This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

The objective was to develop a GC-MS assay for the determination of free benzodiazepines using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods. ISOLUTE, SLE, SLE+, Supported Liquid Extraction, Benzodiazepines, Forensic, Drugs, DOA, Drugs of Abuse, SOFT,

4-Hydroxynordiazepam 7-Aminoclonazepam 7-Aminoflunitrazepam 7-Aminonitrazepam Benzodiazepines Bromazepam Column Demethylflunitrazepam Flurazepam Forensic GC-MS Hydroxyalprazolam Hydroxymidazolam Hydroxytriazolam Midazolam Nitrazepam Posters Urine

This poster describes an automated procedure for isolation and clean up of 15 F2t-isoprostane (8-iso Prostaglandin 2a)(a lipid marker of oxidative stress) from BSA.

15 F2t-isoprostane 8-iso Prostaglandin2a BSA Biological fluids Biomarker Clinical Column English GC-MS Lipids Posters RapidTrace

Effective removal of residual water from organic solvents is critical to achieve optimal recoveries for water soluble compounds. Sodium sulfate has been the standard technique for removing residual water from solvent extracts for many years; however, it has several major drawbacks. Water soluble analytes in the residual water phase can become adsorbed in the sodium sulfate, leading to lower recoveries.

Application Notes Disk English Environmental GC-MS Organic Compounds Water

Pesticides, including insecticides, herbicides and fungicides are used extensively to increase agricultural yields. The total world usage of pesticides in 2007 was approximately 5.2 billion pounds with the US consuming approximately 22% of the total.1 The wide use of pesticides yields concern that drinking water sources will become contaminated, exposing the population to hazardous substances that may cause cancer.

Application Notes Disk Drinking water EPA 508.1 Analytes English Environmental GC-MS Pesticides

This application note demonstrates that the Biotage(R) Horizon 4790 using Atlantic C18 disks can effectively extract hormone compounds from 1-L water samples in approx 40 mins.

11-ketotestosterone 17-alpha-estradiol 17-alpha-ethynylestradiol 17-beta-estradiol 4790 Androgens Androsterone Application Notes Diethylstilbestrol Dihydrotestosterone (DHT) Disk Endocrine disrupter English Environmental Epitestosterone Estradiol Estriol Estrogens Estrone GC-MS Hormone Hormones Mestranol Norethindrone Progesterone Steroid Steroid Hormones Steroid metabolite Steroids Surface water Testosterone Water

Pesticides have gone far in increasing food supply, important in a world expecting to have 9.8 billion people by 2050. However, the mechanisms that make pesticides effective in eliminating pests can also cause harm to humans and animals. News stories continue to point to contamination through spills and exposure through the respiratory system.

5000 Application Notes Disk EPA 608.3 Analytes English Environmental GC-MS US EPA Method 608.3 Waste water

A GC-MSMS method of simultaneously measuring legacy/emerging POPs PCBs, OCPs and PBDEs) in serum was developed and applied to this study of exposure of mothers to harmful environmental contaminants.

BHC Clinical DDE DDT English Environmental GC-MS HCB Organochlorine pesticides (OCP) Organochlorines Oxychlordane PBDE PBDEs PCB PCBs Persistant organic pollutants (POP) Posters RapidTrace Serum Trans-nonachlor

Polychlorinated Biphenyls (PCB) are a worldwide contamination problem. They are extremely stable and as such, very long lived in the environment. The end result is that PCBs tend to bioaccumulate throughout the food chain and a great deal of research and regulations have been dedicated to their study.

4790 Application Notes English Environmental GC-MS PCB PCBs US EPA Method 8082 Water

In this application note, a suite of neutral and list 1 pesticides in methylene chloride is evaporated using the Biotage® DryVap. Pesticides containing many different functional groups, including sulfur and phosphorus groups, halides, nitramines, atrazines, alcohol, and carbonyl groups were efficiently concentrated for GCMS analysis. This data indicates that the DryVap System is inert and effectively manages solvent evaporation without generating localized hot spots in the system.

Application Notes English Environmental GC-MS Organochlorine pesticides (OCP) Organophosphate Pesticide Pesticides Water

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns. The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) Application Notes Column English Extrahera Forensic GC-MS Hydrolysed urine THC metabolites THC-COOH

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with ISOLUTE SLE+ columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage Extrahera, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) Application Notes Column English Forensic GC-MS Hydrolysed urine THC metabolites THC-COOH

This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using Biotage® Extrahera™ configured for use with ISOLUTE® SLE+ supported liquid extraction columns. Using the Biotage® Extrahera™, 24 hydrolyzed samples were extracted in under 31 minutes and 24 non-hydrolyzed samples were extracted in under 36 minutes. The limits of quantitation meet or exceed the sensitivity requirements set by SAMHSA and EWDTS for workplace testing applications.

6-acetylmorphine (MAM) 96-well plate Application Notes Codeine Dihydrocodeine (DHC) English Extrahera Forensic GC-MS Hydrocodone Hydrolysed urine Hydromorphone Morphine Opiates Opiates Oxycodone Oxymorphone Urine

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.

4790 Anthracene Application Notes Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Disk EN16691 Analytes EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Surface water

This application note describes the automated solid phase extraction (SPE) and GC/MS analysis of whole water samples according to EN16691 for PAHs. Method EN 16691 is a recently developed method that uses solid phase extraction to isolate organic compounds from 1 L of water using a divinylbenzene (DVB) solid phase extraction disk.

4790 Anthracene Application Notes Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo(b)fluoranthene Benzo(k)Fluoranthene Disk EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Water

This application note demonstrates the performance of automated disk-based solid phase extraction (SPE), using Biotage® Horizon 5000 automated extraction system and Atlantic® DVB SPE disks, for concentration of organochlorine pesticides from whole water, prior to GC/MS analysis, following the requirements of EN16693:2015.

1,2 4 trichlorobenzene 1,2,3 trichlorobenzene 1,3,5 trichlorobenzene 5000 Alachlor Aldrin Application Notes DDD DDE DDT Dieldrin Disk Drinking water EN16693 Water Quality EN16693:2015 Analytes Endosulfan Endrin English Environmental GC-MS Hexachlorobenzene Hexachlorobutadiene Isodrin Organochlorine pesticides (OCP) Pentachlorobenzene Water alpha HCH beta HCH delta HCH gamma HCH

Polycyclic aromatic hydrocarbons (PAHs) can be found in a number of water types, such as drinking water, surface water, ground water and wastewater. There are serval types of extraction techniques that can be implemented to remove them from water for measurement including liquid-liquid extraction (LLE), continuous liquid-liquid extraction (CLLE) and solid phase extraction (SPE). With LLE and CLLE there is little selectivity within the methods for difficult matrices or other compound interferences.

5000 Application Notes Disk Drinking water English Environmental GC-MS Ground water PAHs Polyaromatic Hydrocarbons (PAH) Waste water

This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.

5000 Application Notes Bisphenol A Disk Drinking water Endocrine disrupter English Environmental Environmental contaminants GC-MS Nonyl phenol Water

When analyzing human urine for drugs of abuse, one of the most common tests is for the cocaine metabolite Benzoylecgonine (BZE). A contract laboratory has automated this labor intensive procedure using the RapidTrace.

Application Notes Benzoylecgonine Column Drugs of Abuse Forensic GC-MS RapidTrace Urine