EVOLUTE® EXPRESS ABN

This application note describes the extraction of a panel of estrogenic hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 5% for all analytes. Linearity of greater than 0.99 is achieved for Estrone (E1) and Estradiol (E2) from 5–2000 pg/mL, and 50–20000 pg/mL for Estriol (E3). No derivatization is required, and detection limits are enhanced using a fluorinated mobile phase.
Tags: 96-well plate, Application Notes, Clinical, English, Estradiol, Estriol, Estrogens, Estrone, LC-MS/MS, Serum, Steroid, Steroid Hormones

Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development. This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared. Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Anaesthetic, Anticonvulsant, Application Notes, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carbamates, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), EDDP, English, Fentanyl, Forensic, Gabapentin, Hallucinogen, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Noroxymorphone, Norpropoxyphene, Nortryptiline, O-desmethyltramadol, Opiates, Opioid, Opioid agonist, Oxazepam, Oxycodone, Oxymorphone, Pentobarbital, Phencyclidine, Phencyclidine (PCP), Phenobarbital, Pregabalin, Product Notes, Ritalinic acid, Secobarbital, Stimulants, Sympathomimetic amine, THC metabolites, THC-COOH, Tapentadol, Temazepam, Tramadol, Tricyclic Antidepressant, White Papers, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam

This application note describes the extraction of a panel of 19 steroid hormones from human urine using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC-MS/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 75% with RSDs lower than 5% for most analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 1–1000 pg/mL. Manual and automated (using Biotage Extrahera) extraction protocols are detailed.
Tags: 11-deoxycorticosterone, 17-OH-Pregnenolone, 17-alpha-estradiol, 17-alpha-hydroxyprogesterone, 17-beta-estradiol, 18-OH-Corticosterone, 21-deoxycortisol, 96-well plate, Aldosterone, Androstenedione, Application Notes, Clinical, Corticosterone, Cortisol, Cortisone, DHEA (dehydroepiandrosterone, DHEAS, Dihydrotestosterone (DHT), English, Estrone, Hormone, Hormones, LC-MS/MS, Pregnenolone, Progesterone, Steroid, Steroid Hormones, Steroid metabolite, Steroids, Testosterone, Urine

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL. The application note includes details on automation of the extraction method using Biotage® Extrahera.
Tags: 11-deoxycorticosterone, 11-deoxycortisol, 17-OH-Pregnenolone, 17-alpha-hydroxyprogesterone, 18-OH-Corticosterone, 21-deoxycortisol, 96-well plate, Aldosterone, Androstenedione, Application Notes, Clinical, Corticosterone, Cortisol, Cortisone, DHEA (dehydroepiandrosterone, DHEAS, Dehydroepiandrosterone (DHEA), Dihydrotestosterone (DHT), English, Estradiol, Estrogens, Estrone, Extrahera, Hormone, Hormones, LC-MS/MS, Pregnenolone, Progesterone, Serum, Steroid, Steroid Hormones, Steroid metabolite, Steroids

This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs in PRA determination.
Tags: 96-well plate, Aldosterone, Angiotensin I, Angiotensin II,, Application Notes, Clinical, English, Extrahera, LC-MS/MS, Peptides, Plasma, Steroid Hormones

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns. The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), Application Notes, Column, English, Extrahera, Forensic, GC-MS, Hydrolysed urine, THC metabolites, THC-COOH

This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application achieves high reproducible recoveries and excellent linearity (>0.99) in the range 10-200 ng/mL.
Tags: 5-hydroxyindoleacetic acid (5-HIAA) , 96-well plate, Acidic catecholamine metabolites, Application Notes, Clinical, English, Homovanillic acid (HVA), LC-MS/MS, Plasma, Vanillylmandelic acid (VMA)

Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.
Tags: Alcohol metabolite, Application Notes, Biomarker, Brochures, Catecholamines, Clinical, English, Hormone, Hormones, Pain Management, Peptides, Product Notes, Steroid, Steroid Hormones, Steroid metabolite, Steroids, Thyroid Hormone, Tobacco Specific Nitrosamines (TSNAs), Vitamin D, Vitamins, Water Soluble Vitamins

The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure. Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.
Tags: 96-well plate, Application Notes, Clinical, English, LC-MS/MS, Oxytocin, Peptides, Pharma, Serum, Vasopressin

The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.
Tags: 96-well plate, Application Notes, Biotin, Clinical, English, Extrahera, LC-MS/MS, Serum, Vitamin B7, Vitamins, Water Soluble Vitamins

This procedure is recommended for the extraction of a variety of diuretics from urine using non-polar polymeric SPE. The analyte suite includes thiazides, carbonic anhydrase inhibitors, loop and potassium sparring diuretics, with wide ranging pKa and logP values.
Tags: Acetazolamide, Amiloride, Application Notes, Bendoflumethiazide, Bumetanide, Clinical, Column, Diuretics, Ethacrynic acid, Furosemide, Hydrochlorothiazide, Hydroflumethiazide, LC-MS/MS, Methazolamide, Spironolactone, Urine

This procedure is recommended for the extraction of a variety of pharmaceuticals from water using non-polar polymeric SPE. These pharmaceuticals were identified as potential environmental contaminants by the Environment Agency. Pharmaceuticals, waste water, antibiotics, EVOLUTE,
Tags: Application Notes, Atenolol, Carbamazepine, Citalopram, Column, Diclofenac, Environmental, Erythromycin, Fluoxetine, Fluvoxamine, HPLC, Ibuprofen, Labetalol, Mefenamic, Metoprolol, Oxprenolol, Paroxetine, Pharmaceuticals, Propranolol, Sotalol, Sulfamethoxazole, Trimethoprim, Water

Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.
Tags: Alcohol metabolite, Application Notes, Biomarker, Brochures, Catecholamines, Clinical, English, Hormone, Hormones, Pain Management, Peptides, Product Notes, Steroid, Steroid Hormones, Steroid metabolite, Steroids, Thyroid Hormone, Tobacco Specific Nitrosamines (TSNAs), Vitamin D, Vitamins, Water Soluble Vitamins

In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing
Tags: Catalogs

Customer Case: Japan Frozen Foods Inspection Corporation. The Japan Frozen Foods Inspection Corporation (JFFIC) made the transition from traditional mouse testing of shellfish toxins, to instrumental analysis by developing original methods. In the sample preparation procedure JFFIC uses EVOLUTE® ABN hydrophilic/hydrophobic SPE columns from Biotage.
Tags: Customer Cases

EVOLUTE® EXPRESS Solid Phase Extraction products from Biotage are designed for all your sample preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic toxicology, or with the diversity of samples in food safety and environmental applications, the tools and methods used for sample preparation have a clear impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate, Clinical, Column, English, Environmental, Food, Forensic, Manuals & User Guides, On-line SPE, Pharma

QuickStart Guide to SPE
Tags: English, Manuals & User Guides

•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Tags: Manuals & User Guides

Tags: Manuals & User Guides

Laboratory chemical Not for therapeutic or diagnostic use
Tags: English, Material Safety Data Sheets

Laboratory chemical Not for therapeutic or diagnostic use
Tags: English, Material Safety Data Sheets

nvestigate different water wettable polymeric solid phase extraction sorbents for different analytes based analyte functional groups, pKa, and hydrophobicity, and reduce extraction time and increase throughput by eliminating SPE conditioning and equilibration steps.
Tags: 96-well plate, Amitriptyline, Atenolol, Bretylium, Clinical, Diclofenac, Ketoprofen, LC-MS/MS, Mefenamic acid, Metoprolol, Pharmaceuticals, Posters, Prednisolone, Quinidine, Sulindac, Urine

Compare and choose sample clean-up options for a panel of endogenous steroids in serum prior to quantitation by LC-MS based on ease of use, compounds of interest and desired limits of quantitation (LOQs).
Tags: 96-well plate, Forensic, LC-MS/MS, Posters, Serum, Steroid Hormones

GC/MS is still a mainstay in forensic analysis for drugs of abuse testing in urine. Historically silica-based solid phase extraction (SPE) columns have been used for these target analytes, the exact choice being dependent on drug functionality. The primary urinary target to prove cannabis using is the metabolite 11-nor-9-carboxy-Δ9 tetrahydrocannabinol.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 96-well plate, Drugs of Abuse, Forensic, GC-MS, Posters, Urine

GC/MS is still a mainstay in forensic analysis for drugs of abuse testing in urine. Historically silica-based solid phase extraction (SPE) columns have been used for these target analytes, the exact choice being dependent on drug functionality. This poster aims to compare various sample preparation techniques for this analysis. ASMS, 2020.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 96-well plate, Drugs of Abuse, Forensic, GC-MS, Posters, Urine

Monitoring estrogenic compounds such as Estrone (E1), Estradiol (E2), and Estriol (E3) in human serum is essential in clinical research and diagnostics. Detection in men, post-menopausal women and children requires very low limits of detection (LODs) which can be challenging.
Tags: 96-well plate, Clinical, Estrogens, LC-MS/MS, Posters, Serum

Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. MSACL EU 2018 MSACL NA 2019
Tags: 96-well plate, Aldosterone, Angiotensin I, Angiotensin II,, Clinical, English, Hormone, Hormones, LC-MS/MS, Peptides, Posters, Steroid, Steroid Hormones, Steroids

This poster compares sample preparation options for the extraction of a panel of endogenous steroids from serum. LC-MS/MS parameters were investigated for increased sensitivity: MRM transitions, chromatography and mobile phase additives for use with positive and negative ionisation modes. Particular emphasis was placed on the sample preparation to provide high reproducible recoveries whilst minimizing matrix effects and co-extracted materials such as proteins and phospholipids. Solid phase extraction was compared to supported liquid extraction in terms of recoveries, ion suppression, phospholipid content, calibration curve performance and overall sensitivity. MSACL EU 2018
Tags: 96-well plate, Clinical, English, Hormone, Hormones, LC-MS/MS, Posters, Steroid, Steroid Hormones, Steroid metabolite, Steroids

Urine drug testing to support pain management is a mainstay of the clinical toxicology laboratory. Reduced reimbursement has continued to put increasing pressure on laboratories. Many toxicology labs are moving to larger drug panels to increase throughput and efficiency, while reducing turn-around-time and cost.
Tags: Clinical, LC-MS/MS, Pharma, Posters

This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels. Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc). ASMS 2017
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Analgesics, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Fentanyl, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Nortryptiline, Opiates, Opioid, Oxazepam, Oxycodone, Oxymorphone, Pain Management, Phencyclidine, Phenobarbital, Posters, Pregabalin, Ritalinic acid, Secobarbital, Tapentadol, Temazepam, Tramadol, Urine, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam

This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection. ASMS 2017
Tags: 96-well plate, Clinical, English, Oxytocin, Peptides, Pharma, Posters, Serum, Vasopressin

Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize sensitivity allowing low level analysis of vitamin B7 from human serum. MSACL 2017, Palm Springs ASMS 2017, Indianapolis
Tags: 96-well plate, Biotin, Clinical, English, LC-MS/MS, Posters, Serum, Vitamin B7, Vitamins, Water Soluble Vitamins

This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples. The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060). ASMS 2017
Tags: 96-well plate, Amikacin, Aminoglycoside antibiotics, Apramycin, Dihydrostreptomycin, Eggs, English, Extrahera, Food, Gentamicin, Gentamicin C1, Gentamicin C1a, Gentamicin C2, Hygromycin B, Kanamycin, LC-MS/MS, Meat, Milk, Neomycin, Netilmicin, Paromomycin, Posters, Sisomycin, Spectinomycin, Streptomycin, Tobramycin, Veterinary drugs

This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX). ASMS 2017
Tags: Ciguatoxin 3C (CTX3C), Column, Diarrhetic Shellfish Poisoning (DSP) Toxins, Dinophysistoxin 1 (DTX1), Dinophysistoxin 2 (DTX2), English, Food, LC-MS/MS, Marine Toxins, Okadaic Acid (OA), Paralytic Shellfish Poisoning (PSP) Toxins, Posters, Tetrodotoxin (TTX)

EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required. ASMS 2015
Tags: Acidic, Amitriptyline, Atenolol, Benzenesulfonic acid, Bretylium, Clinical, Column, Diethyldithiophosphate (DEDTP), Dimethylthiophosphate (DMTP), English, Extrahera, Forensic, Hexanesulfonic acid, Ketoprofen, LC-MS/MS, Metoprolol, Mianserin, Organophosphate Pesticide, Over the counter pharmaceuticals, Pain Management, Pharma, Pharmaceuticals, Posters, Prednisolone, Procainamide, Quaternary Amines, Quinidine, Salbutamol, Sulindac, Tricyclic Antidepressant, Urine, β-blockers

This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts. HPLC 2014
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters

This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches. HPLC2014
Tags: Biological fluids, English, HPLC, LC-MS/MS, Plasma, Posters

This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices. Pittcon 2014
Tags: 96-well plate, ACTH (1-24), Angiotensin Fragment 1-7, Angiotensin II,, Clinical, Elafin, Endorphin, English, Glycoprotein myelin, Helicobacter pylori (2-20), LC-MS/MS, Peptides, Pharma, Plasma, Posters, Serum, Vasoactive Intestinal Fragment 1-12

This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012. EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24), Alpha-Endorphin, Angiotensin Fragment 1-7, Angiotensin II,, Elafin, Helicobacter pylori (2-20), LC-MS/MS, Myelin Glycoprotein fragment (35-55) rat, Peptides, Pharma, Plasma, Posters, Vasoactive Intestinal Fragment 1-12

Tags: Posters

Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development. This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared. Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Anaesthetic, Anticonvulsant, Application Notes, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carbamates, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), EDDP, English, Fentanyl, Forensic, Gabapentin, Hallucinogen, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Noroxymorphone, Norpropoxyphene, Nortryptiline, O-desmethyltramadol, Opiates, Opioid, Opioid agonist, Oxazepam, Oxycodone, Oxymorphone, Pentobarbital, Phencyclidine, Phencyclidine (PCP), Phenobarbital, Pregabalin, Product Notes, Ritalinic acid, Secobarbital, Stimulants, Sympathomimetic amine, THC metabolites, THC-COOH, Tapentadol, Temazepam, Tramadol, Tricyclic Antidepressant, White Papers, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam

Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.
Tags: Alcohol metabolite, Application Notes, Biomarker, Brochures, Catecholamines, Clinical, English, Hormone, Hormones, Pain Management, Peptides, Product Notes, Steroid, Steroid Hormones, Steroid metabolite, Steroids, Thyroid Hormone, Tobacco Specific Nitrosamines (TSNAs), Vitamin D, Vitamins, Water Soluble Vitamins

Tags: Column, English, Product Notes

In diesem Whitepaper sollen verschiedene Aspekte der Probenvorbereitung beleuchtet werden. Dazu gehört auch der Einfluss der Probenvorbereitung auf den Erfolg der nachfolgenden LC-MS/MS-Analyse von breit angelegten Urin-Panels.
Tags: White Papers

Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development. This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared. Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH), 6-acetylmorphine (MAM), 7-Aminoclonazepam, 96-well plate, Alprazolam, Amitriptyline, Amphetamine, Amphetamines, Anaesthetic, Anticonvulsant, Application Notes, Barbiturates, Benzodiazepines, Benzoylecgonine, Buprenorphine, Butalbital, Cannabinoid, Carbamate hypnotic, Carbamate muscle relaxant, Carbamates, Carisoprodol, Chlordiazepoxide, Clinical, Clonazepam, Cocaine, Cocaines, Codeine, Diazepam, Dihydrocodeine (DHC), EDDP, English, Fentanyl, Forensic, Gabapentin, Hallucinogen, Hydrocodone, Hydrolysed urine, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDMA, Meperidine, Meprobamate, Methadone, Methamphetamine, Morphine, N-des-tapentadol, Naloxone, Norbuprenorphine, Nordiazepam, Norfentanyl, Norhydrocodone, Norketamine, Normeperidine, Noroxymorphone, Norpropoxyphene, Nortryptiline, O-desmethyltramadol, Opiates, Opioid, Opioid agonist, Oxazepam, Oxycodone, Oxymorphone, Pentobarbital, Phencyclidine, Phencyclidine (PCP), Phenobarbital, Pregabalin, Product Notes, Ritalinic acid, Secobarbital, Stimulants, Sympathomimetic amine, THC metabolites, THC-COOH, Tapentadol, Temazepam, Tramadol, Tricyclic Antidepressant, White Papers, Zolpidem, Zolpidem-phenyl-4-COOH, z-drugs, α-OH alprazolam