Sample Preparation
Horizon Food & Environmental Products
Organic Synthesis
Peptide Chemistry
Flash Purification
Work-up Products
Evaporation & Concentration
Scale-Up Products
Biomolecules
Service & Support
- Automated Sample Preparation Instruments
- Manual Sample Preparation & Accessories
- ISOLUTE® FILTER+ High Performance Filtration Plates
- ISOLUTE® PPT+ Protein Precipitation Plates
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
- ISOLUTE® QuEChERS
- ISOLUTE® SLE+ Supported Liquid Extraction Products
- ISOLUTE® SPE Columns and Plates
-
- ISOLUTE® 101
- ISOLUTE® AL-A, AL-N & AL-B
- ISOLUTE® Accessories
- ISOLUTE® C18
- ISOLUTE® C18(EC)
- ISOLUTE® C2
- ISOLUTE® C2(EC)
- ISOLUTE® C4
- ISOLUTE® C8
- ISOLUTE® C8(EC)
- ISOLUTE® CBA
- ISOLUTE® CN
- ISOLUTE® DIOL
- ISOLUTE® ENV+
- ISOLUTE® ENV+ On-line SPE Cartridges
- ISOLUTE® EPH
- ISOLUTE® FL
- ISOLUTE® HAX
- ISOLUTE® HCX
- ISOLUTE® HCX-3
- ISOLUTE® HCX-5
- ISOLUTE® HCX-Q
- ISOLUTE® HM-N Columns
- ISOLUTE® Layered SPE Columns
- ISOLUTE® MFC18
- ISOLUTE® Multimode
- ISOLUTE® Myco
- ISOLUTE® NH2
- ISOLUTE® Na2SO4/FL
- ISOLUTE® O&G
- ISOLUTE® PAH
- ISOLUTE® PE-AX
- ISOLUTE® PH
- ISOLUTE® PSA
- ISOLUTE® SAX
- ISOLUTE® SAX/PSA
- ISOLUTE® SCX
- ISOLUTE® SCX-2
- ISOLUTE® SCX-3
- ISOLUTE® SI
- ISOLUTE® Sodium Sulfate Drying Cartridge
- ISOLUTE® TPH
- EVOLUTE® SPE Columns and Plates
- Biotage® Mikro SPE Products
- Biotage® Selekt Systems
- Isolera™ Systems
- Flash Columns
-
- Biotage® Sfär - Overview
- Biotage® Sfär Silica HC D
- Biotage® Sfär Silica D
- Biotage® Sfär Silica HC
- Biotage® Sfär Silica
- Biotage® Sfär KP-Amino D
- Biotage® Sfär C18 Duo 100 Å 30 μm
- Biotage® Sfär - Samplet®
- Biotage® SNAP XL KP-Sil
- Biotage® SNAP XL KP-NH
- Biotage® SNAP XL Ultra C18
- Biotage® SNAP XL KP-C18-HS
- Flash 75/150/400 Cartridges
- TLC Plates
- Flash Accessories
- Flash 75/150/400 Systems
- Service Agreements - Europe
- Service Agreements - North and Latin America
- Discontinued Products
-
- Biotage® Horizon SmartPrep
- Biotage® SNAP Bio
- XcelVap®
- Biotage® SNAP Ultra
- Biotage® SNAP - Samplet®
- Isolera™ Dalton
- AFFINILUTE™ MIP Columns
- Biotage ZIP®
- Biotage ZIP® Sphere
- Biotage® Advancer
- Biotage® Atlantis
- Biotage® ELSD-1080
- Biotage® Endeavor™
- Biotage® Horizon 1000XL
- Biotage® Horizon 3000XL
- Biotage® Horizon 4790
- Biotage® Horizon SPE-Z
- Biotage® Initiator Classic
- Biotage® Resolux™
- Biotage® SP
- Biotage® Syro Wave™
- Biotage® V-10 Evaporator
- EVOLUTE® ABN
- EVOLUTE® AX
- EVOLUTE® CX
- EVOLUTE® WAX
- EVOLUTE® WCX
- EcoLine Environmental Filters
- FlashMaster Personal Plus
- KILOPREP Systems and Consumables
- RapidTrace®+
- SAM
- SAP
- Speed-Vap® III
- TurboVap® 96
- TurboVap® Classic 500
- TurboVap® Classic II
- TurboVap® Classic LV
ISOLUTE® C8
SPE Columns and Plates
ISOLUTE® C8 is a medium retentive, non-polar sorbent for extraction of a range of analytes from aqueous samples using a non-polar retention mechanism.
| Chemical description | Octyl (non-endcapped) |
| Sorbent type | Non-polar |
| Average particle size | 50 µm |
| Pore diameter | 60 Å |
» APPLICATION NOTES
SPE Application Note for EPA Method 625, 525.2 and 8080 Analytes
This method is suitable for the extraction of a wide range of analytes, as defined in EPA methods 525.2 and 8080, from water using a non-polar retention mechanism. These include PAHs, triazine herbicides, organochlorine pesticides, phthalates and other extractables.
Tags: Application Notes,
Environmental,
Organochlorine pesticides (OCP),
Pesticides,
Water
SPE Method Development Recommendations for Non-Polar Extraction of Basic Analytes for GC Analysis
The following method was developed for the extraction of basic drugs from urine or serum using a non-polar retention mechanism.
Tags: Application Notes,
Basic,
Clinical,
Serum,
Urine
» CATALOGS
Analytical Sample Preparation Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing
Tags: Catalogs
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet ISOLUTE C8
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
» POSTERS
A new Supported Liquid Extraction (SLE) strategy with liquid chromatography mass spectrometry detection for the determination of JWH018, JWH073, CP47,497, CP47,497-C8, JWH200, JWH019, HU210 and JWH250 in blood
The potential harm caused by synthetic cannabinoids is a significant concern since exposures and reports of human fatalities due to these substances is widespread [5]. Detection of these relatively new type designer drugs in blood is essential in forensic toxicology, other scientific and medical fields.
Cannabinoids, SLE, SLE+, Supported Liquid Extraction,
Tags: CP47,497,
CP47,497-C8,
Column,
Forensic,
HU210,
JWH-018,
JWH-073,
JWH-200,
JWH-250,
JWH019,
LC-MS/MS,
Posters,
Synthetic Cannabinoids (SPICE),
Whole blood
» PRODUCT NOTES
General Approach to the Extraction of Basic Drugs from Biological Fluids Using Non-Polar ISOLUTE® SPE Columns
This technical note describes the extraction of basic drugs from biological fluids using non-polar ISOLUTE SPE columns.
Tags: Product Notes
Method Development Guidelines: Solid Phase Extraction Using Non-Polar, Silica Based ISOLUTE® SPE Sorbents for the Extraction of Aqueous Samples
The ISOLUTE® family of both endcapped and non-endcapped, non-polar, silica based sorbents are used to extract organic compounds from aqueous matrixes.
Tags: Product Notes
ISOLUTE® Non-Polar SPE Sorbents for Extraction of Drugs from Biological Fluids
Non-polar SPE is commonly used for extraction of acidic, neutral and basic drugs from biological fluids. It is particularly useful when a parent drug and metabolites of different functionalities are to be extracted simultaneously, and will provide cleaner extracts than alternative sample preparation techniques such as protein precipitation.
Tags: Product Notes
Sample Preparation by Non-Polar SPE Using ISOLUTE® SPE Sorbents
ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
Tags: Product Notes
Search all documents »
News
13 August 2018New Findings presented at ASMS 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
11 January 2018The 2018 Analytical Catalog is Here
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
ISOLUTE 96 vs. Array Wells
25 February 2014Question:
What is the difference between the products Isolute-96 C8 50mg (P/N 290-0050-P01), and Isolute array C8 50mg/1mL wells (P/N 290-0050-R)?
Answer:
ISOLUTE-96 C8 50 mg plate (290-0050-P01) is a fixed well plate with 96 sample positions – QTY 1.
ISOLUTE Array C8 50 mg/1 mL (290-0050-R) is loose array columns which fit into a well plate style holder, primarily used where the full transition to fixed well plate is not yet completed. QTY: 100 loose array columns. This product requires the base plate 120-1000-P01.
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >
ISOLUTE® C8 is the most commonly used C8 sorbent for the extraction of acidic, neutral and basic compounds from aqueous sample matrices. Secondary silanol interactions can be exploited to enhance extract purity and method robustness for basic analytes.
ISOLUTE C8 is available in a wide range of SPE columns and 96-well plate formats.
ISOLUTE C8 is also available as loose sorbent (100g pack).
