EVOLUTE® EXPRESS
Load-Wash-Elute SPE Columns & Plates
EVOLUTE® EXPRESS represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of SPE columns and 96-well plates combine powerful EVOLUTE sorbent chemistry with innovative features that enhance productivity. With improved flow-through characteristics and by eliminating the need for conditioning and equilibration, samples can be prepared much faster using the simplified Load-Wash-Elute procedure.
Fast & Efficient Flow Rates
EVOLUTE® EXPRESS products dramatically improve flow characteristics and enhance sample preparation productivity. Flow rates are fast and consistent even when loading aqueous biological fluid sample directly onto a dry column.
Reproducibility is Key
Specially treated inert, hydrophilic frits, water wettable polymeric EVOLUTE sorbents and the exact dimensions and packing techniques all work together to give you extremely consistent results, batch to batch.
No more Conditioning or Equilibration with the Load-Wash-Elute Procedure
Processing samples using EVOLUTE® EXPRESS columns or plates with a simplified Load-Wash-Elute procedure can increase productivity by eliminating the need for the conditioning and equilibration steps. This is made possible due to the combination of the water wettable EVOLUTE® sorbents and the aqueous compatible plate components incorporated in EVOLUTE® EXPRESS.
For most applications the Load-Wash-Elute procedure gives the same high analyte recoveries and low variability as standard SPE but can reduce processing time by one third.
STANDARD SPE Procedure |
EVOLUTE® EXPRESS Load-Wash-Elute Procedure |
| Condition | Not required |
| Equilibrate | Not required |
| Load | Load |
| Wash | Wash |
| Elute | Elute |
For All Your Analytes
EVOLUTE® EXPRESS ABN
- For simultaneous extraction of acidic, basic and/or neutral analytes Read More »
EVOLUTE® EXPRESS CX
- For extraction of basic analytes Read More »
EVOLUTE® EXPRESS WCX
- For extraction of strongly basic analytes Read More »
EVOLUTE® EXPRESS AX
- For extraction of acidic analytes Read More »
EVOLUTE® EXPRESS WAX
- For extraction of strongly acidic analytes Read More »
EVOLUTE® EXPRESS Sorbent Selection Plate
- EVOLUTE CX, WCX, AX and WAX in a single plate for sorbent screening and selection
EVOLUTE® polymer-based SPE sorbents extract neutral, acidic and basic analytes from biological fluids and other aqueous matrices, the EVOLUTE retention and elution characteristics are completely predictable and extractions on these robust sorbents are not affected by drying of the sorbent bed during sample processing.
Unlike other polymeric brands, EVOLUTE® EXPRESS combines a cleaner SPE sorbent with optimised components, making sample prep simple and more consistent from sample-to-sample.
Minimum Elution Volume-Maximum Efficiency
EVOLUTE® EXPRESS plates (10 mg format) can give full analyte recovery using elution volumes of 200 µL allowing direct injection of clean sample extracts. Analytical cycle time can be significantly reduced by removing the need to evaporate and reconstitute samples prior to analysis; this also eliminates the problem of analyte loss during this step.
Recovery profile of Ranitidine from plasma using EVOLUTE® EXPRESS CX 10 mg plate with generic extraction protocol
Method Development with EVOLUTE® EXPRESS Sorbent Selection Plate
EVOLUTE® EXPRESS Sorbent Selection Plate contains the four EVOLUTE® mixed-mode ion-exchange chemistries. It has been designed to provide a single plate for streamlined method development and identify the best solution to sample preparation challenges, for example extraction of unknowns or peptides.
Screening methods for the Sorbent Selection Plate based upon generic extraction protocols are illustrated in the schematic below. Once the best sorbent has been selected, extraction methodology can be optimized using the EVOLUTE® User Guide.
