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ISOLUTE® HCX-3
SPE Columns and Plates
ISOLUTE® HCX-3 is a mixed-mode sorbent providing clean extracts for basic compounds from complex biological fluid samples using a dual retention mechanism.
| Chemical description | Octadecyl (C18, non-endcapped) and sulfonic acid (SO3-) |
| Sorbent type | Mixed-mode (non-polar and strong cation exchange) |
| Average particle size | 50 µm |
| Pore diameter | 60 Å |
» APPLICATION NOTES
Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE® Mixed-mode SPE Columns and 96-well SPE Plates
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Tags: Application Notes,
Product Notes
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags: Catalogs
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet ISOLUTE HCX-3
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
» PRODUCT NOTES
Generic Method for the Extraction of Basic Drugs from Biological Fluids using ISOLUTE® Mixed-mode SPE Columns and 96-well SPE Plates
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Tags: Application Notes,
Product Notes
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News
11 January 2018The 2018 Analytical Catalog is Here
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This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
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This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
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05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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905-0002-A | ISOLUTE® HCX-3 25 mg/1 mL | Consumables | 100 | Log in for price | |
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905-0010-A | ISOLUTE® HCX-3 100 mg/1 mL | Consumables | 100 | Log in for price | |
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905-0025-P01 | ISOLUTE®-96 HCX-3 25 mg Fixed Well Plate | Consumables | 1 | Log in for price | |
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905-0100-P01 | ISOLUTE®-96 HCX-3 100 mg Fixed Well Plate | Consumables | 1 | Log in for price | |
ISOLUTE® HCX combines non-polar (C18) and strong cation exchange retention (SO3-) mechanisms to isolate basic analytes from complex aqueous matrices such as urine and plasma.
Analytes are initially retained by a non-polar retention mechanism, with inorganic salts, polar interferences such as phospholipids and large molecular weight peptides and proteins passing directly through the sorbent bed. The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte(s). Non-polar interferences are removed using an organic solvent or organic solvent/water wash. Analytes are then eluted in an extra clean fraction.
A good alternative to ISOLUTE HCX for basic compounds that requiremore retentive character from the mixed-mode sorbent.
ISOLUTE HCX-3 is available in a wide range of SPE columns and 96-well plate formats.
