
Isolera™ Dalton is the most advanced and innovative flash purification system available, designed by people who understand flash chromatography for the chemists who need it.
"Pure convenience! Just pure convenience! It lets us perform purification and analysis at the same time. It gives us tremendous power to isolate unknown compounds.”
- Hiroki Nishii, Chugai Pharmaceutical’s Kamakura Research Laboratories
Dimensions (W x H x D) | Isolera™ Dalton Mass Detector: 35.5 x 56 x 25 cm Isolera™ Dalton Nanolink: 35.4 x 52.5 x 21 cm Isolera™ Dalton System: 85 x 59 x 62 cm |
Weight | Isolera™ Dalton Mass Detector: 32 kg Isolera™ Dalton Nanolink: 10 kg Isolera™: 35 kg Isolera™ Dalton System: 77 kg |
Mass Analyzer | Miniaturized single quadrupole |
Ionization Source | Chip based electrospray ionization |
Mass Accuracy | ± m/z 0.5 in full scan in a temperature controlled environment, ±3 °C |
Sampling Flash to MS | Automatic |
Start-up Time | Initially 30 mins from off state |
Vacuum Pumps | On board and fully integrated |
Control Interface | Isolera™ Spektra |
This application note describes optimization for a fully automated synthesis and on-resin cyclization of oxytocin enabled by Branches™, a unique software feature of the Biotage® Initiator+ Alstra™ peptide synthesizer.
Tags: Application Notes
This work demonstrates that peptide purification efficiency by flash
chromatography is improved with Biotage® SNAP Bio cartridges containing a
spherical wide pore stationary under a wide variety of purification conditions.
Tags: Application Notes
Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.
Tags: Application Notes
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Tags: Application Notes
The term “Green Chemistry” has become a major part of the
science community’s lexicon. In this application note we will
look at two areas for flash chromatography:
1. Replacing chlorinated solvents with those considered more
environmentally friendly.
2. Reducing solvent use and waste generation with more
thoughtfully applied chromatography principles.
Tags: Application Notes
In mass detection, the presence of ions can suppress peaks
from the analytes of interest. This study concludes that
Isolera™ Dalton performs successful separations even with
ions present in the sample.
Tags: Application Notes
Fragrance compounds, being invisible to UV, can be
elusive and difficult to isolate. In this application, a four
component mixture of fragrance compounds was purified
using Accelerated Chromatographic Isolation™ (ACI) and
identified by mass detection.
Tags: Application Notes
This application note shows how the two
closely related peptide structures (MW 2139 and 2267) were
separated using reversed phase mass directed flash chromatography
with Isolera™ Dalton.
Tags: Application Notes
Mass-directed flash purification is a simple and effective
technique to detect and fractionate compounds with poor UV chromophores. In this study, fatty acid methyl ester mixtures were purified by mass-directed flash purification using the Biotage Isolera™ Dalton System.
Tags: Application Notes
Traditional RP-HPLC techniques not always critical because improvements in solid-phase peptide synthesis techniques over the years (microwave heating and ChemMatrix® solid phase supports) have reduced the burden on downstream purification steps. Mass directed flash purification can be a viable alternative to more expensive (and lower capacity) semi- or prep-scale RP-HPLC methods.
Tags: Application Notes
In this application note we demonstrate the power of mass detection in Isolera™ Dalton by investigating the incomplete separation of two dyes, Butter yellow (Mass 225.29 g/mol) and Sudan red (Mass 278.31 g/mol), by normal phase flash purification.
Tags: Application Notes,
English
Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.
Tags: Application Notes,
English
In this example, Isolera™ Dalton was used to fractionate completely non-UV absorbing starting materials and products from a classic reductive amination reaction. A resin bound cyanborohydride reagent was used to greatly facilitate the work flow by eliminating the need for traditional work-up, and the mixture applied to flash chromatography purification methods.
Isolera, Dalton
Tags: Application Notes,
English
Isolera™ Dalton was used to fractionate closely running nicotinic extracts from commercially available tobacco leaves.
Keywords: natural, products, normal, extraction, tobacco, nicotine, nornicotine
Tags: Application Notes,
English
Be sure you have your target compound the moment your purification is complete. The Isolera™
Dalton integrates the Biotage approach to flash chromatography with true compound identification
using mass detection.
Tags: Brochures
User report: Flash instruments. Chugai Pharmaceutical uses Biotage flash chromatography products for drug discovery research. When deciding to convert from manual open-column procedures to automated systems, they chose successive generations of Biotage products, ranging from the Flash+® packed column to the Biotage® Horizon, SP1, Isolera™ Spektra, and Isolera™ Dalton automated flash chromatography systems.
Tags: Customer Cases
User Report: Isolera™ Dalton, Okayama University. The laboratory of Prof. Hiroyuki Miyachi at Okayama University installed Biotage’s Isolera Dalton, an automated mass-directed purification system for flash chromatography, allowing the fractionation and collection of optical isomers. “I felt like the world’s first mass-detection automated purification system had finally arrived.”
Tags: Customer Cases
Leu-Enkephalin-amide (YGGFLNH2, Ca. MW = 554.65) was synthesized on the Rink amide ChemMatrix® resin at a scale of 0.5 mmol. Crude peptide (100 mg) was purified in duplicate using the Isolera Dalton equipped with a Biotage® SNAP KP-C18-HS 12g cartridge.
Tags: Customer Cases,
English
The direct injection kit is intended to be used to inject samples into Isolera™ Dalton Nanolink using a syringe with a Luer-Lok tip.
Tags: English,
Manuals & User Guides
If high back pressure is detected in the makeup pump lines, and/or the High Makeup Flow Pressure dialog appears, the microfilter may be partially clogged and needs replacing. This is a step by step guide.
Tags: Manuals & User Guides
Isolera™ Dalton System Care Guide
Tags: English,
Manuals & User Guides
Recently there has been substantial motivation to consider and evaluate alternative, more environmentally friendly solvents. Some countries have even gone so far as to ban some of the more toxic, yet commonly used solvents. In addition to general toxicity, the volume of solvent used in any particular application is given extra consideration in the green chemistry movement. In this regard, purification solvent selection is closely monitored as they are often
used in large quantities.
Tags: English,
Environmental,
Posters
Peptide purification using standard reversed phase HPLC methods are hampered by low loading capacity, resulting in purifications that demand significant time investment. Recently, the use of reversed phase flash chromatography has increased in popularity for peptide purification due to the significant reduction of purification time,
enabled by the increased loading levels of the larger stationary phase particles. Resolution, though, is somewhat diminished with the larger particle size, demanding creative techniques to retain a highly pure peptide product.
Tags: Posters
In medicinal chemistry, organic synthesis will generate by-products with similar chemistry to the product. Separating these impurities from the product often becomes a challenge that often only prep HPLC can achieve.
In this poster, we discuss how using sample dry loading with a cation exchange scavenger media with flash chromatography
improves product purity by removing many of the impurities.
Tags: Posters
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
Tags: English,
Posters
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Tags: Posters
In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids.
Presented at ACS Spring 2014.
Tags: Posters
The tasks carried out during the maintenance visit shall include
servicing the listed components and a final check which is
based on factory acceptance and qualifications tests. These
two procedures constitute a Biotage Preventive Maintenance
(PM) Plan.
Tags: Preventive Maintenance Checklist
This document outlines service products specific to Isolera™ Dalton.
For further information on Biotage service products please see
“Biotage Service Agreements” brochure (PPS330).
Tags: Product Notes
The mass-directed flash purification system Isolera™ Dalton produces its final mass spectrum by compiling data from hundreds of individual mass spectra in real time.
Tags: Product Notes
Isolera Dalton identifies compounds by mass in real time during flash separation, leading to greater confidence in purification, higher purity, greater recovery, and a significant saving in time. Reduce or eliminate the need for time consuming off-line mass analysis during development programs, increase throughput and effectiveness for both chemists and analytical groups.
Tags: Product Notes
Use this simple tool to look up possible adducts and fragments which may occur with Isolera™ Dalton or Biotage® Dalton 2000 mass detectors.
Enter the target compound weight and use the mass lookup cell to highlight resulting m/z values.
Tags: Software
In-line mass detection has become a powerful addition to the flash chemist's toolbox. While this technical advance in flash chromatography has helped chemists detect and isolate pure compounds...
13 July 2017Biotage is pleased to announce the release of the new Peptide Synthesis Workflow – Synthesis, Purification, and Evaporation Solutions Brochure The typical peptide synthesis workflow broadly ...
24 February 2017"The use of a mass detector for flash chromatography detection provides enormous value as it confirms that the target compound has been isolated and that it has been identified and fractionated as ...
13 December 2016This webinar discusses some recent work on the use of flash chromatography for the purification of synthetic peptides as a complementary alternative to standard RP-HPLC methods. Included herein are...
01 November 2016Results of an orthogonal flash purification approach using normal-phase followed by reversed-phase flash chromatography of a cannabis extract is reported in a poster from Biotage, presented at ACS ...
04 February 2016This application note demonstrates how to successfully purify a low UV-absorbing carbohydrate derivative using the mass-directed flash chromatography system Isolera™ Dalton from Biotage....
01 February 2016Dr. Patrick Gunning and his research team at the University of Toronto, Canada, are on the verge of a breakthrough in developing a less harmful treatment method for blood cancer. Their laboratory i...
18 January 2016The use of fast and precise microwave heating in solid phase peptide synthesis can result in peptides of greater purity, thereby simplifying the subsequent purification steps. In this application n...
30 September 2015The vac-chip™ on Isolera™ Dalton is a vacuum interface. It allows the mass detector to achieve the operating pressures required for analysis and it aligns the ions from the sp...
22 September 2015The spraychip® on Isolera™ Dalton is a factory-calibrated disposable nanospray interface. The spraychip may become blocked or dirty over time and it may need to be cleaned ...
24 April 2015Japanese chemical detectives have used Isolera™ Dalton mass-driven flash chromatography system and Biotage® SNAP Ultra flash cartridges to identify the origins of traditional medicin...
16 March 2015Professor Hiroyuki Miyachi at Okayama University was the first user in the world to install an Isolera™ Dalton in his lab, which allowed fractionation and collection of optical isomers. ...
11 March 2015Chugai Pharmaceutical in Japan have worked through nearly every piece of flash equipment from Biotage. Here they explain why - and what they expect from the future. Three research ch...
09 December 2014Ever wondered about the do's and don'ts on the Isolera™ Dalton flash chromatography system with mass detection? This guide summarizes what you need to know in order to reduce down-time and ke...
04 November 2014A new study on ion suppression and mass directed flash chromatography shows the robustness of Isolera™ Dalton. This paper looks at the impact of different solvents on ionization and ...
16 October 2014Biotage will attend the 66th Southeastern Regional Meeting of the American Chemical Society October 16th - 19th, 2014Nashville, TN Come and visit our booth and check out...
13 October 2014Fragrance compounds, being invisible to UV, can be elusive and difficult to isolate. In this application, a four component mixture of fragrance compounds was purified using Accelerated Chromatograp...
23 September 2014Biotage is attending MipTec 2014, Europe’s leading event for Drug Discovery and Life Sciences Research. “Translating Science into Drugs” 23-25 September 2014Basel, Switzerl...
08 August 2014Deletion sequences in peptide chemistry can be difficult to separate because they are very similar to intended products. HPLC has previously been assumed to be the only effective method for these s...
07 August 2014Flash Purification Has Come of Age Flash purification is a chromatographic technique developed with one goal in mind – to deliver the maximum amount of purified product in the minimum ...
06 August 2014Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
27 June 2014Uppsala, Sweden, 27th June, 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of ACI&trad...
01 April 2014Biotage is attending Analytica1-4 April 2014, Munich, Germany Introducing the latest addition to the Biotage family of sample preparation instruments! It's all happening in Hall A1 on Booth 221&n...
16 March 2014Biotage is attending ACS Spring 2014 in Dallas "Chemistry & Materials for Energy" Don't miss Wendy Hartsock and Bob Bickler's talk on "Mass directed flash of peptides". https://www.acs....
14 March 2014By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
10 March 2014Today, flash chromatography instruments are evolving with the built-in capability of determining masses of individual components in the crude mixture, and allowing separation by molecular mass. The...
21 January 2014Biotage has released a new application note for Isolera™ Dalton mass directed flash purification. Synopsis: We demonstrate the mass-directed purification of a simple peptide usin...
27 November 2013Peptide Synthesis, Purification, Mass Identification and Evaporation using the full range of Biotage instruments and consumables. Biotage® Initiator+ Alstra™ is a fully automated microwa...
11 November 2013Labstract recently took delivery of the first Biotage Isolera™ Dalton flash chromatography system in the UK. In an exclusive arrangement with Biotage, Labstract will host & use the equipm...
17 July 2013The webinar titeled "Isolera™ Dalton, the world’s first truly integrated Mass Directed automated Flash Purification System" held today by Dr. Sunil Rana is available online.
17 July 2013Isolera™ Dalton, the world’s first truly integrated Mass Directed automated Flash Purification System Wednesday 17th July 2013 11:00am (CET) / 10:00am (BST) Presented by: Sunil R...
30 May 2013Uppsala, Sweden, 30th May, 2013 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of the new I...
04 May 2013Isolera™ Dalton integrates the Biotage approach to flash chromatography with true compound identification using mass detection. For the first time, Flash chemists can automatically collect t...
04 September 2012Uppsala, Sweden, 4th September, 2012 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces that Biotage is in late s...
The Isolera™ Dalton system consists of Isolera™ Dalton Mass Detector, Isolera™ Dalton Nanolink and Isolera™ Spektra and embraces ACI™ for efficient and automated purification. Isolera Dalton directs flash fractionation by molecular mass, and in real time.
Isolera™ Dalton brings mass detection to flash chromatography. This system identifies compounds by mass in real time during Flash separation, leading to greater confidence in purification and a significant saving in time and money. This combination of identification and purification removes complex off-line analytical steps from the workflow, vastly increasing throughput and putting the entire purification and analysis in the hands of the chemist.
Reactor content can be injected directly to identify target masses, which then are transferred directly to the Flash method for mass targeted purification.
Isolera™ Dalton automates and takes the hard work out of the compound identification and purification, perfect for chemists whose priorities lie more towards the chemistry than small details of running the analysis. Minimal user intervention required makes it available to chemists unfamiliar with mass detection or those whose priorities lie in compound synthesis rather than analysis.
Isolera™ Dalton Mass Detector uses a chip-based electrospray system with a miniaturized quadrupole and internal vacuum pumps, allowing the system to easily fit in a fume hood, and is capable of handling both normal and reverse-phase samples with ease.
Seamless interaction between a flash system and a mass detector requires advanced technology and fine tuning. This is all taken care of by the Isolera™ Dalton Nanolink interface, an intelligent sampling device that at any given moment provides Isolera Dalton Mass Detector with the exact amount of sample required, and dynamically adjusts to the solvent effluent flow rate employed in the Flash system.
Isolera™ Spektra software with its graphic user interface provides a straightforward controlling environment, giving easy access to the process.
Part No. | Description | Product type | Pack Size | Price | ||
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ISO-SPK | Isolera Spektra Package | Accessories | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
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413711 | Mass Detector spray-chip 200 (1pcs) | Spare parts | 1 | Log in for price | |
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413712 | Mass Detector Vac-chip 100 (1pcs) | Spare parts | 1 | Log in for price |