Biotage® SPE Dry

This application note describes a procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to clean up using ISOLUTE® SLE supported liquid extraction. The application note contains procedures optimized for 400 μL capacity column format and 200 μL and 400 μL supported liquid extraction plate formats, for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 ng/mL.
Tags: 6-MAM, 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Application Notes, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Hair, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lysergic acid diethylamide (LSD), MDA, MDMA, Mephedrone, Methadone, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Temazepam, Triazolam, UPLC-MS/MS, Zaleplone, Zolpidem, Zopiclone, α-OH alprazolam

This application note describes a streamlined procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to direct transfer to clean up using ISOLUTE® SLE supported liquid extraction.
Tags: 96-well plate, Alprazolam, Amphetamine, Application Notes, Bromazepam, Buprenorphine, Clinical, Clonazepam, Cocaine, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Fentanyl, Flunitrazepam, Flurazepam, Hair, Hydrocodone, Hydromorphone, Ketamine, Lorazepam, Lysergic acid diethylamide (LSD), MDA, MDEA, MDMA, Mephedrone, Methamphetamine, Methedrone, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Oxazepam, Oxycodone, Oxymorphone, PCB, Temazepam, Triazolam, UPLC-MS/MS, Zaleplone, Zolpidem, Zopiclone

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New, novel benzodiazepines have become some of the most abused unique psychoactive substances seen today. In the United States, these compounds are not approved for prescription use. Therefore, the demand for testing of these drugs has rapidly increased, both in the United States and worldwide. Urine and whole blood are common matrices of choice for forensic laboratories as they can be easy to collect, and they provide relevant information regarding recent or active use of illicit materials.
Tags: 96-well plate, Forensic, LC-MS/MS, Posters, Synthetic Benzodiazepines, Urine, Whole blood

Hydroxychloroquine (HCQ) and chloroquine (CQ) are common antimalarial drugs with long half-lives. They are currently being tested as potential treatments for COVID-19. Due to their high publicity in the media, many forensic toxicologists are working on developing methods to extract and detect these compounds in different matrices to understand the adverse effects, potential toxicity, and drug interactions associated with these drugs. These compounds are not typically included in general unknown screens. A simple sample preparation procedure that delivers clean extracts and analyte recovery greater than 90% for CQ and greater than 82% for HCQ with minimum matrix effects and high process efficiency was developed.
Tags: Biological fluids, Chloroquine (CQ), Clinical, Hydroxychloroquine (HCQ), LC-MS/MS, Posters

LC-MS/MS analysis of volatile analytes is problematic when 96-well plates are used. Solid phase extraction of urine with high drug concentrations can cause contamination of the elution solvent during evaporation as the compounds volatilize then redistribute in adjacent wells.This phenomenon, known as “evaporative crosstalk”, can generate false positive results.
Tags: Amphetamines, Forensic, LC-MS/MS, Methamphetamine, Posters

Routine testing for drugs of abuse (DOA) in urine is commonly performed by many clinical, forensic, and pain management laboratories. The method of testing varies but can often provide unwanted load stress for day-to-day operation in labs. Most seek simplified yet reliable and robust modes of sample preparation and analysis. ASMS, 2020.
Tags: 96-well plate, Amitriptyline, Clinical, Codeine, Drugs of Abuse, Hydromorphone, Lorazepam, Morphine, Naloxone, Norbuprenorphine, Oxazepam, Oxymorphone, Posters, THC-COOH, Urine

Evaporative crosstalk is well-to-well cross contamination during extract evaporation after SLE or SPE extraction in a 96 well plate. It is often observed with volatile analytes like methamphetamine and amphetamine, but it can also occur with other drugs and metabolites when urine samples have very high analyte concentrations. ASMS, 2020.
Tags: Amphetamine, Benzoylecgonine, Drugs of Abuse, Forensic, Hydromorphone, MDA, MDEA, MDMA, Methamphetamine, Morphine, Posters, Urine

Monitoring estrogenic compounds such as Estrone (E1), Estradiol (E2), and Estriol (E3) in human serum is essential in clinical research and diagnostics. Detection in men, post-menopausal women and children requires very low limits of detection (LODs) which can be challenging.
Tags: 96-well plate, Clinical, Estrogens, LC-MS/MS, Posters, Serum

Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing, SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
Tags: English, Product Notes