Sample Preparation
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-
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- ISOLUTE® SI
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- AFFINILUTE™ MIP Columns
- Service Agreements - Europe
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- Discontinued Products
-
- Biotage® Syro Wave™
- Biotage® Advancer
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- SAM
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- TurboVap® Classic LV
ISOLUTE® HCX
SPE Columns and Plates
ISOLUTE® HCX is a mixed-mode sorbent providing clean extracts for basic compounds from complex biological fluid samples using a dual retention mechanism.
| Chemical description | Octyl (C8, non-endcapped) and sulfonic acid (SO3-) |
| Sorbent type | Mixed-mode (non-polar and strong cation exchange) |
| Average particle size | 50 µm |
| Pore size | 60 Å |
» APPLICATION NOTES
Extraction of Acidic, Neutral and Basic Drugs from Urine using ISOLUTE® HCX Columns
The method addresses the extraction of all three classes of drugs of abuse from urine using non-polar and cation exchange retention mechanisms on the ISOLUTE HCX mixed mode column. This protocol was optimized to eliminate the use of dichloromethane in the elution of the acidic / neutral drug fraction.
Tags: Acidic drugs,
Amphetamine,
Application Notes,
Basic drugs,
Benzodiazepines,
Benzoylecgonine,
Cocaines,
Codeine,
Column,
Diazepam,
Forensic,
Morphine,
Naphyrone,
Norcocaine,
Nordiazepam,
Opiates,
Oxazepam,
THC-COOH,
Temazepam,
Urine
Extraction of LSD from Whole Blood using ISOLUTE® HCX Columns
This method was developed for the extraction of lysergic acid diethylamide (LSD) from whole blood using a mixed non-polar and cation exchange retention mechanism.
LSD, BIOTAGE, ISOLUTE, HCX, DOA, Hallucinogen
Tags: Application Notes,
Column,
Forensic,
HPLC,
Hydromorphone,
Whole blood
Extraction of Phencyclidine from Urine using ISOLUTE® HCX Columns
This application was developed for the extraction of phencyclidine and other basic drugs from urine for drugs of abuse applications. Typical recovery for PCP is > 85%.
PCP, DOA, Biotage, ISOLUTE, UCT CleanScreen DAU, Varian Bond Elut Certify
Tags: Application Notes,
Column,
Forensic,
GC-MS,
Phencyclidine (PCP),
Urine
Extraction of Amphetamine and Methamphetamine from Urine using ISOLUTE® HCX Columns
This method was developed for the extraction of amphetamine and methamphetaminefrom urine for drugs of abuse applications. Typical absolute recoveries for these drugs are > 80%.
drugs of abuse
Tags: Amphetamine,
Application Notes,
Forensic,
GC-MS,
Methamphetamine,
Urine
Extraction of Barbiturates from Urine using ISOLUTE® HCX Columns
The following method was developed for the extraction of barbiturate drugs from urine, including: butabarbital, amobarbital, cyclobarbital and phenobarbital. Typical recoveries for these drugs are > 85%.
ISOLUTE, BIOTAGE, HCX, DOA, Nervous System
Tags: Amobarbital,
Application Notes,
Barbiturates,
Butabarbital,
Clinical,
Cyclobarbital,
GC-MS,
Phenobarbital,
Urine
Extraction of Phencyclidine from Meconium using ISOLUTE® HCX Columns
This method was developed for the extraction of phencyclidine from meconium for drugs ofabuse applications. The analyte has an ionisable amine group, which is exploited through cation exchange interactions. The pKa is approximately 8, so the compound is fully ionized at pH values of less than 6. The analyte also has significant non-polar characteristics, and both hydrophobic and ion exchange retention mechanisms are used to give a clean extract.
ISOLUTE, PCP, DOA, Varian Bond Elut Certify, UCT CleanScreen DAU
Tags: Application Notes,
Clinical,
Column,
GC-MS,
Meconium,
Phencyclidine (PCP)
SPE Application Note for Cocaine and Benzoylecgonine from Urine
This method has been optimized for the extraction of cocaine and benzoylecgonine from urine for drugs of abuse applications. Typical recoveries of these drugs are > 85%. The procedure may be applied to plasma, serum or whole blood with suitable modifications.
Tags: Application Notes,
Benzoylecgonine,
Butylone,
Cocaines,
Column,
Forensic,
GC-MS,
Plasma,
Urine,
Whole blood
Extraction of Cocaine, Benzoylecgonine and Cocaethylene From Whole Blood and Human Tissue Using ISOLUTE® HCX Columns
This application note describes the extraction of cocaine, benzoylecgonine and cocaethylene from whole blood and human tissue using ISOLUTE HCX columns.
Tags: Application Notes,
Benzoylecgonine,
Butylone,
Cocaines,
Column,
Forensic,
LC-MS/MS,
Post-Mortem tissue,
Whole blood
Method for the extraction of Morphine and Codeine from urine using ISOLUTE® HCX and RapidTrace® Workstation
This application note describes an automated extraction procedure for the opiates, morphine and codeine from urine using ISOLUTE HCX and the RapidTrace Workstation.
Opiates, drug testing, DOA, Ion exchange
Tags: Application Notes,
Codeine,
Column,
Forensic,
Morphine,
Opiates,
RapidTrace,
Urine
Method for the Extraction of Basic Drugs of Abuse from Biological Fluids using ISOLUTE® HCX & RapidTrace® Workstation
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly
abused drugs as recommended by The National Institute on Drug Abuse (NIDA).
drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Tags: Amitriptyline,
Amphetamine,
Application Notes,
Benzoylecgonine,
Biological fluids,
Butylone,
Codeine,
Column,
Desipramine,
Forensic,
Imipramine,
Methamphetamine,
Morphine,
Nortryptiline,
Plasma,
RapidTrace,
Tricyclic Antidepressant,
Urine
Method for Extraction of Tricyclic Antidepressants from Whole Blood Using ISOLUTE® HCX Columns and RapidTrace®
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation.
TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
Tags: Amitriptyline,
Application Notes,
Clinical,
Column,
Desipramine,
Imipramine,
Nortryptiline,
RapidTrace,
Tricyclic Antidepressant,
Whole blood
Method for the Extraction of the Cocaine Metabolite, Benzoylecgonine from Urine using ISOLUTE® HCX and RapidTrace®
This application note describes an automated extraction procedure for Cocaine metabolite, Benzoylecgonine from urine using ISOLUTE HCX and RapidTrace Workstation.
Tags: Application Notes,
Benzoylecgonine,
Cocaines,
Column,
Forensic,
RapidTrace,
Urine
Extraction of an Extensive Panel of Significant Therapeutic and Illicit Drugs of Abuse from Biological Fluids using ISOLUTE® HCX
This procedure is recommended for the extraction of extensive panel of significant therapeutic and illicit drugs of abuse from whole blood and urine using mixed-mode cation exchange SPE columns. ISOLUTE HCX is a silica based mixed-mode sorbent that combines non-polar and strong cation exchange retention mechanisms for extraction of basic drugs from biological fluids.
Ion exchange, Drugs of Abuse, DOA, Clinical,
Tags: 2-Hydroxyethylflurazepam,
4-Hydroxynordiazepam,
7-Aminoclonazepam,
7-Aminoflunitrazepam,
7-Aminonitrazepam,
Alprazolam,
Application Notes,
Benzodiazepines,
Bromazepam,
Chlordiazepoxide,
Clinical,
Clonazepam,
Clozapine,
Demethylflunitrazepam,
Desalkylflurazepam,
Diazepam,
English,
Estazolam,
Flunitrazepam,
Flurazepam,
Forensic,
Hydroxyalprazolam,
Hydroxymidazolam,
Hydroxytriazolam,
LC-MS/MS,
Midazolam,
Nitrazepam,
Nordiazepam,
Oxazepam,
Prazepam,
Temazepam,
Triazolam,
Urine,
Whole blood,
Zolpidem
Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE® Mixed-mode SPE Columns and 96-well SPE Plates
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Tags: Application Notes,
Product Notes
General Approach to the Extraction of Basic Drugs from Biological Fluids using ISOLUTE® Confirm HCX Mixedmode SPE Columns
ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.
Tags: Application Notes,
English
SPE Application Note for Extraction of NIDA-5 (SAMHSA-5) Analytes using RapidTrace
This method details extraction of the NIDA-5 (SAMHSA-) drug of abuse classes using the same SPE column (ISOLUTE HCX 130 mg/1 mL) and single set of reagents (8 in total) on the RapidTrace.
Tags: Application Notes,
RapidTrace
SPE Application Note for Codeine and Morhpine from Urine
The following method adresses the extraction of the basic drugs morphine and codeine from urine using non-polar and strong cation exchange retention mechanisms.
Tags: Analgesics,
Application Notes,
Urine
SPE Application Note for 6-monoacetyl Morphine from Urine using RapidTrace
The following method addresses the extraction of 6-monoacetyl morphine from urine using
non-polar and strong cation exchange retention mechanisms. This compound can confirm
heroine use since it is a heroine metabolite, but not a metabolite of codeine or morphine
Tags: Application Notes,
Codeine,
RapidTrace,
Urine
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags: Catalogs
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Sample Preparation Techniques for the Isolation of Drugs
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Tags: Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet ISOLUTE HCX
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
» POSTERS
Sample Preparation and Protein: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel Electrophoresis
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
HPLC2014
Tags: Biological fluids,
English,
HPLC,
LC-MS/MS,
Plasma,
Posters
» PRODUCT NOTES
ISOLUTE® Mixed-Mode SPE Sorbents for Extraction of Drugs from Biological Fluids
Sample preparation techniques such as protein precipitation, supported liquid extraction or non-polar SPE may not be selective enough to give extracts of sufficient purity for low level analysis. In these cases, the selective mixed-mode approach to the extraction of basic and acidic drugs is a suitable alternative, giving very high purity extracts with minimal levels of co-extracted material.
Tags: Product Notes
Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE® Mixed-mode SPE Columns and 96-well SPE Plates
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Tags: Application Notes,
Product Notes
Sample Preparation By Mixed-Mode SPE Using ISOLUTE® HCX
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
Tags: Product Notes
Search all documents »
News
11 January 2018The 2018 Analytical Catalog is Here
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >CONSUMABLES
ISOLUTE® HCX combines non-polar (C8) and strong cation exchange retention (SO3-) mechanisms to isolate basic analytes from complex aqueous matrices such as urine and plasma.
Analytes are initially retained by a non-polar retention mechanism, with inorganic salts, polar interferences such as phospholipids and large molecular weight peptides and proteins passing directly through the sorbent bed. The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte(s). Non-polar interferences are removed using an organic solvent or organic solvent/water wash. Analytes are then eluted in an extra clean fraction.
ISOLUTE HCX is the most commonly used sorbent for extraction of drugs of abuse from biological fluid samples. A number of application notes describing the extraction of common drugs of abuse classes are available.
ISOLUTE HCX is available in a wide range of SPE columns and 96-well plate formats.
