This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.

4790 Anthracene Application Notes Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Disk EN16691 Analytes EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Surface water

This application note describes the automated solid phase extraction (SPE) and GC/MS analysis of whole water samples according to EN16691 for PAHs. Method EN 16691 is a recently developed method that uses solid phase extraction to isolate organic compounds from 1 L of water using a divinylbenzene (DVB) solid phase extraction disk.

4790 Anthracene Application Notes Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo(b)fluoranthene Benzo(k)Fluoranthene Disk EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Water

This method demonstrates the extraction of a PAH standard test mix comprising of fluoranthene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, indeno(123-cd)pyrene, dibenzo(ah)anthracene, and benzo(ghi)perylene. MIP, AFFINILUTE, SupelMIP, BIOTAGE, PAH, Polyaromatic Hydrocarbons

Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene Environmental GC-MS Indeno(123-cd)pyrene Olive Oil Polyaromatic Hydrocarbons (PAH)

This application note uses the Biotage® Horizon 4790 automated extraction system with a DVB SPE disk to extract an aqueous sample for PAH compounds analysis. The extract is dried using the Horizon Technology DryDisk®.

1-methylnaphthalene 2-methylnaphthalene 3-methylcholanthrene Acenaphthene Acenaphthylene Acenapthalene Anthracene Application Notes Benzo (a) Anthracene Benzo (a) Pyrene Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene English Environmental Fluoranthene GC-MS Napthalene PAHs Perylene-d12 Phenanthrene Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Water

This technical note describes the use of layered SPE columns in applications where multiple suites of analytes with a broad polarity range are to be extracted simultaneously from a single water sample, such as organochlorine, triazine and organophosphate pesticides, or endocrine disrupters.

2,4,6-trichlorophenol 2,4-D 2,4-dichlorophenol 2,4-dimethylphenol 2,4-dinitrophenol 2,6-dichlorophenol 2,6-dinitro-2-methylphenol 2-methylphenol 2-nitrophenol 3-methylphenol 4-chloro-3-methylphenol 4-methylphenol 4-nitrophenol Acenapthalene Acid herbicides Ametrine Anthracene Application Notes Arachlor Atrazine Base-neutral herbicides Bentazone Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Desethyl atrazine Desisopropyl atrazine Dibenzo(ah)anthracene Dibenzylbutyl phthalate Dibutyl phthalate Diethyl phthalate Dimethyl phthalate Dioctyl phthalate English Environmental Environmental contaminants Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Indeno(123-cd)pyrene LC-MS/MS MCPA MCPB Metribuzin Molinate Organochlorine pesticides (OCP) Organophosphate Pesticide Organophosphates PAHs Persistant organic pollutants (POP) Pesticides Phenanthrene Phenols Phthalates Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Product Notes Pyrene Water

US EPA method 550.1 is a method used by regulatory agencies to determine PAH’s in drinking water by liquid-solid extraction (LSE) and high performance liquid chromatography (HPLC).1 The method outlines the steps needed to perform extractions of PAHs from drinking water sources and finished drinking water; as well as the quality control (QC) program requirements to ensure the method is operated under control. Use of Biotage automated sample preparation products allows users to achieve the precise and consistent data needed to comply with EPA method 550.1 QC specifications as stated in method, section 10.3.2., for Initial Demonstration of Capability (IDC); while at the same time, streamlining laboratory practices to increase lab productivity.

4790 Acenaphthene Acenaphthylene Acenapthalene Anthracene Application Notes Benzo (a) Anthracene Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Chrysene Dibenz (a,h) Anthracene Disk Drinking water EPA 550.1 Analytes English Environmental Fluoranthene Fluorene HPLC Indeno (1,2,3-cd) Pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Pyrene