This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application achieves high reproducible recoveries and excellent linearity (>0.99) in the range 10-200 ng/mL.

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Application Notes Clinical English Homovanillic acid (HVA) LC-MS/MS Plasma Vanillylmandelic acid (VMA)

This application note describes a supported liquid extraction protocol for the extraction of three acidic catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application note achieves high reproducible recoveries (>80%, RSD < 5%) for these analytes in plasma with linearity > 0.995 in the range 2–200 ng/mL.

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Application Notes Clinical English Homovanillic acid (HVA) LC-MS/MS Plasma Vanillylmandelic acid (VMA)

Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) are biomarkers for neuroblastoma and catecholaminesecreting tumors. This poster describes optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Parameters investigated include MRM transitions, chromatography and sample preparation protocols. MSACL EU 2017

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Catecholamines Clinical English Homovanillic acid (HVA) LC-MS/MS Plasma Posters Vanillylmandelic acid (VMA)

Catecholamine metabolites vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5 HIAA) are biomarkers for neuroblastoma and catecholamine-secreting tumors. This poster presents optimization of the method development process to maximize analyte sensitivity in the extraction and quantitation of these metabolites from plasma. Sample preparation protocols using solid phase extraction (EVOLUTE EXPRESS ABN and AX) and supported liquid extraction (ISOLUTE SLE+) are presented. MSACL NA 2018 Palm Springs, CA

5-hydroxyindoleacetic acid (5-HIAA) 96-well plate Acidic catecholamine metabolites Catecholamines Clinical English Forensic Homovanillic acid (HVA) LC-MS/MS Plasma Posters Vanillylmandelic acid (VMA)