This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and consumable costs. SOFT 2015 ASMS 2016 TIAFT 2016

2-Hydroxyethylflurazepam 2C-B 5-APB 6-APB 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amobarbital Amphetamine Anhydroecgonine Methyl Ester AEME BZP Benzoylecgonine Bromazepam Butabarbital Butalbarbital Butylone Clinical Clonazepam Cocaethylene Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse Ecgonine Methyl Ester EME English Estazolam Ethylone Flunitrazepam Flurazepam Forensic GC-MS Hexobarbital Hydrocodone Hydromorphone Lorazepam MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Midazolam Morphine Naphyrone Nitrazepam Nordiazepam Oxazepam Oxycodone Oxymorphone Pentobarbital Phenobarbital Posters Secobarbital TFMPP Temazepam Triazolam Whole blood mCPP pMMA α-OH Triazolam α-OH alprazolam

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample (hydrolysed urine) volumes of either 200 μL or 1 mL. Procedures for high throughput (96-well plate) and column format are included. The simple sample preparation procedure delivers clean extracts and analyte recoveries of 82-100% with RSDs of <5% for all analytes. Sub ng/mL LOQs can be achieved.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Butylone Cocaines Column Ecgonine Methyl Ester EME Forensic Hydrolysed urine LC-MS/MS Norcocaine

This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample (urine) volumes of either 200 μL or 1 mL. Procedures for high throughput (96-well plate) and column format are included. The simple sample preparation procedure delivers clean extracts and analyte recoveries of 67-100% with RSDs of <5% for all analytes. Sub ng/mL LOQs can be achieved.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Butylone Cocaines Column Ecgonine Methyl Ester EME Forensic LC-MS/MS Norcocaine Urine

This application note describes effective and efficient protocols for extraction of cocaine and its major metabolites optimized for sample loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <7% for all analytes.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Hydrolysed urine Norcocaine

This application note describes the extraction of cocaine and selected metabolites from oral fluid matrix following the collection with the NeoSalTM device, prior to GC/MS analysis.

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME Ecgonine methyl ester English Forensic GC-MS Norcocaine Oral fluid Saliva

This application note describes effective and efficient protocols optimized for loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs of <10% for all analytes.

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME Ecgonine methyl ester English Forensic GC-MS Norcocaine Oral fluid

This application note describes effective and efficient protocols optimized for sample volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs of <10% for all analytes.

Anhydroecgonine Methyl Ester AEME Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Norcocaine Urine

This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Clinical Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Whole blood