ISOLUTE® C18

SPE Columns and Plates

Biotage sample preparation column size table

ISOLUTE® C18 is a retentive, non-polar sorbent for extraction of a range of analytes from aqueous samples using a non-polar retention mechanism.

Chemical description	Octadecyl (non-endcapped) functionalised silica. manufactured using trifunctional silane.
Sorbent type	Non-polar
Average particle size	50 µm
Pore diameter	60 Å

» MANUALS & USER GUIDES

QuickStart Guide to SPE
Tags: English, Manuals & User Guides

Part No: UI331 Published: 2013 2.35 MB Format: pdf Download | Request copy

Sample Preparation Techniques for the Isolation of Drugs

•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Tags: Manuals & User Guides

Part No: Published: 2007 0.48 MB Format: pdf Download

Step By Step Guide To SPE Method Development

The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides

Part No: Published: 2002 0.36 MB Format: pdf Download

» MATERIAL SAFETY DATA SHEETS

» POSTERS

Method modifications to FDA method LIB4306 allow for the determination of chloramphenicol residues in food grade enzyme powders by SPE-LC-ESI-MS/MS

The fermentation process associated with the manufacture of food grade enzyme powders was recently determined to support the colonization of bacteria. To control this issue, manufactures following different international regulatory procedures have found the addition of familiar antibiotic compounds to manufacturing processes beneficial. This becomes an issue of public health as the concentration of antibiotic residues in food grade materials promotes unknown exposure to the global community. Continued interest in the biomonitoring of these compounds have inspired a number of method development strategies; however, classic methods are labor intensive and require multi-step time consuming efforts. The FDA has recently mandated a method for the determination of chloramphenicol in shellfish. This study evaluates the feasibility of method modifications to incorporate enzyme powder matrices into this method. Linearity was determined over a range of 0.1 – 5 ng/mL (r^2 > 0.990). Relative recovery was determined >80%. The typical repeatability (%RSD) for n=7 replicates <20%. Strategies to control solids prior to SPE load will be presented as they were determined critical in method performance. Method robustness was determined by evaluating multiple lots and multiple variants of enzyme powder origin. The key variable is the nature of the excipients as high level sugars of different sources need to be controlled to minimize the ESI suppression.
Tags: Antibiotics, Chloramphenicol, Column, Enzyme powder, LC-MS/MS, Posters

Part No: P092 Published: 2014 0.36 MB Format: pdf Download

An automated dual stage solid phase extraction procedure for 15 F2t-isoprostane (8-iso Prostaglandin2a) from BSA as lipid markers of oxidative stress

This poster describes an automated procedure for isolation and clean up of 15 F2t-isoprostane (8-iso Prostaglandin 2a)(a lipid marker of oxidative stress) from BSA.
Tags: 15 F2t-isoprostane, 8-iso Prostaglandin2a, BSA, Biological fluids, Biomarker, Clinical, Column, English, GC-MS, Lipids, Posters, RapidTrace

Part No: P059 Published: 2013 0.52 MB Format: pdf Download

» PRODUCT NOTES

Search all documents »

News

13 August 2018

New Findings presented at ASMS 2018

Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...

11 January 2018

The 2018 Analytical Catalog is Here

The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...

17 July 2017

Biotage Goes Direct in Italy

On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...

28 June 2017

Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis

This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...

15 June 2017

Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...

20 March 2017

Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil

This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...

20 February 2017

Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+

This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...

05 January 2016

2016 Analytical Sample Preparation Catalog Released

Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...

11 August 2015

Why Dilute and Shoot Ruins Your Results

Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...

27 March 2015

New Video – Thirty Years of Packing Flash Columns

Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...

08 January 2015

2015 Analytical Sample Preparation Catalog Released

Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...

29 July 2014

Free Shipping on EU Web Orders

Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...

Q&A

ISOLUTE C18 vs. C18 (EC) vs. MFC 18

01 July 2013

Question:

What is the difference between ISOLUTE C18, C18 (EC) and MFC 18?

Answer:

Bonded silicas are formed by the reaction of activated silica with various organosilanes.

ISOLUTE C18 (Sorbent code 220)

A non-endcapped C18 manufactured using a trifunctional octadecyl silane.

This non-endcapped C18 sorbent is designed to exhibit enhanced secondary silanol interactions which can be very useful for example in the extraction of basic drugs. A modifier (up to 5% (v/v) formic acid or ammonium hydroxide) may be needed in the elution solvent to elute basic analytes. Non-endcapped C18 has a lower carbon loading than the endcapped sorbent.

Mean particle size: 50 um
Pore size: 60 A
Endcapped?: No
Typical carbon load: 17.5%

ISOLUTE C18(EC) (Sorbent code 221)

An endcapped C18 manufactured using a trifunctional octadecyl silane.

ISOLUTE C18 (EC) sorbent is manufactured using a trifunctional silane chemistry, and the residual silanols on the silica surface are subsequently endcapped to minimise secondary silanol interactions.

Mean particle size: 50 um
Pore size: 60A
Endcapped?: Yes
Typical carbon load: 18%

ISOLUTE MFC18 (Sorbent code 240)

A non-endcapped monofunctional C18 manufactured using a mono-functional octadecyl silane).

ISOLUTE MFC18 is similar to the classic C18 Sep-Pak (Waters) chemistry, and like the non-endcapped trifunctional ISOLUTE C18, provides useful secondary silanol interactions. The accessibility of these silanol groups to analytes and solvents is increased in MF C18, compared with the trifunctional C18 sorbents. A modifier (up to 5% (v/v) formic acid or ammonium hydroxide) may be needed in the elution solvent to elute basic analytes.

Nominal particle size: 50um
Pore size: 125 A
Endcapped?: No
Typical carbon load: 13 %

Browse more questions and answers >

SPE column capacity

01 July 2013

Question:

What is the maximum capacity of an SPE column?

Answer:

The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.

For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.

Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.

Browse more questions and answers >

Structure of ISOLUTE C18

ISOLUTE® C18 is the most commonly used C18 sorbent for the extraction of acidic, neutral and basic compounds from aqueous sample matrices. Secondary silanol interactions can be exploited to enhance extract purity and method robustness for basic analytes.

ISOLUTE C18 is available in a wide range of SPE columns and 96-well plate formats.

ISOLUTE C18 is also available as loose sorbent (100g pack).

CONSUMABLES

Part No.	Description	Product type	Pack Size	Price
220-0002-A	ISOLUTE® C18 25 mg/1 mL	Consumables	100		Log in for price
220-0005-A	ISOLUTE® C18 50 mg/1 mL	Consumables	100		Log in for price
220-0010-A	ISOLUTE® C18 100 mg/1 mL	Consumables	100		Log in for price
220-0010-B	ISOLUTE® C18 100 mg/3 mL	Consumables	50		Log in for price
220-0010-BG	ISOLUTE® C18 100 mg/3 mL (Tabless)	Consumables	50		Log in for price
220-0010-G	ISOLUTE® C18 100 mg/10 mL (1 mL XL)	Consumables	50		Log in for price
220-0020-B	ISOLUTE® C18 200 mg/3 mL	Consumables	50		Log in for price
220-0020-C	ISOLUTE® C18 200 mg/6 mL	Consumables	30		Log in for price
220-0020-H	ISOLUTE® C18 200 mg/10 mL (3 mL XL)	Consumables	50		Log in for price
220-0025-P01	ISOLUTE®-96 C18 25 mg Fixed Well Plate	Consumables	1		Log in for price
220-0050-B	ISOLUTE® C18 500 mg/3 mL	Consumables	50		Log in for price
220-0050-BG	ISOLUTE® C18 500 mg/3 mL (Tabless)	Consumables	50		Log in for price
220-0050-C	ISOLUTE® C18 500 mg/6 mL	Consumables	30		Log in for price
220-0050-CG	ISOLUTE® C18 500 mg/6 mL (Tabless)	Consumables	30		Log in for price
220-0050-H	ISOLUTE® C18 500 mg/10 mL (3 mL XL)	Consumables	50		Log in for price
220-0050-L	ISOLUTE® C18 500 mg/6 mL (Glass)	Consumables	30		Log in for price
220-0050-P01	ISOLUTE®-96 C18 50 mg Fixed Well Plate	Consumables	1		Log in for price
220-0100-B	ISOLUTE® C18 1 g/3 mL	Consumables	50		Log in for price
220-0100-C	ISOLUTE® C18 1 g/6 mL	Consumables	30		Log in for price
220-0100-CG	ISOLUTE® C18 1 g/6 mL (Tabless)	Consumables	30		Log in for price
220-0100-P01	ISOLUTE®-96 C18 100 mg Fixed Well Plate	Consumables	1		Log in for price
220-0200-C	ISOLUTE® C18 2 g/6 mL	Consumables	30		Log in for price
220-0200-D	ISOLUTE® C18 2 g/15 mL	Consumables	20		Log in for price
220-0500-E	ISOLUTE® C18 5 g/25 mL	Consumables	20		Log in for price
220-1000-F	ISOLUTE® C18 10 g/70 mL	Consumables	16		Log in for price
220-2000-F	ISOLUTE® C18 20 g/70 mL	Consumables	16		Log in for price
9220-0100	ISOLUTE® C18 Bulk 100 g	Consumables	1		Log in for price