
ISOLUTE® Multimode is a mixed-mode sorbent frequently used in 'scavenging' mode for isolation of compounds from complex mixtures.
Chemical description | Octadecyl (non-endcapped), sulfonic acid (SO3-) and quaternary amine (-NR3+) |
Pore diameter | 60 Å |
Average particle size | 50 µm |
Sorbent type | Multimode (non-polar, strong cation exchange, strong anion exchange) |
This method describes the clean-up of acrylamide from cooked foodstuffs, using a multimode retention mechanism (hydrophobic, cation and anion exchange) for retention of interfering food components. The analytical technique is LC-MS-MS.
ISOLUTE, MULTIMODE, SPE, FOOD, FOOD SCARE
Tags: Acrylamide,
Application Notes,
Column,
Food,
Food,
Food contaminant,
LC-MS/MS
ISOLUTE Multimode, ISOLUTE ENV+
Tags: Application Notes
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing
Tags: Catalogs
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
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Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >ISOLUTE® Multimode combines non-polar (C18), strong cation exchange (SO3-) and strong anion exchange (-NR3+) retention mechanisms.
It is most often used in 'scavenging' mode to isolate compounds from complex matrices. In this mode, sample is applied to the SPE column, and interference components (acidic, basic and non-polar) are retained on the column, with the analyte of interest (often small, polar and neutral) passing through to be collected in a purified state. This approach is used for clean up of acrylamide from food matrices.
ISOLUTE Multimode is available in a wide range of SPE column formats.
Part No. | Description | Product type | Pack Size | Price | ||
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904-0010-A | ISOLUTE® Multimode 100 mg/1 mL | Consumables | 100 | Log in for price | |
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904-0030-B | ISOLUTE® Multimode 300 mg/3 mL | Consumables | 50 | Log in for price | |
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904-0030-BA | ISOLUTE® Multimode 300 mg/3 mL (ASPEC Adaptor) | Consumables | 50 | Log in for price | |
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904-0030-C | ISOLUTE® Multimode 300 mg/6 mL | Consumables | 30 | Log in for price | |
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904-0050-B | ISOLUTE® Multimode 500 mg/3 mL | Consumables | 50 | Log in for price | |
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904-0100-C | ISOLUTE® Multimode 1 g/6 mL | Consumables | 30 | Log in for price | |
904-0100-P01 | ISOLUTE®-96 Multimode 100 mg Fixed Well Plate | Consumables | 1 | Log in for price |