This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs. Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
Our carefully selected portfolio of industrial scale products have a proven track record of successful applications and use in scale-up projects. We can support the discovery, development and manufacturing of customer pharmaceutical and biotechnology products, from pre-clinical, phase I, II and III to small scale commercial operations.
Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
It is imperative that pharmaceutical processes have the flexibility and robustness to quickly output targets of interest – from small molecules and APIs for drug products to complex structures or chemical sensors that may be used in kits or other diagnostic devices. In chemical manufacturing, for example in the pharmaceutical industry, target molecules are synthesized on a large scale in dedicated manufacturing plants. Such processes represent the culmination of a great deal of research and development, where much time and consideration has been spent scaling laboratory based synthetic routes into commercially and practically viable manufacturing procedures. This paper explains the principles of scale up purification.