This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite Testosterone

This application note describes the extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ supported liquid extraction plates (96-well) with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% withb LOQs as low as 500 pg/mL. ISOLUTE, TESTOSTERONE, SLE, SLE+, SUPPORTED LIQUID EXTRACTION, STEROID, HORMONE, PLASMA, ANDROGEN, CLINICAL

11-deoxycortisol 17-alpha-hydroxyprogesterone 21-deoxycortisol Aldosterone Androstenedione Androsterone Application Notes Clinical Dehydroepiandrosterone (DHEA) LC-MS/MS Plasma Progesterone Steroid Hormones Testosterone

This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility. MSACL 2016

11-deoxycortisol 11-keto-androsterone 11-oxoetiocholanolone 11-β-hydroxyandrosterone 11β-hydroxyetiocholanolone 2-OH-estrone 2-methoxyestrone 3α-androstanediol 3β-androstanediol 4-OH-estrone 4-methoxyestrone 96-well plate Allo-THB Androsterone Clinical Corticosterone DHEA (dehydroepiandrosterone Dehydroepiandrosterone (DHEA) English Etiocholanolone Extrahera Hydrolysed urine Posters Steroid Hormones Steroid metabolite THB THF Testosterone α-THF