
The SNAP KP-NH columns in the following sizes have been phased out on October 1st, 2019: 11g, 28g, 55g, 110g, 375g.
Please check out here the corresponding column in the Biotage® Sfär family: Biotage® Sfär Amino Duo
The following formats remain available: 900g, 1800g.
The large-scale SNAP cartridges are targeted to the development-scale chemists synthesizing and/or purifying from 10g to 100+ grams of product across a wide range of chemistries or conditions, and providing seamless scale-up simply and efficiently without method modification. The SNAP XL cartridges will cater for almost all eventualities in large scale purification of small organic molecules.
SNAP 900 g and 1800 g cartridges are inert, rugged, high-throughput flash cartridges designed to purify large synthetic sample mixtures from tens to hundreds of grams across a range of different chemistries.
SNAP XL enables chemists to use columns designed for large scale applications instead of using two or three smaller cartridges, with a significant time and cost benefit vs running the same separation with two or more smaller columns.
SNAP XL are rugged and laser welded for safe operation at pressures up to 100 psi(7 bar), enabling high flow rates and use of higher viscosity solvents, and connectivity is done via standard luer lock connections. Operating at flow rates up to 500 mL/min, these cartridges allow to quickly purify large sample masses, saving hours or even days of purification runtime.
A refillable external dry load vessel with 500g capacity is also available to be attached in series with the SNAP 900g or SNAP 1800g cartridge.
Sizes | 900g, 1800g |
Media | Biotage® KP-NH - 50μm irregular silica |
Compatibility | Universal Luer connections |
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
Introduction
Research in CNS drugs is primarily centered on nitrogen heterocycle chemistry. Basic amines are difficult to purify using traditional silica chromatography because of strong interactions between acidic silica and the molecules’ basic amine groups. These interactions cause band spreading and poor compound recovery. Solutions employed to counteract this phenomena include adding a competing amine (e.g. triethyl amine or ammonium hydroxide) to the flash chromatography solvent system or using reversed-phase HPLC with a buffered solvent system.
Tags: Application Notes
Biotage Sfär columns are quality tested to ensure they meet
stringent performance criteria including efficiency and peak
symmetry. Each CE-marked column is built using inert, foodgrade
plastics for lower extractables, cleaner fractions, and
packed to provide excellent resolution.
Tags: Brochures
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
Tags: Brochures
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
It is important to understand there are design differences between
SNAP and Sfär Samplets, and that SNAP Samplets cannot be used on
Sfär columns and vice versa. See samplet-column compatibility table on the next page.
Tags: Manuals & User Guides,
Product Notes
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
Tags: Manuals & User Guides
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
Biotage KP-NH TLC plates come in a 10 x 10 cm format. The glass has been pre-scored to provide four 2.5 x 10 cm plates when appropriately snapped.
Tags: Manuals & User Guides
Safety Data Sheet
Tags: English,
Material Safety Data Sheets
Flash chromatography, a staple component of medicinal chemistry workflow, consumes a lot of organic solvent (upwards of half a million liters annually in just North America). Most, of this organic waste is incinerated off-site, liberating CO2 into the atmosphere. Because of this environmental impact, many companies are instituting requirements to reduce organic solvent waste but leaving the implementation to their chemistry departments.
In this poster, we describe several proven ways to reduce solvent use without sacrificing purification efficiency.
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The results from this research show that the chromatographic purification of organic amine compounds can be improved simply by using an amine-functionalized silica and typical normal-phase solvents such as hexane and ethyl acetate.
Tags: Posters
It is important to understand there are design differences between
SNAP and Sfär Samplets, and that SNAP Samplets cannot be used on
Sfär columns and vice versa. See samplet-column compatibility table on the next page.
Tags: Manuals & User Guides,
Product Notes
To save money on consumables, many chemists choose to reuse
silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems.
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Tags: Product Notes
SNAP KP-NH cartridges contain amine functionalized silica.
This optimized media shields synthetic organic amines from
acidic silanols providing improved selectivity, peak shape,
purity and yield (Figure 1). Unlike traditional silica and 1°
amine (propyl amine) bonded silica, KP-NH doesn’t require the
use of chlorinated solvents or amine additives.
Tags: Product Notes
Die Flash-Chromatographie ist die bevorzugte
Reinigungsmethode für organische Stoffe, Arzneimittel und
Naturstoffe. In jüngster Zeit hat sie auch die Peptidchemie
erobert, verfügt sie doch über die Fähigkeit, eine Vielzahl
unterschiedlicher Verbindungen effizienter zu trennen, als dies
mit anderen Vorreinigungsverfahren wie z. B. dem Ausfällen
(Protein-Crash) oder der Flüssig-Flüssig-Extraktion möglich ist.
Zur Herstellung reiner Verbindungen können Chemiker je nach
dem gewünschten Reinheitsgrad auf eine Vielzahl unterschiedlicher
Variablen zurückgreifen. In diesem Whitepaper möchten
wir die Faktoren erläutern, die für eine erfolgreiche Aufreinigung
mithilfe der Flash-Chromatographie kontrolliert werden müssen.
Tags: White Papers
Dear Valued Customers, We value your continued support and therefore want to notify you of a change in our product line - the discontinuation of the Biotage® SNAP and Biotage® SNAP Ultra p...
09 August 2018Want to improve your flash chromatography skills? Learn all the tricks of the trade in this one-hour webinar with flash guru Bob Bickler. For a dedicated synthetic chemist, the goal ...
31 May 2017"Normal-phase silica has, on average, 50 to 60 Å pores (and a surface area of 500 m²/g). However, for small-molecule reversed-phase chromatography to be useful, the average pore siz...
13 November 2015This blog post on the Flash Purification Blog discusses the process of simplified flash purification scale-up. For many chemists performing bench-scale organic synthesis, flash column chro...
01 September 2015Techincal specialist Bob Bickler goes through a good method to optimize column size for your purification in his latest blog post on The Flash Purification Blog. "In all my years of work...
26 August 2015A poster from Biotage presented at ACS 2015 in Boston shows how a unique amine-functionalized silica simplifies and improves purification of several basic tricyclic antidepressants with pKa values ...
30 June 2015Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
04 June 2015If you are making amine functionalized organic molecules, and you find it challenging to purify them with normal-phase silica, you are not alone. If you synthesize organic amine...
04 December 2014Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity. To save money on consumables, many chemists choo...
12 September 2014The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
06 August 2014Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
29 July 2014Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
14 March 2014By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
18 October 2011Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
Biotage® KP-NH chemistry shields synthetic organic amines from acidic silanols providing improved selectivity, peak shape, purity and yield. Unlike traditional silica and 1° amine (propyl amine) bonded silica, Biotage KP-NH doesn’t require the use of chlorinated solvents or amine additives.
Biotage® SNAP KP-NH flash cartridges aseparate 2°, 3°, and heterocyclic amines using non-chlorinated solvents.
The Luer-lock connections operate safely at 100 psi without compression modules and can be used on any flash system.
Part No. | Description | Product type | Pack Size | Price | ||
DLV-505 | Empty dry load cartridges and frits, 4/cs | Consumables | 4 | Log in for price | ||
DLV-TF | Top frit for DLV-500 | Consumables | 10 | Log in for price | ||
FSN0-0909-0900 | Biotage® SNAP KP-NH 900 g, 1/cs | Consumables | 1 | Log in for price | ||
FSN0-0909-1800 | Biotage® SNAP KP-NH 1800 g, 1/cs | Consumables | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
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DLV-500 | Dry Load Vessel for Isolera LS, 1/cs | Accessories | 1 | Log in for price | |
DLV-510 | O-ring DLV500 | Accessories | 5 | Log in for price |