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- Biotage® Sfär - Overview
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Biotage® Sfär, Sfär for Spherical, Biotage for Quality
Biotage was the first company to pre-pack flash chromatography columns, and continues to be the innovation leader with the new Biotage® Sfär columns.
Sfär is the Swedish word for "sphere", and the name of our columns reflects the fact that we have made spherical silica a standard due to its higher surface area and higher loading capacity. Reliable and flexible, Biotage® Sfär columns deliver larger loading capacities, tighter elution bands and purer fractions than traditional flash columns.
PRODUCT DESCRIPTIONS
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase chromatography that provides an efficient mechanism for the separation of polar compounds.
OTHER
Why Reusing Flash Cartridges is Bad Science
To save money on consumables, many chemists choose to reuse silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems. Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Organic Chemistry - Products from Start to Finish
Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.
Biotage® Sfär Columns From the Pioneers of Flash Purification
Biotage Sfär columns are quality tested to ensure they meet stringent performance criteria including efficiency and peak symmetry. Each CE-marked column is built using inert, foodgrade plastics for lower extractables, cleaner fractions, and packed to provide excellent resolution.
Erfolg mit Flash-Chromatographie
Die Flash-Chromatographie ist die bevorzugte Reinigungsmethode für organische Stoffe, Arzneimittel und Naturstoffe. In jüngster Zeit hat sie auch die Peptidchemie erobert, verfügt sie doch über die Fähigkeit, eine Vielzahl unterschiedlicher Verbindungen effizienter zu trennen, als dies mit anderen Vorreinigungsverfahren wie z. B. dem Ausfällen (Protein-Crash) oder der Flüssig-Flüssig-Extraktion möglich ist. Zur Herstellung reiner Verbindungen können Chemiker je nach dem gewünschten Reinheitsgrad auf eine Vielzahl unterschiedlicher Variablen zurückgreifen. In diesem Whitepaper möchten wir die Faktoren erläutern, die für eine erfolgreiche Aufreinigung mithilfe der Flash-Chromatographie kontrolliert werden müssen.
Biotage® Sfär Samplet® vs. Biotage® SNAP Samplet® - What Goes Where?
It is important to understand there are design differences between SNAP and Sfär Samplets, and that SNAP Samplets cannot be used on Sfär columns and vice versa. See samplet-column compatibility table on the next page.
Green Flash Chromatography
Flash chromatography, a staple component of medicinal chemistry workflow, consumes a lot of organic solvent (upwards of half a million liters annually in just North America). Most, of this organic waste is incinerated off-site, liberating CO2 into the atmosphere. Because of this environmental impact, many companies are instituting requirements to reduce organic solvent waste but leaving the implementation to their chemistry departments. In this poster, we describe several proven ways to reduce solvent use without sacrificing purification efficiency.
Phase out Isolera Four
APPLICATION NOTES
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
PRODUCT VIDEOS
How to Manually add Biotage® Sfär Columns to Isolera™
News
Webinar: A Roadmap to Successful Flash Chromatography
Want to improve your flash chromatography skills? Learn all the tricks of the trade in this one-hour webinar with flash guru Bob Bickler. For a dedicated synthetic chemist, the goal ...
08-09-2018Media Porosity, Why Should I Care?
"Normal-phase silica has, on average, 50 to 60 Å pores (and a surface area of 500 m²/g). However, for small-molecule reversed-phase chromatography to be useful, the average pore siz...
05-31-2017Scaling Up Flash Purification – Easy as 1, 2, 3, 4
This blog post on the Flash Purification Blog discusses the process of simplified flash purification scale-up. For many chemists performing bench-scale organic synthesis, flash column chro...
11-13-2015New Blog Post: How do I Choose the Right Column Size for Purification by Flash Chromatography?
Techincal specialist Bob Bickler goes through a good method to optimize column size for your purification in his latest blog post on The Flash Purification Blog. "In all my years of work...
09-01-2015Tutorial Video – How to Load Flash Chromatography Cartridges
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
06-30-2015Why Reusing Flash Cartridges is Bad Science
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity. To save money on consumables, many chemists choo...
12-04-2014Isolera™ Used in Recent Natural Products Study
The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
09-12-2014Difficult Fractionation of Lipids Made Easy with Isolera™ Dalton Flash Purification
Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
08-06-2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
07-29-2014The Lines Between Purification and Analysis are Blurring…
By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
03-14-2014Purifying the First Hundred Grams
Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
10-18-2011Biotage® Sfär - Overview
Sfär is the Swedish word for "sphere", and the name of our columns reflects the fact that we have made spherical silica a standard due to its higher surface area and higher loading capacity. Reliable and flexible, Biotage® Sfär columns deliver larger loading capacities, tighter elution bands and purer fractions than traditional flash columns.
Biotage® Sfär - Samplet®
Creating dried, adsorbed samples will improve the separation performance on any cartridge. Our Samplet® are designed for quick and convenient loading of concentrated samples. After the sample has been applied to the Samplet®, the solvent is allowed to evaporate. The Samplet is then inserted into the Biotage® Sfär Duo columns and you are ready to go.
TLC Plates
TLC is a commonly used method development tool for flash purification. Product mixtures separated using TLC generally can be purified by flash chromatography using the identical solvent system.
