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- Automated Sample Preparation Instruments
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- ISOLUTE® FILTER+ High Performance Filtration Plates
- ISOLUTE® PPT+ Protein Precipitation Plates
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
- ISOLUTE® QuEChERS
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- ISOLUTE® SPE Columns and Plates
-
- ISOLUTE® 101
- ISOLUTE® AL-A, AL-N & AL-B
- ISOLUTE® Accessories
- ISOLUTE® C18
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- EVOLUTE® SPE Columns and Plates
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- Biotage® Selekt Systems
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- Flash Columns
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- Biotage® Sfär - Overview
- Biotage® Sfär Silica HC D
- Biotage® Sfär Silica D
- Biotage® Sfär Silica HC
- Biotage® Sfär Silica
- Biotage® Sfär KP-Amino D
- Biotage® Sfär C18 Duo 100 Å 30 μm
- Biotage® Sfär - Samplet®
- Biotage® SNAP XL KP-Sil
- Biotage® SNAP XL KP-NH
- Biotage® SNAP XL Ultra C18
- Biotage® SNAP XL KP-C18-HS
- Flash 75/150/400 Cartridges
- TLC Plates
- Flash Accessories
- Flash 75/150/400 Systems
- Service Agreements - Europe
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- Discontinued Products
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- Biotage® SNAP Bio
- XcelVap®
- Biotage® SNAP Ultra
- Biotage® SNAP - Samplet®
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- EcoLine Environmental Filters
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- KILOPREP Systems and Consumables
- RapidTrace®+
- SAM
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- Speed-Vap® III
- TurboVap® 96
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- TurboVap® Classic LV
Biotage® SNAP XL Ultra C18
Increasing Reversed Phase Resolution and Loading
The SNAP Ultra C18 columns have been phased out on October 1st, 2019 in the following sizes: 12g, 30g, 60g, 120g, 400g.
Please check out here the corresponding column in the Biotage® Sfär family: Biotage® Sfär C18 Duo 100 Å 30 μm
The following formats remain available: 950g, 1850g.
The large-scale SNAP cartridges are targeted to the development-scale chemists synthesizing and/or purifying from 10g to 100+ grams of product across a wide range of chemistries or conditions, and providing seamless scale-up simply and efficiently without method modification. The SNAP XL cartridges will cater for almost all eventualities in large scale purification of small organic molecules.
SNAP 950 g and 1850 g cartridges are inert, rugged, high-throughput flash cartridges designed to purify large synthetic sample mixtures from tens to hundreds of grams across a range of different chemistries.
SNAP XL enables chemists to use columns designed for large scale applications instead of using two or three smaller cartridges, with a significant time and cost benefit vs running the same separation with two or more smaller columns.
SNAP XL are rugged and laser welded for safe operation at pressures up to 100 psi (7 bar), enabling high flow rates and use of higher viscosity solvents, and connectivity is done via standard luer lock connections. Operating at flow rates up to 500 mL/min, these cartridges allow to quickly purify large sample masses, saving hours or even days of purification runtime.
A refillable external dry load vessel with 500g capacity is also available to be attached in series with the SNAP 950g or SNAP 1850g cartridge.
| Sizes | 950 g, 1850 g |
| Media | Biotage® HP-Sphere C18 - 25 micron spherical silica |
» APPLICATION NOTES
Fully Automated Optimized Synthesis, On-resin Oxidation, and Purification of Isotopically Labeled Oxytocin
This application note describes optimization for a fully automated synthesis and on-resin cyclization of oxytocin enabled by Branches™, a unique software feature of the Biotage® Initiator+ Alstra™ peptide synthesizer.
Tags: Application Notes
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
Reversed Phase Purification of Natural Products
Reversed-phase chromatography facilitates the isolation of milligram to multi-gram quantities of polar compounds from naturally occurring materials or from synthetic reaction
products. Biotage reversed phase KP-C18-HS SNAP cartridges have been shown in a number of applications to be effective for the purification of natural products. The methodology is robust and may
be applied generically to new extraction purification runs.
Keywords: natural, products, reversed, phase, normal, extraction, spinach.
Tags: Application Notes,
English
Real-time Fraction Purity Analysis in Reversed-phase Flash Chromatography
Pure fractions are in high demand – impurities mean more work after purification. With new technology, fraction purity can be digitally analyzed directly during chromatography to reveal any problems on the fly. In this application we will show how the Isolera Spektra is used to determine fraction purity, eliminating the need for other post-purification analysis.
Tags: Application Notes,
English
Improving Reversed-Phase FLASH Purification Throughput
Normal-phase flash purification is commonly used by organic chemists in pharmaceutical drug discovery and
process development labs. However, for many synthesized products (e.g. peptides, nucleotides and basic
drug candidates) purification on standard flash silica is not an option due to irreversible adsorption, chemical
interaction and/or solubility issues. Reversed-phase flash purification is an excellent solution for these applications. Yet, this technique has been used sparingly because of perceived lower loading capacity, higher
operating pressures and a scarcity of publications addressing reversed-phase flash chromatography.
Tags: Application Notes
» BROCHURES
Biotage® Sfär Columns From the Pioneers of Flash Purification
Biotage Sfär columns are quality tested to ensure they meet
stringent performance criteria including efficiency and peak
symmetry. Each CE-marked column is built using inert, foodgrade
plastics for lower extractables, cleaner fractions, and
packed to provide excellent resolution.
Tags: Brochures
Organic Chemistry - Products from Start to Finish
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
Tags: Brochures
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
» MANUALS & USER GUIDES
Biotage® Sfär Samplet® vs. Biotage® SNAP Samplet® - What Goes Where?
It is important to understand there are design differences between
SNAP and Sfär Samplets, and that SNAP Samplets cannot be used on
Sfär columns and vice versa. See samplet-column compatibility table on the next page.
Tags: Manuals & User Guides,
Product Notes
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
Tags: Manuals & User Guides
Reversed-Phase Flash Purification
Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this
area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase
chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes,
Brochures,
English,
Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet HP Sphere C18, SNAP Ultra, C18
Laboratory chemical Not for therapeutic or diagnostic use
Tags: Material Safety Data Sheets
» POSTERS
Green Flash Chromatography
Flash chromatography, a staple component of medicinal chemistry workflow, consumes a lot of organic solvent (upwards of half a million liters annually in just North America). Most, of this organic waste is incinerated off-site, liberating CO2 into the atmosphere. Because of this environmental impact, many companies are instituting requirements to reduce organic solvent waste but leaving the implementation to their chemistry departments.
In this poster, we describe several proven ways to reduce solvent use without sacrificing purification efficiency.
Tags: Posters
Investigating the use of Mixed Stationary Phases for Peptide Purification via Reversed Phase Flash Chromatography
Peptide purification using standard reversed phase HPLC methods are hampered by low loading capacity, resulting in purifications that demand significant time investment. Recently, the use of reversed phase flash chromatography has increased in popularity for peptide purification due to the significant reduction of purification time,
enabled by the increased loading levels of the larger stationary phase particles. Resolution, though, is somewhat diminished with the larger particle size, demanding creative techniques to retain a highly pure peptide product.
Tags: Posters
DMSO and DMF as Injection Solvents
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Tags: Posters
New High-performance Biotage® C18 Flash Cartridge Significantly Improves Resolution and Fraction Purity
In this poster we will show how a new high-performance reversed-phase flash cartridge increases separation power and increases loading capacity 2x over conventional C18 cartridges.
Tags: Posters
» PRODUCT NOTES
Biotage® Sfär Samplet® vs. Biotage® SNAP Samplet® - What Goes Where?
It is important to understand there are design differences between
SNAP and Sfär Samplets, and that SNAP Samplets cannot be used on
Sfär columns and vice versa. See samplet-column compatibility table on the next page.
Tags: Manuals & User Guides,
Product Notes
White Paper - Den Engpass in der Target-Synthese überwinden
Modelle haben gezeigt, dass sich die zur Herstellung
eines Zielmoleküls benötigte Zeit mit dem automatisierten
Workflow von Biotage um bis zu achtzig
Prozent verkürzen lässt, sodass auch die Projektlaufzeiten
entsprechend verkürzt werden können.
Tags: German,
Product Notes,
White Papers
Biotage® SNAP ULTRA C18 Cartridges
Increased Resolution and Loading Capacity.
The latest, highest performing reversed phase C18
spherical silica flash cartridges available.
Tags: Product Notes
Why Reusing Flash Cartridges is Bad Science
To save money on consumables, many chemists choose to reuse
silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems.
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Tags: Product Notes
Biotage® SNAP Reversed Phase Cartridges
Reversed-phase flash chromatography is a very popular
purification technique using a non-polar stationary phase.
Main application areas include separation of polar, ionizable
and highly lipophilic compounds which cannot easily be
separated by normal-phase techniques.
Tags: Product Notes
» WHITE PAPERS
Erfolg mit Flash-Chromatographie
Die Flash-Chromatographie ist die bevorzugte
Reinigungsmethode für organische Stoffe, Arzneimittel und
Naturstoffe. In jüngster Zeit hat sie auch die Peptidchemie
erobert, verfügt sie doch über die Fähigkeit, eine Vielzahl
unterschiedlicher Verbindungen effizienter zu trennen, als dies
mit anderen Vorreinigungsverfahren wie z. B. dem Ausfällen
(Protein-Crash) oder der Flüssig-Flüssig-Extraktion möglich ist.
Zur Herstellung reiner Verbindungen können Chemiker je nach
dem gewünschten Reinheitsgrad auf eine Vielzahl unterschiedlicher
Variablen zurückgreifen. In diesem Whitepaper möchten
wir die Faktoren erläutern, die für eine erfolgreiche Aufreinigung
mithilfe der Flash-Chromatographie kontrolliert werden müssen.
Tags: White Papers
White Paper - Den Engpass in der Target-Synthese überwinden
Modelle haben gezeigt, dass sich die zur Herstellung
eines Zielmoleküls benötigte Zeit mit dem automatisierten
Workflow von Biotage um bis zu achtzig
Prozent verkürzen lässt, sodass auch die Projektlaufzeiten
entsprechend verkürzt werden können.
Tags: German,
Product Notes,
White Papers
Search all documents »
PRODUCT VIDEOS
How to Manually add Biotage® Sfär Columns to Isolera™
News
28 June 2019Notice of Discontinuation of Biotage® SNAP Flash Columns
Dear Valued Customers, We value your continued support and therefore want to notify you of a change in our product line - the discontinuation of the Biotage® SNAP and Biotage® SNAP Ultra p...
09 August 2018Webinar: A Roadmap to Successful Flash Chromatography
Want to improve your flash chromatography skills? Learn all the tricks of the trade in this one-hour webinar with flash guru Bob Bickler. For a dedicated synthetic chemist, the goal ...
31 May 2017Media Porosity, Why Should I Care?
"Normal-phase silica has, on average, 50 to 60 Å pores (and a surface area of 500 m²/g). However, for small-molecule reversed-phase chromatography to be useful, the average pore siz...
06 February 2017"That is why we bought the second Alstra"
Dr. Andrew Jamieson leads a team of peptide chemists at the University of Glasgow, where they synthesize complex molecules for chemical biology applications. An important tool that contributes to t...
19 January 2017Flash Purification of Peptides - Part 2
Biotage® SNAP Bio flash cartridges were developed with a small particle size (20 μm) and large pore size (~300 Å) to provide increased resolution and effective separation of complex pe...
10 January 2017Flash Purification of Peptides - Is it Possible?
Recent advances in flash purification using 20–25 micron spherical particles makes flash chromatography an efficient technique for synthetic peptide purification. This application note from B...
13 December 2016Webinar on Demand: Peptide Purification using Flash Chromatography
This webinar discusses some recent work on the use of flash chromatography for the purification of synthetic peptides as a complementary alternative to standard RP-HPLC methods. Included herein are...
01 November 2016Effective Cannabinoid Purification by Flash Chromatography
Results of an orthogonal flash purification approach using normal-phase followed by reversed-phase flash chromatography of a cannabis extract is reported in a poster from Biotage, presented at ACS ...
23 September 2016How do I Determine Loading Capacity in Reverse Phase Flash Column Chromatography?
In the world of HPLC, loading capacity isn’t normally a concern as it is primarily an analytical technique. In the synthetic organic chemistry world, most purification is performed with silic...
18 March 2016Isolera Flash Chromatography System Explained in German
The industry standard platform for flash purification, Isolera™, is given a basic presentation by our German account manager in this short video. Isolera™ ist ein Flash C...
02 March 2016So, What Exactly is a Column Volume in Flash Chromatography?
The term column volume, typically abbreviated CV, is a value used to help determine separation quality and loading capacity. But how can this concept and its definition be better understood? Bob Bi...
15 January 2016How to Develop Reversed-phase Flash Column Chromatography Methods
Method transfer from reversed phase TLC to reversed phase flash column chromatography can be very challenging. Simply put, the process is to create a gradient on an HPLC system and t...
13 November 2015Scaling Up Flash Purification – Easy as 1, 2, 3, 4
This blog post on the Flash Purification Blog discusses the process of simplified flash purification scale-up. For many chemists performing bench-scale organic synthesis, flash column chro...
11 September 2015How do I Choose Between Normal and Reversed Phase Flash Columns
A new post on the Flash Purification Blog addresses the question "Normal or reversed phase in flash chromatography". There is nothing more important to purification success with chromatogr...
01 September 2015New Blog Post: How do I Choose the Right Column Size for Purification by Flash Chromatography?
Techincal specialist Bob Bickler goes through a good method to optimize column size for your purification in his latest blog post on The Flash Purification Blog. "In all my years of work...
30 June 2015Tutorial Video – How to Load Flash Chromatography Cartridges
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
29 May 2015The Benefits of SNAP Ultra C18 Flash Columns
Find out about the key points of Biotage® SNAP Ultra C18 reversed-phase flash purification cartridges in this three minute video. Small molecules synthesized in drug discovery are incr...
14 May 2015Isolera™ Used to Discover New Metabolites from Fungi
A research group looking for new compounds in fungi used an Isolera™ flash purification system to purify the crude extracts. Fungi are among the most astonishing life forms on ...
09 April 2015Pure Fractions with New C18 Reversed Phase Flash Cartridge
The small particle media in Biotage® SNAP Ultra C18 flash cartridges double the sample load and increase fraction purity in preparative flash chromatography. This is shown in a new poster from ...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
11 March 2015A Customer's Perspective on Biotage Flash Products
Chugai Pharmaceutical in Japan have worked through nearly every piece of flash equipment from Biotage. Here they explain why - and what they expect from the future. Three research ch...
04 February 201522nd SCT Young Researchers, Paris
Biotage will attend the 22nd SCT Young Researchers in Paris, France 4-6 February 2015 Read More http://www2.sct-asso.fr/form.php?langue=english&cle_menus=1187970162
04 December 2014Why Reusing Flash Cartridges is Bad Science
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and fraction purity. To save money on consumables, many chemists choo...
24 November 2014Reversed Phase Purification Webinar On Demand
A recording of the webinar Reversed Phase Purification: Bridging the Gap From Flash to Prep HPLC is released on our Webinar channel for online viewing and download. Watch this...
20 November 2014The Definitive Flash Cartridge Guide
Which cartridge should I use? How do different silica gels influence my purification? What is the correlation between the Rf value and column volume? What's the difference between isocratic and ste...
06 November 2014Strong Test Results of New Biotage® SNAP Ultra C18 Flash Cartridges
Peakdale Molecular are impressed after beta testing of the new Biotage® SNAP Ultra C18 Cartridges for reversed phase flash chromatography. Using a 30 g Biotage® SNAP Ultra C18 colu...
30 October 2014New Biotage® SNAP ULTRA C18 Cartridges – High Performance Reversed Phase Flash Purification
Biotage® SNAP ULTRA C18 reversed phase cartridges are optimized for use with Biotage ACI™ and Isolera™ flash systems and are available in 12 g, 30 g, 60 g, 120 g, 400 g, 950 g and 1...
12 September 2014Isolera™ Used in Recent Natural Products Study
The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
06 August 2014Difficult Fractionation of Lipids Made Easy with Isolera™ Dalton Flash Purification
Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
14 March 2014The Lines Between Purification and Analysis are Blurring…
By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
18 October 2011Purifying the First Hundred Grams
Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
| DLV-505 | Empty dry load cartridges and frits, 4/cs | Consumables | 4 | Log in for price | ||
| DLV-TF | Top frit for DLV-500 | Consumables | 10 | Log in for price | ||
| FSUL-0401-0950 | Biotage® SNAP Ultra C18 950 g, 1/cs | Consumables | 1 | Log in for price | ||
| FSUL-0401-1850 | Biotage® SNAP Ultra C18 1850 g, 1/cs | Consumables | 1 | Log in for price | ||
ACCESSORIES
| Part No. | Description | Product type | Pack Size | Price | ||
|
DLV-500 | Dry Load Vessel for Isolera LS, 1/cs | Accessories | 1 | Log in for price | |
| DLV-510 | O-ring DLV500 | Accessories | 5 | Log in for price | ||