ChemMatrix® is a patented, 100% PEG (polyethylene glycol) based resin developed by PCAS BioMatrix that offers substantial advantages over traditional PEG based polystyrene resins for solid phase peptide synthesis. ChemMatrix overcomes the challenges of synthesizing longer and more complex therapeutic peptides. Peptides produced with ChemMatrix have higher purity and can be obtained with better yield.

Exceptional Stability

ChemMatrix resin is made exclusively from primary ether bonds which are highly chemically stable.

Superior Properties

No leaching occurs during synthesis and cleavage.

High Loadings

ChemMatrix resins have a loading of 0.4 – 0.6 mmol/g.

Solvent Compatibility

ChemMatrix allows the use of almost any kind of solvent, including water.

Versatile Choices

Available on request, ChemMatrix resin can be supplied with an extensive range of linkers for peptide acids, amides and fragments.

Demonstrated Superiority

ChemMatrix resin has been field proven for easier and faster development of long, complex and hydrophobic peptides.

Higher Purity Peptides

ChemMatrix resin was compared with polystyrene for the synthesis of many difficult peptides, and results clearly dem¬onstrate the advantages of the PEG resin. Here is an example where both resins were compared for the synthesis of the HIV-1 protease, a 99 amino acid peptide.

Superior Performance Makes ChemMatrix an Exceptional Value

Cost-efficient synthesis of long peptides and complex sequences
Lower purification costs due to increased crude purity
High final recovery yield
Faster speed to market for new peptide drugs
ChemMatrix Resin Catalog
Biotage can supply grams to Kg quantities of the following popular ChemMatrix configurations:

• Rink Amide-ChemMatrix – Amide
• HMPB-ChemMatrix – Acid
• Trityl OH-ChemMatrix – Acid
• Wang-ChemMatrix – Acid
• PAL-ChemMatrix – Amide
• For special requirements, contact us to discuss resins customized to your specific needs.

Biotage Provides Complete Support, Worldwide

• Extensive polymer knowledge – Our technical team has over 10 years of experience developing 100% PEG resins
• Full technical support – We can work closely with you in all phases of peptide processing and development
• Uncompromising product quality – Resins produced in ISO9001 and cGMP facilities
• Resins available in small quantities to industrial scale
• Strong QC and regulatory affairs support

General Procedures

Solid Phase Peptide Synthesis (SPPS) using ChemMatrix® Resins

ChemMatrix resins are designed to be used under identical conditions to polystyrene. The only difference is that due to the enhanced solvent and reagent accessibility of the ChemMatrix resin, which gives rise to superior reaction and chemistry performance, the resin may swell more in most solvents (including TFA).

All standard solvents used in SPPS such as DMF, NMP, DCM and MeOH are compatible with ChemMatrix resins (see Figure 1). The ChemMatrix resins are fully compatable with the Syro family of Biotage peptide instruments.

Figure 1.

Comparison of ChemMatrix and polystyrene swelling profiles in a variety of SPPS solvents. The increased swelling in the case of ChemMatrix confers much greater solvent and reagent accessibility and thereby reduced the occurrence of by products or impurities such as incomplete couplings and deletion sequences. Please note that the following procedure can be further optimized with alternative coupling agents, depending on the coupling system and requirements, for examples: HBTU/HOBt or HCTU/6-Cl HOBt for PyBOP/HOBt or HATU/HOAt. N,N-diisopropylethylamine can also be replaced by weaker bases such as N-methylmorpholine if racemization is a specific risk to your peptide.

Recommended Protocol – General Advice

Please note that the following procedure can be further optimized with alternative coupling agents, depending on the coupling system and requirements, for examples: HBTU/HOBtor HCTU/6-Cl HOBt for PyBOP/HOBt or HATU/HOAt. N,N-diisopropylethylamine can also be replaced by weaker bases such as N-methylmorpholine if racemization is a specific risk to your peptide.

The cleavage step relies on the use of TFA. Depending on the peptide sequence, all kind of cleavage cocktails could be used. If Arg(Pbf) is present in the sequence, the cleavage should be left 3 hours instead of one.

Coupling step (Representative Example)

• Swell the resin with DMF (10 mL/g of resin);
• Dissolve the Fmoc-amino acid (3 eq.) and HCTU (3 eq.) in a minimum of DMF followed by the addition of NMM (N-Methylmorpholine) (4-5 eq.);
• Add this solution to the swelled resin;
• Stir 30 minutes at room temperature;
• Filter and wash with DMF (3-4 times);
• Add a solution of 20% piperidine in DMF (10-12 mL/g) to the resin and stir 30 minutes;
• Filter and wash with DMF (3-4 times);
• Begin the coupling of a new amino acid.

Cleavage step (Representative Example)

• Following coupling reaction, wash the resin with methanol and then with Et2O;
• Dry the resin for several hours under vacuum at room temperature;
• Add 20 mL/g of a solution of TFA / H2O / Et3SiH (1-10/96-87/2.5) 
• and stir one hour (3 hours if Arg(Pbf) are present);
• Guideline: TFA + (H2O or DCM) = 97.5%
• Filter and rinse the resin twice with TFA;
• Evaporate all the solvents to a minimum;
• Under stirring, add cold Et2O (10-15 mL/mL of residual volume of solvent with the peptide);
• Centrifugate the suspension and remove the supernatant ether;
• Repeat step 7 TWICE more;
• Dry the peptide under vacuum overnight (freeze drying could be also performed with a suitable solvent).