Sample Preparation
Horizon Food & Environmental Products
Organic Synthesis
Peptide Chemistry
Flash Purification
Work-up Products
Evaporation & Concentration
Scale-Up Products
Biomolecules
Service & Support
- Automated Sample Preparation Instruments
- Manual Sample Preparation & Accessories
- ISOLUTE® FILTER+ High Performance Filtration Plates
- ISOLUTE® PPT+ Protein Precipitation Plates
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
- ISOLUTE® QuEChERS
- ISOLUTE® SLE+ Supported Liquid Extraction Products
- ISOLUTE® SPE Columns and Plates
-
- ISOLUTE® 101
- ISOLUTE® AL-A, AL-N & AL-B
- ISOLUTE® Accessories
- ISOLUTE® C18
- ISOLUTE® C18(EC)
- ISOLUTE® C2
- ISOLUTE® C2(EC)
- ISOLUTE® C4
- ISOLUTE® C8
- ISOLUTE® C8(EC)
- ISOLUTE® CBA
- ISOLUTE® CN
- ISOLUTE® DIOL
- ISOLUTE® ENV+
- ISOLUTE® ENV+ On-line SPE Cartridges
- ISOLUTE® EPH
- ISOLUTE® FL
- ISOLUTE® HAX
- ISOLUTE® HCX
- ISOLUTE® HCX-3
- ISOLUTE® HCX-5
- ISOLUTE® HCX-Q
- ISOLUTE® HM-N Columns
- ISOLUTE® Layered SPE Columns
- ISOLUTE® MFC18
- ISOLUTE® Multimode
- ISOLUTE® Myco
- ISOLUTE® NH2
- ISOLUTE® Na2SO4/FL
- ISOLUTE® O&G
- ISOLUTE® PAH
- ISOLUTE® PE-AX
- ISOLUTE® PH
- ISOLUTE® PSA
- ISOLUTE® SAX
- ISOLUTE® SAX/PSA
- ISOLUTE® SCX
- ISOLUTE® SCX-2
- ISOLUTE® SCX-3
- ISOLUTE® SI
- ISOLUTE® Sodium Sulfate Drying Cartridge
- ISOLUTE® TPH
- EVOLUTE® SPE Columns and Plates
- Biotage® Mikro SPE Products
- Biotage® Selekt Systems
- Isolera™ Systems
- Flash Columns
-
- Biotage® Sfär - Overview
- Biotage® Sfär Silica HC D
- Biotage® Sfär Silica D
- Biotage® Sfär Silica HC
- Biotage® Sfär Silica
- Biotage® Sfär KP-Amino D
- Biotage® Sfär C18 Duo 100 Å 30 μm
- Biotage® Sfär - Samplet®
- Biotage® SNAP XL KP-Sil
- Biotage® SNAP XL KP-NH
- Biotage® SNAP XL Ultra C18
- Biotage® SNAP XL KP-C18-HS
- Flash 75/150/400 Cartridges
- TLC Plates
- Flash Accessories
- Flash 75/150/400 Systems
- Service Agreements - Europe
- Service Agreements - North and Latin America
- Discontinued Products
-
- Biotage® SNAP Bio
- XcelVap®
- Biotage® SNAP Ultra
- Biotage® SNAP - Samplet®
- Isolera™ Dalton
- AFFINILUTE™ MIP Columns
- Biotage ZIP®
- Biotage ZIP® Sphere
- Biotage® Advancer
- Biotage® Atlantis
- Biotage® ELSD-1080
- Biotage® Endeavor™
- Biotage® Horizon 1000XL
- Biotage® Horizon 3000XL
- Biotage® Horizon 4790
- Biotage® Horizon SPE-Z
- Biotage® Initiator Classic
- Biotage® Resolux™
- Biotage® SP
- Biotage® Syro Wave™
- Biotage® V-10 Evaporator
- EVOLUTE® ABN
- EVOLUTE® AX
- EVOLUTE® CX
- EVOLUTE® WAX
- EVOLUTE® WCX
- EcoLine Environmental Filters
- FlashMaster Personal Plus
- KILOPREP Systems and Consumables
- RapidTrace®+
- SAM
- SAP
- Speed-Vap® III
- TurboVap® 96
- TurboVap® Classic 500
- TurboVap® Classic II
- TurboVap® Classic LV
ISOLUTE® NH2
SPE Columns and Plates
ISOLUTE® NH2 is a weak anion exchange sorbent used to extract acids from aqueous samples. Alternatively, it can be used as a polar sorbent to extract polar analytes from non-aqueous matrices.
| Chemical description | Aminopropyl (non-endcapped) functionalized silica. Manufactured using trifunctional silane. |
| Sorbent type | Weak anion exchange. Can also be used as a polar sorbent. |
| Average particle size | 50 µm |
| Pore diameter | 60 Å |
| Exchange capacity | 0.6 meq/g |
| pKa | 9.8 |
» APPLICATION NOTES
A No-Drydown SPE Method for Biomarkers of Alcohol Consumption in Human Urine Using ISOLUTE® NH2 SPE Columns Prior to LC-MS/MS
This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.
Tags: Alcohol metabolite,
Application Notes,
Clinical,
Column,
Ethyl Sulfate (EtS),
Ethyl glucuronide (EtG),
Forensic,
LC-MS/MS,
Urine
SPE Method Development Recommendations for Extraction of Ethyl Glucuronide from Biological Fluids
This is a suggested emthod for the extraction of ethyl glucuronide from biological fluids (urine and serum) using a polar retention mechanism.
Tags: Application Notes
SPE Method Development Recommendations for Extraction of Fermentable Sugars from Wort
Using ISOLUTE NH2 SPE column.
Tags: Application Notes
SPE Method Development Recommendations for Sodium Monofluoroactate from Animal Feed
Recomendations for SPe method development. The proposed steps are based on experience with similar analytes and matarices, but have not been verified in Biotage laboratories.
Tags: Animal feed,
Application Notes
» CATALOGS
Analytical Sample Preparation Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing
Tags: Catalogs
» CUSTOMER CASES
Tracing an Ancient Diet
Dr. Simon Hammann is Postdoctoral Researcher at the
Department of Anthropology and Archaeology, University
of Bristol. By combining chemistry with archeological
research, he is able to look back in time and has produced
the first direct chemical evidence for dietary cereal
processing among Romans.
Tags: Customer Cases
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet ISOLUTE NH2
ISOLUTE NH2
9470
Laboratory chemical
Tags: English,
Material Safety Data Sheets
» POSTERS
LC-MS/MS Analysis of Ethyl Glucuronide and Ethyl Sulfate in Urine: A Comparison of Sample Preparation Techniques
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
SOFT 2014
Tags: Alcohol metabolite,
Column,
English,
Ethyl Sulfate (EtS),
Ethyl glucuronide (EtG),
Forensic,
LC-MS/MS,
Posters,
Urine
» PRODUCT NOTES
Method Development Guidelines Solid Phase Extraction Using ISOLUTE® NH2 SPE Sorbents for the Extraction of Aqueous Samples
The ISOLUTE® family of anion exchange sorbents are used to extract organic anions (acidic compounds capable of exhibiting a negative charge) from both aqueous and non-aqueous matrixes.
Anion exchange SPE can be accomplished by strong (very high pKa) or weak (lower pKa) ion exchangers.
Tags: Product Notes
Method Development Guidelines: Solid Phase Extraction Using Polar, Silica Based ISOLUTE® SPE Sorbents for the Extraction of Non-Aqueous Samples
The ISOLUTE® family of polar sorbents are used to extract polar organic compounds from non-polar matrixes.
There are many functional groups that can exhibit polar interactions, and they have different selectivities.Some of the polar interactions that exist include dipole-dipole,dipole-induced dipole and hydrogen bonding. Polar interactions are particularly useful for the separation of molecules that are very similar in structure (e.g. structural isomers.
Tags: English,
Product Notes
Search all documents »
News
30 October 2018Evidence for Ancient Roman Diets Uncovered with ISOLUTE® NH2
Dr. Simon Hammann is Postdoctoral Researcher at the Department of Anthropology and Archaeology, University of Bristol. By combining chemistry with archeological research, he is able to look back in...
13 August 2018New Findings presented at ASMS 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
11 January 2018The 2018 Analytical Catalog is Here
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
18 September 2014Optimized Sample Preparation Method for Alcohol Biomarkers
ISOLUTE® NH2 solid phase extraction cartridges work well for detecting alcohol biomarkers in human urine, and a recent study reported a successful throughput of 6,000 samples per year. The key ...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >
ISOLUTE® NH2 is an aminopropyl bonded sorbent used for the extraction of strongly acidic compounds (e.g. those containing sulfonic acid groups) or analytes unstable under acidic conditions, from aqueous samples using a weak anion exchange retention mechanism. Analyte elution can be performed by neutralizing charge on the sorbent (high pH). The sorbent is supplied as the free base.
Alternatively, ISOLUTE NH2 can be used for extraction of polar compounds from a non-polar matrix using hydrogen bonding retention mechanism. It is less retentive than ISOLUTE SI.
ISOLUTE NH2 is available in a wide range of SPE columns and 96-well plate formats.
ISOLUTE NH2 is also available as loose sorbent (100g pack).
