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EVOLUTE® EXPRESS ABN On-line SPE Cartridges
EVOLUTE® EXPRESS ABN is a high purity, polymeric sorbent suitable for extraction of a broad range of acidic, basic and neutral analytes. EVOLUTE® EXPRESS ABN is now available in on-line cartridge format. Download the product note.
Typical Applications:
- Pharmaceuticals
- Personal care products
- General organics
- Acids, bases and neutrals
An optimized combination of non-polar (hydrophobic) and polar (hydrophilic) interactions result in a versatile sorbent for extraction of a broad range of compounds encountered in routine environmental analysis. Because EVOLUTE EXPRESS ABN is totally water wettable, it will never become de-conditioned, even if it runs dry. It does not need to be stored in solvent after use, and is a great alternative to C18 and PLRP type materials.
Structure of EVOLUTE® EXPRESS ABN sorbent
Biotage EVOLUTE EXPRESS ABN on-line cartridges combine these unique sorbent characteristics with the convenience of fully automated SPE.
Sorbents and columns are fully QC tested to ensure excellent performance and reproducibility.
Biotage on-line SPE columns can be used with most commercially available on-line SPE systems.
See technical note PPS424 for more information.
| Nominal particle size | 20 µm |
| Pore size | 40 Å |
| Surface area | 400 m2/g |
| pH stability | 1-14 |
» APPLICATION NOTES
Analysis of Pharmaceuticals in Water Using Automated On-line SPE-LC-MS/MS
This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.
Tags: Application Notes,
Atenolol,
Atorvastatin,
Azithomycin,
Carbamazepine,
Ciprofloxacin,
Clarithromycin,
Diclofenac,
English,
Environmental,
Erythromycin,
Fluoxetine,
On-line SPE,
On-line SPE-LC-MS/MS,
Pharmaceuticals,
Prednisolone,
Ranitidine,
Sertraline,
Tolytriazole,
Water
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags: Catalogs
» MANUALS & USER GUIDES
EVOLUTE® EXPRESS User Guide
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate,
Clinical,
Column,
English,
Environmental,
Food,
Forensic,
Manuals & User Guides,
On-line SPE,
Pharma
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
» POSTERS
Sample Preparation Strategies for Urine Panels with 50 or More Drugs and Metabolites Analyzed by LC-MS/MS
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
ASMS 2017
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH),
6-acetylmorphine (MAM),
7-Aminoclonazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Buprenorphine,
Butalbital,
Cannabinoid,
Carbamate hypnotic,
Carbamate muscle relaxant,
Carisoprodol,
Chlordiazepoxide,
Clinical,
Clonazepam,
Cocaine,
Cocaines,
Codeine,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Morphine,
N-des-tapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Nortryptiline,
Opiates,
Opioid,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Phencyclidine,
Phenobarbital,
Posters,
Pregabalin,
Ritalinic acid,
Secobarbital,
Tapentadol,
Temazepam,
Tramadol,
Urine,
Zolpidem,
Zolpidem-phenyl-4-COOH,
z-drugs,
α-OH alprazolam
Method Development Strategies Using Polymer-based SPE for the Analysis of Peptides Prior to UPLC-MS/MS Analysis
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
ASMS 2017
Tags: 96-well plate,
Clinical,
English,
Oxytocin,
Peptides,
Pharma,
Posters,
Serum,
Vasopressin
Sensitive and Fast Measurement of Aminoglycoside Antibiotics in Milk, Meat or Eggs by HILIC-MS/MS and Identifcation using MRM Spectrum Mode
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
ASMS 2017
Tags: 96-well plate,
Amikacin,
Aminoglycoside antibiotics,
Apramycin,
Dihydrostreptomycin,
Eggs,
English,
Extrahera,
Food,
Gentamicin,
Gentamicin C1,
Gentamicin C1a,
Gentamicin C2,
Hygromycin B,
Kanamycin,
LC-MS/MS,
Meat,
Milk,
Neomycin,
Netilmicin,
Paromomycin,
Posters,
Sisomycin,
Spectinomycin,
Streptomycin,
Tobramycin,
Veterinary drugs
Catecholamine Analysis: Evaluation of Method Optimization to Improve Sensitivity and reduce Limits of Quantitation using LC-MS/MS
This poster examines various factors in the development and optimisation of a SPE-LC-MS/MS method for extraction of catecholamines from plasma.
The final method utilizes EVOLUTE EXPRESS WCX plates in either standard SPE or fast Load-wash-elute modes, and incorporates a series of wash steps to optimise analytical sensitivity. Low elution volumes mean no evaporation step is required, and LLOQ is 20mg/mL or lower for each of the analytes.
Tags: 96-well plate,
Catecholamines,
Clinical,
Dopamine,
English,
Epinephrine,
LC-MS/MS,
Norepinephrine,
Plasma,
Posters
Effective Strategies for Phospholipid Removal using Polymer-based Solid Phase Extraction (SPE) and LC-MS/MS Analysis
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
HPLC 2014
Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters
Sample Preparation and Protein: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel Electrophoresis
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
HPLC2014
Tags: Biological fluids,
English,
HPLC,
LC-MS/MS,
Plasma,
Posters
Fast and Clean Extraction of Peptide Mixture from Biological Matrices with no Dry Down Steps Using EVOLUTE® ABN and EVOLUTE® EXPRESS ABN Solid Phase Extraction Prior To Liquid Chromatography Tandem Mass Spectrometry
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Pittcon 2014
Tags: 96-well plate,
ACTH (1-24),
Angiotensin Fragment 1-7,
Angiotensin II,,
Clinical,
Elafin,
Endorphin,
English,
Glycoprotein myelin,
Helicobacter pylori (2-20),
LC-MS/MS,
Peptides,
Pharma,
Plasma,
Posters,
Serum,
Vasoactive Intestinal Fragment 1-12
Fast and Clean Extraction of Peptide Mixture from Biological Matrices with no Dry Down Steps Using EVOLUTE® ABN and EVOLUTE® EXPRESS ABN Solid Phase Extraction Prior To Liquid Chromatography Tandem Mass Spectrometry
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24),
Alpha-Endorphin,
Angiotensin Fragment 1-7,
Angiotensin II,,
Elafin,
Helicobacter pylori (2-20),
LC-MS/MS,
Myelin Glycoprotein fragment (35-55) rat,
Peptides,
Pharma,
Plasma,
Posters,
Vasoactive Intestinal Fragment 1-12
» PRODUCT NOTES
EVOLUTE® EXPRESS ABN On-line SPE Cartridges
Product Note: EVOLUTE® EXPRESS ABN On-line SPE Cartridges
Tags: English,
Environmental,
LC-MS/MS,
On-line SPE,
Product Notes
Search all documents »
News
24 October 2017Extraction of Desmopressin from Serum Using EVOLUTE® EXPRESS WCX Prior to LC-MS/MS Analysis
This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...
05 July 2017Extraction of Plasma Catecholamines and Metanephrines Using EVOLUTE® EXPRESS WCX SPE Prior to LC-MS/MS Analysis
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...
29 May 2017Extraction of Polyfluorinated Compounds in Water Using EVOLUTE® EXPRESS WAX SPE Prior to LC-MS/MS Analysis
Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...
19 May 2017Extraction of Oxytocin and Vasopressin from Serum Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...
20 April 2017Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...
25 July 2016Fully Automated Analysis of Pharmaceuticals in Water
This new application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescrib...
07 June 2016New On-line SPE Cartridges from Biotage
Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the launch of a new range of On-line SPE Cartridge...
15 October 2015Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
10 July 2014New Posters by Biotage at ASMS 2014, Baltimore, MD, USA
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...
30 June 2014EPRW (European Pesticides and Residues Workshop) 2014, Dublin
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/
20 May 2014New Posters by Biotage R&D Presented at HPLC 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...
01 April 2014Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT), Hannover
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...
11 January 201422nd Congress French Toxicology Society 19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society, Bordeuax
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...
28 August 2012Biotage announces EVOLUTE® EXPRESS 96-well plates
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...
28 August 2012Biotage unveils EVOLUTE® EXPRESS
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...
18 April 2011Biotage® Introduces EVOLUTE® WAX, Providing a Total Solution for Acidic Compounds
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...
16 December 2010Biotage introduces a new sample preparation application note for the extraction of ethyl glucuronide from urine by LC-MS
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...
23 February 2010Biotage Introduces EVOLUTE® WCX: Now Providing a Total Solution for Basic Compounds
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Q&A
Expiry date on ISOLUTE and EVOLUTE
01 July 2013Question:
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
Answer:
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
Recommended storage conditions
- Always store products sealed inside metal laminate barrier bags.
- Store products at room temperature, away from direct sunlight.
- Ensure products are not in contact with moisture, direct heat etc.
Non-bonded phases (e.g. Si, Fl, Al-A/N/B)
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
Bonded phases (e.g. C18, SCX, DIOL)
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
Browse more questions and answers >CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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OSPE-620-32150 | EVOLUTE® EXPRESS ABN On-line SPE cartridge 30 x 2.1 mm | Consumables | 1 | Log in for price | |