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AN924_925 Cows

Two Alternative Approaches to the Extraction of a Suite of Regulated Veterinary Growth Promoters

20 November 2019

These two application notes describe alternative approaches to the extraction of a suite of regulated veterinary growth promoters (including steroids, stilbenes and resorcylic acid lactones) from animal urine (cattle, pig, sheep and horse).


Application Note Number AN924

ISOLUTE® SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid liquid extraction (LLE)  for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced preparation time. This application note provides an effective and efficient ISOLUTE SLE+ procedure optimized for the 1 mL  sample capacity column format. 

Download Application Note  AN924



Application Note Number AN925

ISOLUTE® HYDRO DME+ dual mode extraction columns and plates provide extremely efficient removal of matrix components and  hydrolysis enzymes from urine samples, using a simple flow  through workflow. This application note provides an effective and efficient ISOLUTE HYDRO DME+ procedure using column  and 96-well plate formats. 

Download Application Note AN925



  • ß-trenbolone
  • α-trenbolone
  • ß-boldenone
  • α-boldenone
  • ß-nortestosterone
  • α-nortestosterone
  • methylboldenone 
  • methyltestosterone
  • 16-ß-hydroxystanozolo
  • taleranol
  • zeranol
  • ß-zearalenol
  • α zearalenol
  • zearalenone
  • zearalanone
  • diethylstilbestrol,
  • dienestrol
  • hexestrol

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ISOLUTE® SLE+ Supported Liquid Extraction