
This product was withdrawn on April 1st 2015. It been replaced by the EVOLUTE® EXPRESS product line.
Chemical description | Primary/secondary amine modified polystyrene-divinylbenzene incorporating non-ionizable hydroxyl groups |
Sorbent type | Mixed-mode non-polar/weak anion exchange |
Exchange capacity | 0.3 mmol/g |
Average particle size | 30 µm, 50 µm |
Pore diameter | 40 Å |
pKa | ~10 |
This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL.
resin, polymer, EVOLUTE,
Tags: Application Notes,
Biomarker,
Clinical,
Column,
Diethyldithiophosphate (DEDTP),
Diethylphosphate (DEP),
Diethylthiophosphate (DETP),
Dimethyldithiophosphate (DMDTP),
Dimethylphosphate (DMP),
Dimethylthiophosphate (DMTP),
LC-MS/MS,
Organophosphate Pesticide,
Urine
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
HPLC2014
Tags: Biological fluids,
English,
HPLC,
LC-MS/MS,
Plasma,
Posters
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags: 96-well plate,
Alcohol metabolite,
Column,
Ethyl Sulfate (EtS),
Ethyl glucuronide (EtG),
Forensic,
LC-MS/MS,
Posters,
Urine
EVOLUTE® WAX combines non-polar and weak anion exchange functionality for extraction of strongly acidic analytes from biological fluids and other aqueous samples through a dual, mixed-mode mechanism. This provides additional selectivity and clean-up for complex samples, with salts, phospholipids and proteins eliminated from extracts. Available in a variety of formats, including modular and 96-well plates and SPE columns, and two different particle sizes (30 µm and 50 µm) depending on application requirements.
The sorbent consists of the EVOLUTE backbone, surface modified with primary/secondary amine groups.
Figure 1. Structure of EVOLUTE® WAX sorbent
EVOLUTE® WAX sorbent is available in EVOLUTE® EXPRESS 96-well plate format for use with fast Load-Wash-Elute procedures. Eliminate traditional conditioning and equilibration and increase throughput without compromising performance.
EVOLUTE sorbents have a narrow pore diameter, tailored to eliminate co-extraction of proteins from biofluids. Removal of proteins during sample preparation avoids transfer in to the analytical system, reducing matrix effects in LC-MS/MS, and avoiding contamination of the analytical column. This minimises the need for frequent backflushing and guard columns replacement.
Using EVOLUTE WAX, interferences including proteins, salts, non-ionisable molecules and phospholipids are removed during the interference elution step, ensuring extremely clean extracts (figure 2).
Figure 2. The combination of optimized pore structure and additional interference elution steps using mixed-mode sorbent chemistry guarantees extremely clean extracts.
EVOLUTE® WAX uses a generic procedure for extraction of strongly acidic analytes in aqueous matrices. The dual retention mechanism retains strong acids through a combination of non-polar and weak anion exchange interactions. The simple wash steps in the generic method remove >98% of phospholipids from plasma samples.
The combination of robust polymer chemistry, uniform coverage of secondary amine groups and optimum exchange capacity provide greater than 85 % recovery for a wide range of strongly acidic analytes.
See the EVOLUTE User Guide for a wealth of method optimisation information.
Figure 2. Recovery of strongly acidic analytes from urine samples using the EVOLUTE WAX 25 mg fixed well plate. Analytes: dimethylthiophosphate (DMTP), diethylphosphate (DEP), hexanesulfonic acid (HSA), benzenesulfonic acid (BSA).
Narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channelling, which can lead to analyte breakthrough. Additionally, larger particle EVOLUTE® WAX 50 µm allows for increased flow rates when using particularly viscous samples or large sample volumes.
The surface characteristics of each batch of EVOLUTE® WAX are quality controlled and tested to ensure consistency.