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Extraction of Acidic Catecholamine Metabolites in Plasma Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis

31 January 2018

This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application delivers high analyte recoveries and excellent linearity in the range 10-200 mg/mL.

 

EVOLUTE® EXPRESS SPE products dramatically improve flow characteristics, and enhance sample preparation productivity.

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Analytes

  • Vanillylmandelic acid (VMA)
  • 5-hydroxyindoleacetic acid (5-HIAA)
  • Homovanillic acid (HVA)