital Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This poster describes the extraction of barbiturates from oral fluid collected using common collection devices using ISOLUTE SLE+ Supported Liquid Extraction columns, optimised for GC-MS analysis. SOFT 2014

Amobarbital Barbiturates Butabarbital Butalbarbital Column English Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Posters Secobarbital

The following method was developed for the extraction of barbiturate drugs from urine, including: butabarbital, amobarbital, cyclobarbital and phenobarbital. Typical recoveries for these drugs are > 85%. ISOLUTE, BIOTAGE, HCX, DOA, Nervous System

Amobarbital Application Notes Barbiturates Butabarbital Clinical Cyclobarbital GC-MS Phenobarbital Urine

This application note describes the extraction of barbiturate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, benzodiazepines, cocaine and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Clinical Column English GC-MS Hexobarbital Pentobarbital Phenobarbital Secobarbital Whole blood

This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns. A protocol has been developed that allows the simultaneous extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC. In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.

Alprazolam Amphetamine Application Notes Atrazine Bead Ruptor Bendiocarb Biphenthrin Butabarbital Chlorothalonil Clinical Clonazepam Cocaine Cocaines Column Diazepam Drugs of Abuse English Estazolam Forensic GC-MS Ketamine Liver MDMA Malathion Methadone Methamphetamine Midazolam Nitrazepam Pesticides Phenobarbital Propanil Secobarbital THC Triazolam

This poster describes a simple but effective method for screening a range of pesticides and drugs of abuse from liver samples. Liver tissue is homogenized using the Biotage® Lysera, and the subsequent extract is cleaned up using ISOLUTE SLE+ supported liquid extraction columns. ASMS 2017

Alprazolam Amphetamine Amphetamines Atrazine Barbiturates Bead Ruptor Bendiocarb Benzodiazepines Biphenthrin Butabarbital Chlorothalonil Clinical Clonazepam Cocaine Cocaines Column Diazepam Drugs of Abuse English Estazolam Forensic GC-MS Ketamine Liver MDMA Malathion Methadone Methamphetamine Midazolam Nitrazepam Organochlorine pesticides (OCP) Pesticides Phenobarbital Posters Propanil Secobarbital THC Triazolam