ISOLUTE® C8

SPE Columns and Plates

Biotage sample preparation column size table

ISOLUTE® C8 is a medium retentive, non-polar sorbent for extraction of a range of analytes from aqueous samples using a non-polar retention mechanism.

Chemical description	Octyl (non-endcapped)
Sorbent type	Non-polar
Average particle size	50 µm
Pore diameter	60 Å

News

11 January 2018

The 2018 Analytical Catalog is Here

The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...

17 July 2017

Biotage Goes Direct in Italy

On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...

28 June 2017

Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis

This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...

15 June 2017

Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...

20 March 2017

Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil

This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...

20 February 2017

Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+

This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...

05 January 2016

2016 Analytical Sample Preparation Catalog Released

Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...

11 August 2015

Why Dilute and Shoot Ruins Your Results

Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...

27 March 2015

New Video – Thirty Years of Packing Flash Columns

Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...

08 January 2015

2015 Analytical Sample Preparation Catalog Released

Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...

29 July 2014

Free Shipping on EU Web Orders

Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...

Q&A

ISOLUTE 96 vs. Array Wells

25 February 2014

Question:

What is the difference between the products Isolute-96 C8 50mg (P/N 290-0050-P01), and Isolute array C8 50mg/1mL wells (P/N 290-0050-R)?

Answer:

ISOLUTE-96 C8 50 mg plate (290-0050-P01) is a fixed well plate with 96 sample positions – QTY 1.

ISOLUTE fixed plate

ISOLUTE Array C8 50 mg/1 mL (290-0050-R) is loose array columns which fit into a well plate style holder, primarily used where the full transition to fixed well plate is not yet completed. QTY: 100 loose array columns. This product requires the base plate 120-1000-P01.

ISOLUTE array column setup

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SPE column capacity

01 July 2013

Question:

What is the maximum capacity of an SPE column?

Answer:

The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.

For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.

Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.

Browse more questions and answers >

CONSUMABLES

Part No.	Description	Product type	Pack Size	Price
290-0002-A	ISOLUTE® C8 25 mg/1 mL	Consumables	100		Log in for price
290-0005-A	ISOLUTE® C8 50 mg/1 mL	Consumables	100		Log in for price
290-0010-A	ISOLUTE® C8 100 mg/1 mL	Consumables	100		Log in for price
290-0010-B	ISOLUTE® C8 100 mg/3 mL	Consumables	50		Log in for price
290-0010-BA	ISOLUTE® C8 100 mg/3 mL (ASPEC Adaptor)	Consumables	50		Log in for price
290-0010-C	ISOLUTE® C8 100 mg/6 mL	Consumables	30		Log in for price
290-0020-B	ISOLUTE® C8 200 mg/3 mL	Consumables	50		Log in for price
290-0020-C	ISOLUTE® C8 200 mg/6 mL	Consumables	30		Log in for price
290-0020-H	ISOLUTE® C8 200 mg/10 mL (3 mL XL)	Consumables	50		Log in for price
290-0025-P01	ISOLUTE®-96 C8 25 mg Fixed Well Plate	Consumables	1		Log in for price
290-0050-B	ISOLUTE® C8 500 mg/3 mL	Consumables	50		Log in for price
290-0050-P01	ISOLUTE®-96 C8 50 mg Fixed Well Plate	Consumables	1		Log in for price
290-0100-B	ISOLUTE® C8 1 g/3 mL	Consumables	50		Log in for price
290-0100-C	ISOLUTE® C8 1 g/6 mL	Consumables	30		Log in for price
290-0100-P01	ISOLUTE®-96 C8 100 mg Fixed Well Plate	Consumables	1		Log in for price
290-0200-CG	ISOLUTE® C8 2 g/6 mL, (Tabless)	Consumables	30		Log in for price
9290-0100	ISOLUTE® C8 Bulk 100 g	Consumables	1		Log in for price

Shopping Cart

ISOLUTE® C8 is the most commonly used C8 sorbent for the extraction of acidic, neutral and basic compounds from aqueous sample matrices. Secondary silanol interactions can be exploited to enhance extract purity and method robustness for basic analytes.

ISOLUTE C8 is available in a wide range of SPE columns and 96-well plate formats.

ISOLUTE C8 is also available as loose sorbent (100g pack).

ISOLUTE® C8

SPE Columns and Plates

» APPLICATION NOTES

» CATALOGS

» MANUALS & USER GUIDES

» MATERIAL SAFETY DATA SHEETS

» POSTERS

» PRODUCT NOTES

Search all documents »

News

The 2018 Analytical Catalog is Here

Biotage Goes Direct in Italy

Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis

Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil

Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+

2016 Analytical Sample Preparation Catalog Released

Why Dilute and Shoot Ruins Your Results

New Video – Thirty Years of Packing Flash Columns

2015 Analytical Sample Preparation Catalog Released

Free Shipping on EU Web Orders

Q&A

ISOLUTE 96 vs. Array Wells

SPE column capacity

CONSUMABLES

ISOLUTE® C8

SPE Columns and Plates

» APPLICATION NOTES

SPE Application Note for EPA Method 625, 525.2 and 8080 Analytes

SPE Method Development Recommendations for Extraction of Steroids from Biological Fluids

SPE Method Development Recommendations for Extraction of Sudan I, II, III and IV Dyes from Foodstuffs

SPE Method Development Recommendations for Extraction of Organochlorine Pesticides from Fruit and Vegetables

SPE Method Development Recommendations for Non-Polar Extraction of Basic Analytes for GC Analysis

SPE Application Note for Barbiturates from Urine using C8 on RapidTrace

SPE Application Note for Barbiturates from Urine (C8 Method)

» CATALOGS

Analytical Sample Preparation 2018 Catalog

» MANUALS & USER GUIDES

QuickStart Guide to SPE

Step By Step Guide To SPE Method Development

» MATERIAL SAFETY DATA SHEETS

Safety Data Sheet ISOLUTE C8

» POSTERS

A new Supported Liquid Extraction (SLE) strategy with liquid chromatography mass spectrometry detection for the determination of JWH018, JWH073, CP47,497, CP47,497-C8, JWH200, JWH019, HU210 and JWH250 in blood

» PRODUCT NOTES

Method Development Guidelines: Solid Phase Extraction Using Non-Polar, Silica Based ISOLUTE® SPE Sorbents for the Extraction of Aqueous Samples

ISOLUTE® Non-Polar SPE Sorbents for Extraction of Drugs from Biological Fluids

GENERAL APPROACH TO THE EXTRACTION OF BASIC DRUGS FROM BIOLOGICAL FLUIDS USING NON-POLAR ISOLUTE SPE COLUMNS

Sample Preparation by Non-Polar SPE Using ISOLUTE® SPE Sorbents

Search all documents »

News

The 2018 Analytical Catalog is Here

Biotage Goes Direct in Italy

Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis

Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis

Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil

Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+

2016 Analytical Sample Preparation Catalog Released

Why Dilute and Shoot Ruins Your Results

New Video – Thirty Years of Packing Flash Columns

2015 Analytical Sample Preparation Catalog Released

Free Shipping on EU Web Orders

Q&A

ISOLUTE 96 vs. Array Wells

SPE column capacity

CONSUMABLES