This poster describes a strong cation exchange SPE protocol for extraction of Vitamin B3 (Niacin) and related metabolites from serum. The ISOLUTE SCX-3 96 well plate format demonstrated as a viable option for serum measurements over a relevant concentration range in clinical diagnostics. It is anticipated that strategies presented in this poster would be of broad-based interest to clinical laboratories focused on combining parent/metabolite assays into a single analysis. MSACL 2014

96-well plate B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Posters Serum Vitamin B3 Vitamins

This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.

96-well plate Application Notes B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Serum Vitamin B3 Vitamins

This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated. ASMS 2014

96-well plate Biotin Clinical English LC-MS/MS Posters Serum Vitamin B7 Vitamins

Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.

Alcohol metabolite Application Notes Biomarker Brochures Catecholamines Clinical English Hormone Hormones Pain Management Peptides Product Notes Steroid Steroid Hormones Steroid metabolite Steroids Thyroid Hormone Tobacco Specific Nitrosamines (TSNAs) Vitamin D Vitamins Water Soluble Vitamins

This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The manual extraction protocols were transferred to an SPE automation platform (Biotage® Extrahera)and method performance versus manual processing compared. MSACL EU 2015

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English Extrahera LC-MS/MS Posters Serum Vitamin D Vitamins

This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared. ASMS 2015

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Biomarker Clinical English Extrahera LC-MS/MS Posters Serum Vitamin D Vitamins

This poster presents a simple method for the extraction and subsequent detection of both the traditional hydroxy metabolites and the biologically active dihydroxy metabolites in serum. High analyte recoveries and low ion suppression were demonstrated, allowing limits of quantitation at low pg/mL levels for the di-OH metabolites and < 1 ng/mL levels for the OH metabolites. MSACL 2014

1, 25 Dihydroxyvitamin D 1a, 25-dihydroxy-vitamin D 1α, 25-dihydroxy-vitamin D 25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English LC-MS/MS Posters Serum Vitamin D Vitamins

This poster describes a comparison of 25-hydroxy Vitamin D Extraction using Supported Liquid Extraction and Phospholipid Depletion Plate Technology using Manual and Automated Sample Preparation prior to LC/MS Analysis. It was presented at MSACL 2015, San Diego

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English LC-MS/MS Posters Serum Vitamin D Vitamins

ISOLUTE® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation. This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Included in this application note are the detailed settings for implementing the method on the Biotage Extrahera automation system.

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Application Notes Clinical English Extrahera LC-MS/MS Serum Vitamin D Vitamins

This application note describes the extraction of a panel of fat soluble vitamins (including those representing Vitamins A, D, E & K) from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries approximately or above 90% with RSDs 10% or less for all analytes.

25-OH Vitamin D2 25-OH Vitamin D3 96-well plate Application Notes Beta-carotene (Vitamin A) Clinical English Fat Soluble Vitamins LC-MS/MS Menaquinone-4 (Vitamin K2) Phylloquinone (Vitamin K1) Retinol (Vitamin A) Serum Vitamin A (Retinol) Vitamin A (beta-carotene) Vitamin E (alpha-Tocopherol) Vitamin K1 (Phylloquinone) Vitamin K2 (Menaquinone-4) Vitamins

Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from human serum prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from serum allowing for mass spectral detection of the target analytes at a concentration of 100 ng/mL or less. Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Serum, Clinical, LCMS, Sample Prep

Application Notes Clinical LC-MS/MS Retinol (Vitamin A) Vitamin A (Retinol) Vitamin E (alpha-Tocopherol) Vitamins a-Tocopherol β-Carotene

Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from whole blood prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from whole blood allowing for mass spectral detection of the target analytes at a concentration of 200 ng/mL or less. Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Whole Blood, Clinical, LCMS, Sample Prep

Application Notes LC-MS/MS Retinol (Vitamin A) Vitamin A (Retinol) Vitamin E (alpha-Tocopherol) Vitamins Whole blood a-Tocopherol β-Carotene

The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.

96-well plate Application Notes Biotin Clinical English Extrahera LC-MS/MS Serum Vitamin B7 Vitamins Water Soluble Vitamins

Part of the B complex group of water soluble vitamins, vitamin B7 (Biotin) is involved in various metabolic processes within the body. It is a small polar carboxylic acid which can present challenges in bioanalysis. This poster discusses the impact of optimization of various parts of the method development process to maximize sensitivity allowing low level analysis of vitamin B7 from human serum. MSACL 2017, Palm Springs ASMS 2017, Indianapolis

96-well plate Biotin Clinical English LC-MS/MS Posters Serum Vitamin B7 Vitamins Water Soluble Vitamins

This poster presents a novel method for the simultaneous extraction, derivatization and subsequent detection of both the traditional 25-hydroxy and the biologically active 1α,25-dihydroxy vitamin D metabolites in serum. ASMS2014

1, 25 Dihydroxyvitamin D 1a, 25-dihydroxy-vitamin D 1α, 25-dihydroxy-vitamin D 25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English LC-MS/MS Posters Serum Vitamin D Vitamins

This poster describes the development and optimisation of the dual extraction method for Vitamin D metabolites using ISOLUTE SLE+.

1, 25 Dihydroxyvitamin D 1a, 25-dihydroxy-vitamin D 1α, 25-dihydroxy-vitamin D 25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English LC-MS/MS Posters Serum Vitamin D Vitamins