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EVOLUTE® EXPRESS WCX

Advanced Polymeric SPE for Strong Bases

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612-0015-cxg 612-0015-cxg 612-0006-bx

EVOLUTE® EXPRESS Sample Preparation products provide robust, reliable SPE, giving ultra pure extracts and high analyte recoveries. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE sorbents provide a highly effective solution to the problems of ion suppression and matrix effects. 

Eliminate conditioning and equilibration with EVOLUTE EXPRESS load-wash-elute SPE.

CONSUMABLES

Part No. Description Product type Pack Size Price
602-0001-AXG 602-0001-AXG EVOLUTE® EXPRESS WCX 10 mg/1 mL (Tabless) Consumables 100 Log in for price
602-0003-AXG 602-0003-AXG EVOLUTE® EXPRESS WCX 30 mg/1 mL (Tabless) Consumables 100 Log in for price
602-0010-PX01 602-0010-PX01 EVOLUTE® EXPRESS WCX 10 mg Fixed Well Plate Consumables 1 Log in for price
602-0030-PX01 602-0030-PX01 EVOLUTE® EXPRESS WCX 30 mg Fixed Well Plate Consumables 1 Log in for price
612-0006-BXG 612-0006-BXG EVOLUTE® EXPRESS WCX 60 mg/3 mL (Tabless) Consumables 50 Log in for price
612-0006-BXG-1 612-0010-BXG EVOLUTE® EXPRESS WCX 100 mg/3 mL (Tabless) Consumables 50 Log in for price
612-0015-cxg 612-0015-CXG EVOLUTE® EXPRESS WCX 150 mg/6 mL (Tabless) Consumables 30 Log in for price
612-0050-CXG 612-0050-CXG EVOLUTE® EXPRESS WCX 500 mg/6 mL (Tabless) Consumables 30 Log in for price
650-0010-PX01 650-0010-PX01 EVOLUTE® EXPRESS SORBENT SELECTION 10 mg Fixed Well Plate Consumables 1 Log in for price
650-0030-PX01 650-0030-PX01 EVOLUTE® EXPRESS SORBENT SELECTION 30 mg Fixed Well Plate Consumables 1 Log in for price

ACCESSORIES

Part No. Description Product type Pack Size Price
121-9620 121-9620 Tabless 1 mL Column Holder, VacMaster™-96 Accessories 1 Log in for price
PPM-A96-CH PPM-A96-CH 1 mL (Tabless) Column Holder PRESSURE+ 96 Accessories 1 Log in for price

EVOLUTE® WCX combines non-polar and weak cation exchange functionality for extraction of strongly basic analytes (e.g. quaternary amines) from biological fluids and other aqueous samples through a dual, mixed-mode mechanism. This provides additional selectivity and clean-up for complex samples, with salts, phospholipids and proteins eliminated from extracts. Available in a variety of formats, including modular and 96-well plates and SPE columns, and two different particle sizes (30 µm and 50 µm) depending on application requirements. Sorbent performance is not compromised by drying during processing.

The sorbent consists of the EVOLUTE® backbone, surface modified with carboxylic acid groups.

Figure 1. Structure of EVOLUTE WCX sorbent

EVOLUTE® WCX sorbent is available in EVOLUTE® EXPRESS SPE columns and  96-well plate formats for use with fast Load-Wash-Elute procedures. Eliminate traditional conditioning and equilibration and increase throughput without compromising performance.

High Selectivity Provides Ultra-clean Extracts

EVOLUTE sorbents have a narrow pore diameter, tailored to eliminate co-extraction of proteins from biofluids. Removal of proteins during sample preparation avoids transfer in to the analytical system, reducing matrix effects in LC-MS/MS, and avoiding contamination of the analytical column. This minimises the need for frequent backflushing and guard columns replacement.

Using EVOLUTE® WCX, interferences including proteins, salts, non-ionisable molecules and phospholipids are removed during the interference elution step, ensuring extremely clean extracts.

                

The combination of optimized pore structure and additional interference elution steps using mixed-mode sorbent chemistry guarantees extremely clean extracts.

High Analyte Recoveries

EVOLUTE® WCX uses a generic procedure for extraction of strongly basic analytes in aqueous matrices. The dual retention mechanism retains strong acids through a combination of non-polar and weak cation exchange interactions. The simple wash steps in the generic method remove >98% of phospholipids from plasma samples.

The combination of robust polymer chemistry, uniform coverage of carboxylic acid groups and optimum exchange capacity provide greater than 85 % recovery for strongly basic analytes such as quaternary amines.

See the EVOLUTE User Guide for a wealth of method optimisation information.

Excellent Flow Characteristics

Narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channelling, which can lead to analyte breakthrough. 

Even viscous biofluids can be loaded onto EVOLUTE EXPRESS WCX products without the need for conditioning and equilibration, using fast load-wash-elute SPE.

Batch-to-batch reproducibility

The surface characteristics of each batch of EVOLUTE® WCX are quality controlled and tested to ensure consistency.

  • High, reproducible recoveries for strongly basic analytes
  • Eliminate salts, phospholipids and proteins from biofluid extracts, and minimise ion suppression
  • Fast simple generic methodology
  • Available in EVOLUTE EXPRESS SPE columns and plates for fast  Load-Wash-Elute procedures