A unique resin enabling the pharmaceutical industry to quickly develop and bring new complex peptide drugs candidates to market.
ChemMatrix® is a patented, 100% PEG (polyethylene glycol) based resin developed by PCAS BioMatrix that offers substantial advantages over traditional PEG based polystyrene resins for solid phase peptide synthesis. ChemMatrix overcomes the challenges of synthesizing longer and more complex therapeutic peptides. Peptides produced with ChemMatrix have higher purity and can be obtained with better yield.
ChemMatrix resin is made exclusively from primary ether bonds which are highly chemically stable.
No leaching occurs during synthesis and cleavage.
ChemMatrix resins have a loading of 0.4 – 0.6 mmol/g.
ChemMatrix allows the use of almost any kind of solvent, including water.
Available on request, ChemMatrix resin can be supplied with an extensive range of linkers for peptide acids, amides and fragments.
ChemMatrix resin has been field proven for easier and faster development of long, complex and hydrophobic peptides.
ChemMatrix resin was compared with polystyrene for the synthesis of many difficult peptides, and results clearly dem¬onstrate the advantages of the PEG resin. Here is an example where both resins were compared for the synthesis of the HIV-1 protease, a 99 amino acid peptide.
Cost-efficient synthesis of long peptides and complex sequences
Lower purification costs due to increased crude purity
High final recovery yield
Faster speed to market for new peptide drugs
ChemMatrix Resin Catalog
Biotage can supply grams to Kg quantities of the following popular ChemMatrix configurations:
ChemMatrix resins are designed to be used under identical conditions to polystyrene. The only difference is that due to the enhanced solvent and reagent accessibility of the ChemMatrix resin, which gives rise to superior reaction and chemistry performance, the resin may swell more in most solvents (including TFA).
All standard solvents used in SPPS such as DMF, NMP, DCM and MeOH are compatible with ChemMatrix resins (see Figure 1). The ChemMatrix resins are fully compatable with the Syro family of Biotage peptide instruments.
Comparison of ChemMatrix and polystyrene swelling profiles in a variety of SPPS solvents. The increased swelling in the case of ChemMatrix confers much greater solvent and reagent accessibility and thereby reduced the occurrence of by products or impurities such as incomplete couplings and deletion sequences. Please note that the following procedure can be further optimized with alternative coupling agents, depending on the coupling system and requirements, for examples: HBTU/HOBt or HCTU/6-Cl HOBt for PyBOP/HOBt or HATU/HOAt. N,N-diisopropylethylamine can also be replaced by weaker bases such as N-methylmorpholine if racemization is a specific risk to your peptide.
Please note that the following procedure can be further optimized with alternative coupling agents, depending on the coupling system and requirements, for examples: HBTU/HOBtor HCTU/6-Cl HOBt for PyBOP/HOBt or HATU/HOAt. N,N-diisopropylethylamine can also be replaced by weaker bases such as N-methylmorpholine if racemization is a specific risk to your peptide.
The cleavage step relies on the use of TFA. Depending on the peptide sequence, all kind of cleavage cocktails could be used. If Arg(Pbf) is present in the sequence, the cleavage should be left 3 hours instead of one.
ChemMatrix is a patented, 100% PEG (polyethylene glycol) based resin developed by PCAS BioMatrix that offers substantial advantages over traditional PEG based polystyrene resins for solid phase peptide synthesis.
Biotage has developed a range of tools to improve the peptide synthesis workflow, from synthesis of crude peptides through to the final purified product. These solutions make your workflow more efficient and improve the quality of peptides you synthesize. Our workflow solutions are used in pharma, biotech, CRO and academic laboratories throughout the world.
Relevant peptide associations. Please contact webmaster to add to, or update this page. Asia Pacific Protein Association Association of Biomolecular Resource Facilities American Peptide Societ...
09-17-2014Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
07-29-2014Biotage announces the addition of ChemMatrix® resins to its extensive offering of peptide synthesis and purification products. ChemMatrix offers substantial advantages over traditional PS and P...
12-06-2010Biotage announces the addition of ChemMatrix® resins to its extensive offering of peptide synthesis and purification products. ChemMatrix offers substantial advantages over traditional PS and P...
12-06-2010For peptide acids, we recommend using the HMPB-ChemMatrix as this resin will provide high crude purity and a recovery yield of 90-95%. The Wang-ChemMatrix will produce similar crude peptide purity, but the recovery yield is lower (60-70%).
Rink Amide ChemMatrix is one of the most popular resins for both manual solid phade peptide synthesis (SPPS) and automated SPPS, under microwave or non-microwave conditions.
Trityl-ChemMatrix is mainly used to obtain a protected peptide as is has very low TFA cleavage conditions (under 1%). This resin therefore has the same cleavage conditions as the Cl-Trityl-polystyrene. Please take note that the Cl-Trityl-ChemMatrix resin is not offered for stability reasons. It is more effective to start with the Trityl-ChemMatrix resin, chlorinate the resin and then add the amino acid required.
Wang ChemMatrix was the first ChemMatrix resin developed for peptide acids. It remains to this day one of the work-horses for solid phase peptide synthesis (SPPS).