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ISOLUTE® HCX-3

SPE Columns and Plates

ISOLUTE HCX3
Biotage sample preparation column size table 905-0010-a 905-0010-a
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ISOLUTE® HCX-3 is a mixed-mode sorbent providing clean extracts for basic compounds from complex biological fluid samples using a dual retention mechanism.

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ISOLUTE® HCX combines non-polar (C18) and strong cation exchange retention  (SO3-) mechanisms to isolate basic analytes from complex aqueous matrices such as urine and plasma.

Analytes are initially retained by a non-polar retention mechanism, with inorganic salts, polar interferences such as phospholipids and large molecular weight peptides and proteins passing directly through the sorbent bed. The pH is then adjusted to allow an ion exchange retention mechanism to retain the analyte(s). Non-polar interferences are removed using an organic solvent or organic solvent/water wash. Analytes are then eluted in an extra clean fraction.

A good alternative to ISOLUTE HCX for basic compounds that requiremore retentive character from the mixed-mode sorbent.

ISOLUTE HCX-3 is available in a wide range of SPE columns and 96-well plate formats.