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ISOLUTE® Layered SPE Columns
For extracting multiple analyte suites
ISOLUTE® Layered SPE columns are designed for efficient extraction of multi-analyte suites. Layers of different SPE sorbents are contained in a single SPE column, enabling a broader range of analytes to be extracted than with a single sorbent.
» APPLICATION NOTES
Optimizing Extraction of Multianalyte Suites from Water Samples Using Layered Solid Phase Extraction Columns
This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.
Tags: 2,4,6-trichlorophenol,
2,4-D,
2,4-dichlorophenol,
2,4-dimethylphenol,
2,4-dinitrophenol,
2,6-dichlorophenol,
2,6-dinitro-2-methylphenol,
2-methylphenol,
2-nitrophenol,
3-methylphenol,
4-chloro-3-methylphenol,
4-methylphenol,
4-nitrophenol,
Acenapthalene,
Acid herbicides,
Ametrine,
Anthracene,
Application Notes,
Arachlor,
Atrazine,
Base-neutral herbicides,
Bentazone,
Benzo(a)anthracene,
Benzo(a)pyrene,
Benzo(b)fluoranthene,
Benzo(ghi)perylene,
Benzo(k)Fluoranthene,
Chrysene,
Column,
Desethyl atrazine,
Desisopropyl atrazine,
Dibenzo(ah)anthracene,
Dibenzylbutyl phthalate,
Dibutyl phthalate,
Diethyl phthalate,
Dimethyl phthalate,
Dioctyl phthalate,
English,
Environmental,
Environmental contaminants,
Fluoranthene,
Fluorene,
GC-MS,
Indeno(123-cd)pyrene,
Indeno(123-cd)pyrene,
LC-MS/MS,
MCPA,
MCPB,
Metribuzin,
Molinate,
Organochlorine pesticides (OCP),
Organophosphate Pesticide,
Organophosphates,
PAHs,
Persistant organic pollutants (POP),
Pesticides,
Phenanthrene,
Phenols,
Phthalates,
Polyaromatic Hydrocarbons (PAH),
Polyaromatic hydrocarbons,
Product Notes,
Pyrene,
Water
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags: Catalogs
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» PRODUCT NOTES
Optimizing Extraction of Multianalyte Suites from Water Samples Using Layered Solid Phase Extraction Columns
This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.
Tags: 2,4,6-trichlorophenol,
2,4-D,
2,4-dichlorophenol,
2,4-dimethylphenol,
2,4-dinitrophenol,
2,6-dichlorophenol,
2,6-dinitro-2-methylphenol,
2-methylphenol,
2-nitrophenol,
3-methylphenol,
4-chloro-3-methylphenol,
4-methylphenol,
4-nitrophenol,
Acenapthalene,
Acid herbicides,
Ametrine,
Anthracene,
Application Notes,
Arachlor,
Atrazine,
Base-neutral herbicides,
Bentazone,
Benzo(a)anthracene,
Benzo(a)pyrene,
Benzo(b)fluoranthene,
Benzo(ghi)perylene,
Benzo(k)Fluoranthene,
Chrysene,
Column,
Desethyl atrazine,
Desisopropyl atrazine,
Dibenzo(ah)anthracene,
Dibenzylbutyl phthalate,
Dibutyl phthalate,
Diethyl phthalate,
Dimethyl phthalate,
Dioctyl phthalate,
English,
Environmental,
Environmental contaminants,
Fluoranthene,
Fluorene,
GC-MS,
Indeno(123-cd)pyrene,
Indeno(123-cd)pyrene,
LC-MS/MS,
MCPA,
MCPB,
Metribuzin,
Molinate,
Organochlorine pesticides (OCP),
Organophosphate Pesticide,
Organophosphates,
PAHs,
Persistant organic pollutants (POP),
Pesticides,
Phenanthrene,
Phenols,
Phthalates,
Polyaromatic Hydrocarbons (PAH),
Polyaromatic hydrocarbons,
Product Notes,
Pyrene,
Water
Search all documents »
News
11 January 2018The 2018 Analytical Catalog is Here
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17 July 2017Biotage Goes Direct in Italy
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28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
06 September 2016Improved Environmental Monitoring with Layered SPE Columns
The innovative layered SPE technique is used in applications where multiple suites of analytes with a broad polarity range, such as organochlorine, triazine and organophosphate pesticides, or endoc...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
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08 January 20152015 Analytical Sample Preparation Catalog Released
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29 July 2014Free Shipping on EU Web Orders
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Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
|
924-0100-C | ISOLUTE® SAX/PSA 1 g/6 mL | Consumables | 30 | Log in for price | |
|
932-0035-B | ISOLUTE® C18(EC)/ENV+ 350 mg/3 mL | Consumables | 50 | Log in for price | |
|
933-0050-B | ISOLUTE® C2/C18(EC) 500 mg/3 mL | Consumables | 50 | Log in for price | |
|
933-0100-C | ISOLUTE® C2/C18(EC) 1 g/6 mL | Consumables | 30 | Log in for price | |
|
934-0040-C | ISOLUTE® C8/ENV+ 400 mg/6 mL | Consumables | 30 | Log in for price | |
|
|
935-0040-C | ISOLUTE® C18/ENV+ 400 mg/6 mL | Consumables | 30 | Log in for price | |
|
973-0170-B | ISOLUTE® ASCIL/SCX-2/SIL 1.7 g/3 mL | Consumables | 50 | Log in for price | |
|
976-0400-C | ISOLUTE® NA2S04/FL 4 g/6 mL | Consumables | 30 | Log in for price | |
|
981-0100-B | ISOLUTE® SCX-2/SAX 1G/3ML | Consumables | 50 | Log in for price | |
| 982-0100-B | ISOLUTE® ACID SIL/SCX 1 g/3 mL | Consumables | 50 | Log in for price | ||
- Extract multiple suites with a single column
- Improve productivity
- Reduce costs
This innovative SPE technique is used in applications whereanalytes with a broad polarity range are to be extracted simultaneously frm a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.
Sample containing multiple analyte suites of very different chemical characteristics can be efficiently extracted using layered columns. This means that a single sample can be collected and extracted in order to determine multiple analyte suites, whereas separate samples would be needed if more than one extraction column was required. The layered column approach can significantly reduce costs and improve productivity.
Depending on the polarity range of analytes to be extracted, columns with different layers are available.
