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The Industry Standard Original Process Scale Purification
When considering larger, production scale equipment, robustness and safety are key factors for any device. The Flash 75/150/400 systems need minimal maintenance and their design has proven to be extremely reliable over many years. These systems allowing operators to save hours or even days of separation time.
Biotage® Flash 75/150
Purify up to 400 grams of compound at 1L/min up to 75% faster than with traditional glass columns. Biotage® Flash 75/150 is a simple and reliable system that contains everything needed for separation scale up.
Biotage® Flash 400
The Biotage® Flash 400 is a complete skid-mounted system designed for kilogram separations. Built to last and engineered to perform, Flash 400 systems are the first choice of companies around the world for critical adsorption purification.
APPLICATION NOTES
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
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Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases
The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. Luckily, there is technology that solves this problem.
DMSO and DMF as Injection Solvents
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Impact of Injection Solvent Choice on Peak Shape and Resolution in Reversed-phase Flash Chromatography
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration. ACS 2016
Options Available for Green Flash Chromatography
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed. ACS 2016.
Greener reversed-phase Flash Chromatography Using Acetone Instead of Acetonitrile
As reversed-phase flash chromatography gains traction in medicinal chemistry labs the need to monitor its cost and safety are becoming more important. Commonly used reversed-phase solvents typically include water with an organic solvent such as methanol or acetonitrile – each have advantages and disadvantages.
Acetonitrile as a Replacement for Methanol in Normal-phase Flash Chromatography
When it comes to polar organic compound purification, many chemists turn to normal-phase flash chromatography often utilizing dichloromethane and methanol as the eluting solvents. While this can work, it often can be challenging to optimize due to methanol’s high polarity and protic chemistry.
Strategies for Improving Flash Chromatography Efficiency
For most organic and natural product chemists flash chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals. In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Mass-Directed Purification of Natural Products
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
White Paper A Four Part Approach to Reducing the Environmental Impact of Purification Chromatography
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the environmental impact of their work.
White Paper Reducing the Bottleneck in Target Synthesis
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced accordingly.
Improving Peptide Purification via Flash Column Chromatography by Modulating Mobile Phase pH
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Alternative Strategies for Purification of Fully Protected Peptides Using Flash Chromatography
Improvements in solid phase peptide synthesis strategies and development of resin linkages susceptible to low acid cleavage conditions has enabled synthesis of long peptides while keeping the protecting groups intact. This strategy is now used for the preparation of chemically synthesized proteins, wherein shorter peptide fragments are ligated together. They are also found in the synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Biotage® Flash 400 System
The Biotage® Flash 400 system is a complete skid-mounted system designed for kilogram scale separations. Built to last and engineered to perform, with materials that comply with FDA regulations and cGMP standards, Flash 400 is the first choice of pharmaceutical and contract manufacturing companies around the world for critical purification applications.
Biotage® Flash 75/150
Purify up to 400 grams of compound at 1L/min up to 75% faster than with traditional glass columns. Biotage® Flash 75/150 is a simple and reliable system that contains everything you need for separation scale up.
