
ISOLUTE® C2 is a weakly non-polar sorbent for extraction of non-polar acidic, neutral and basic analytes from aqueous samples using a non-polar retention mechanism.
Chemical description | Ethyl (non-endcapped) |
Sorbent type | Non-polar |
Average particle size | 50 µm |
Pore diameter | 60 Å |
The following method was developed for the extraction of basic drugs from urine or serum using a non-polar retention mechanism.
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Basic,
Clinical,
Serum,
Urine
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing
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QuickStart Guide to SPE
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Manuals & User Guides
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
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The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
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Laboratory chemical
Not for therapeutic or diagnostic use
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Material Safety Data Sheets
This technical note describes the extraction of basic drugs from biological fluids using non-polar ISOLUTE SPE columns.
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The ISOLUTE® family of both endcapped and non-endcapped, non-polar, silica based sorbents are used to extract organic compounds from aqueous matrixes.
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Non-polar SPE is commonly used for extraction of acidic, neutral and basic drugs from biological fluids. It is particularly useful when a parent drug and metabolites of different functionalities are to be extracted simultaneously, and will provide cleaner extracts than alternative sample preparation techniques such as protein precipitation.
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ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
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28 June 2017This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
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Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >ISOLUTE® C2 is used for the extraction of non-polar acidic, neutral and basic compounds from aqueous sample matrices. Secondary silanol interactions can be exploited to enhance extract purity and method robustness for basic analytes.
ISOLUTE C2 is available in a wide range of SPE columns and 96-well plate formats.
ISOLUTE C2 is also available as loose sorbent (100g pack).
Part No. | Description | Product type | Pack Size | Price | ||
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320-0005-A | ISOLUTE® C2 50 mg/1 mL | Consumables | 100 | Log in for price | |
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320-0010-A | ISOLUTE® C2 100 mg/1 mL | Consumables | 100 | Log in for price | |
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320-0010-B | ISOLUTE® C2 100 mg/3 mL | Consumables | 50 | Log in for price | |
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320-0010-G | ISOLUTE® C2 100 mg/10 mL (1 mL XL) | Consumables | 50 | Log in for price | |
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320-0025-P01 | ISOLUTE®-96 C2 25 mg Fixed Well Plate | Consumables | 1 | Log in for price | |
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320-0050-B | ISOLUTE® C2 500 mg/3 mL | Consumables | 50 | Log in for price | |
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320-0100-P01 | ISOLUTE®-96 C2 100 mg Fixed Well Plate | Consumables | 1 | Log in for price | |
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321-0002-A | ISOLUTE® C2(EC) 25 mg/1 mL | Consumables | 100 | Log in for price | |
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9320-0100 | ISOLUTE® C2 Bulk 100 g | Consumables | 1 | Log in for price |