Initiator+ Alstra Testimonial Dr Longhi

Customer Cases English

This outlines the procedure to install the one-pass kit onto a VacMaster Disk station as well as provides basic instructions in its use.

Disk English Environmental Food Manuals & User Guides Petroleum

VacMaster Disk Getting Started Guide (User Manual)

English Environmental Manuals & User Guides Petroleum

Connecting Flash cartridge to Biotage Flash systems

English Manuals & User Guides

This poster compares various sample preparation techniques (mixed-mode SPE (EVOLUTE EXPRESS CX, phospholipid depletion (ISOLUTE PLD+), supported liquid extraction (ISOLUTE SLE+) and dual mode extraction (ISOLUTE HYDRO DME+) for extraction of a panel of 98 drugs of abuse from urine. SOFT 2019

96-well plate Amphetamines Anticonvulsant Antipsychotics Barbiturates Benzodiazepines Cannabinoid Clinical English Fentanyls Forensic Hallucinogen LC-MS/MS Opiates Opioid Posters Stimulants z-drugs

This poster compares solid phase extraction (EVOLUTE EXPRESS CX and ISOLUTE HCX) with supported liquid extraction (ISOLUTE SLE+) for extraction of fentanyl analogues from whole blood samples. NEAFS 2019

4-ANPP (4-aminophenyl-1-phenethylpiperidine) 4-fluoro-isobutyryl-fentanyl 96-well plate Acryl fentanyl Alfentanil Butyryl fentanyl Carfentanil Clinical English Fentanyl Fentanyls Forensic Furanyl fentanyl Isobutryl fentanyl Methoxyacetyl fentanyl Norfentanyl Opioid Posters Sufentanil U-47700 U-51754 Valeryl fentanyl Whole blood

Corrosion costs the petroleum industry an estimated $1.3 billion in non-productive time, materials and labor annually. Imidazolines prepared from fatty acids and amines are a widely-used class of chemical corrosion inhibitor, due to excellent performance and ease of handling. However, commercial imidazolines are actually mixtures of several different chemical compounds, and the relative proportions of these species can have a large impact on both corrosion inhibition and product physical properties. The absence of gold standard analytical methods to characterize the active ingredients in imidazoline formulations limits the understanding of the chemistry of these materials. It is for this reason that a SPE-LC-MS method was developed to supplement the chemical information afforded by bulk testing / wet chemistry methods. It is anticipated that this method will have significant impact in the formulation of new corrosion inhibitors for oilfield applications as well as the quality control of finished products in manufacturing. GCC 2014

Column Corrosion inhibitors English Imidazoline corrosion inhibitor Imidazolines LC-MS TOF Petroleum Posters

This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

This poster details current sample preparation strategies for aminoglycoside antibiotics in meat developed in a collaborative effort with Biotage and Tyson Foods, Inc. Springdale Corporate Laboratory to optimize analyte recovery (>70%), minimize analyte suppression and maintain acceptable method precision. FAST LC-MS 2014

Aminoglycoside antibiotics Chicken meat Column English Food Gentamicin LC-MS/MS Neomycin Posters Spectinomycin Streptomycin Veterinary drugs

This poster describes a strong cation exchange SPE protocol for extraction of Vitamin B3 (Niacin) and related metabolites from serum. The ISOLUTE SCX-3 96 well plate format demonstrated as a viable option for serum measurements over a relevant concentration range in clinical diagnostics. It is anticipated that strategies presented in this poster would be of broad-based interest to clinical laboratories focused on combining parent/metabolite assays into a single analysis. MSACL 2014

96-well plate B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Posters Serum Vitamin B3 Vitamins

This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.

96-well plate Application Notes B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Serum Vitamin B3 Vitamins

This application note describes the extraction of nine phthalate metabolites from human urine using ISOLUTE® ENV+ solid phase extraction columns.

Application Notes Biomarker Clinical Column English LC-MS/MS Mono(2-ethyl-5-carboxypentyl) phthalate (MECPP) Mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono(2-ethylhexyl) phthalate (MEHP) Monobenzyl phthalate (MBzP) Monobutyl phthalate (MBP) Monoethyl phthalate (MEP) Monohexyl phthalate (MHxP) Monoisononyl phthalate (MiNP) Monomethyl phthalate (MMP) Phthalate Metabolites Phthalates Urine

The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS. This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS. MSACL 2016

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetyl codeine 96-well plate Acidic Alprazolam Amphetamine Barbiturates Basic Benzodiazepines Benzoylecgonine Buprenorphine Butalbital Clinical Clonazepam Codeine Dextromethorphan Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydrolysed urine Hydromorphone LC-MS/MS MDEA Morphine Naltrexone Neutral Nitrazepam Norbuprenorphine Norcodeine Norfentanyl Opiates Oxazepam Oxycodone Oxymorphone Pain Management Pentazocine Pentobarbital Phenobarbital Posters Propoxyphene Secobarbital THC THC metabolites THC-COOH Temazepam Δ9-tetrahydrocannabinol (THC)

Aflatoxins, a mold largely produced by Aspergillus flavus and Aspergillus parasiticus1 are commonly tested mycotoxins found naturally in a wide range of agriculture crops and food products. Due to their harmful effects on human health, animal health, and global trade, aflatoxins are regulated in most countries and have established global limits in a wide variety of matrices.

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Column English Food Food HPLC Mycotoxin

This poster describes a supported liquid extraction method for extraction of a class of novel psychoactive substances (NBOMes) with analysis using laser diode thermal desorption (LDTD) MS/MS. Samples are oral fluid collected using Salivettes. TAMS 2014

25B-NBOMe 25C-NBOMe 25D-NBOMe 25E-NBOMe 25H-NBOMe 25I-NBOMe 25N-NBOMe 25T2-NBOMe 96-well plate Drugs of Abuse English Forensic LDTD-MS/MS Legal highs NBOMes Oral fluid Phenethylamines Posters

This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated. ASMS 2014

96-well plate Biotin Clinical English LC-MS/MS Posters Serum Vitamin B7 Vitamins

This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance. AACC 2014

96-well plate Catecholamines Clinical English LC-MS/MS Metanephrine Normetanephrine Plasma Posters Urine

The compound 4 Methylimidazole (4-MeI) is made during the production of caramel color commonly found in foods and beverages, especially sodas. The Codex Alimentarius of the World Health Organization sets a limit of 250 mg/kg of 4-MeI. Also, the state of California has added 4-MeI to its Prop 65 list at an exposure level of 29 μg/day. This study looks at 4-MeI levels in sodas and malt sodas which are sold in and outside the state of California. The first section, included in this poster, describes the performance of automated solid phase extraction of 4-MeI using conventional HPLC/MS detection. ASMS 2018, San Diego, CA

4-Methylimidazole (4-MEI) Carbonated soft drink (soda) Column English Food Food contaminant LC-MS/MS Posters

The low-level detection required for cannabis use combined with the complexity of hair testing makes for a challenging application. This poster aims to demonstrate simplified sample preparation workflow for low level analysis of THC and metabolites from hair, using Biotage Lysera and ISOLUTE SLE+ columns for sample preparation. TIAFT 2019 SOFT 2019

Cannabidiol Cannabinoid Clinical Column English Forensic Hair LC-MS/MS Lysera Posters THC THC metabolites THC-COOH