Recreational marijuana use is currently legal in eleven U.S. states. Fourteen additional states have decriminalized marijuana, but have not legalized it. It is increasingly important for laboratories to develop accurate testing methods for THC, and its related compounds, in biological matrices of those that may be driving or committing crimes under the influence of marijuana. Biotage provides simple and effective sample prep protocols for cleanup of blood, urine, and oral fluid samples that produce reliable results, and are automation compatible.
Tags: 96-well plate, Brochures, Clinical, Column, GC-MS, LC-MS/MS, THC, THC metabolites
This guide describes simple practical strategies, hints and tips designed to mitigate or avoid cross-well contamination or ‘cross talk’ in high throughput 96-well based sample preparation techniques.
Includes a section on controlling evaporative crosstalk in urine
specimens with high drug concentrations.
Tags: 96-well plate, Brochures, Clinical, English, Forensic, Pharma
Biotage® ACT Plate Adapter (patent pending) is designed to reduce or eliminate the occurrence of well-to-well cross contamination (cross talk) during extract evaporation, and fits on top of the 96-well collection plate during evaporation.
Tags: Manuals & User Guides
Hydroxychloroquine (HCQ) and chloroquine (CQ) are common antimalarial drugs with long half-lives. They are currently being tested as potential treatments for COVID-19. Due to their high publicity in the media, many forensic toxicologists are working on developing methods to extract and detect these compounds in different matrices to understand the adverse effects, potential toxicity, and drug interactions associated with these drugs. These compounds are not typically included in general unknown screens. A simple sample preparation procedure that delivers clean extracts and analyte recovery greater than 90% for CQ and greater than 82% for HCQ with minimum matrix effects and high process efficiency was developed.
Tags: Biological fluids, Chloroquine (CQ), Clinical, Hydroxychloroquine (HCQ), LC-MS/MS, Posters
LC-MS/MS analysis of volatile analytes is problematic when 96-well plates are used. Solid phase extraction of urine with high drug concentrations can cause contamination of the elution solvent during evaporation as the compounds volatilize then redistribute in adjacent wells.This phenomenon, known as “evaporative crosstalk”, can generate false positive results.
Tags: Amphetamines, Forensic, LC-MS/MS, Methamphetamine, Posters
Evaporative crosstalk is well-to-well cross contamination
during extract evaporation after SLE or SPE extraction in a 96
well plate. It is often observed with volatile analytes like
methamphetamine and amphetamine, but it can also occur
with other drugs and metabolites when urine samples have
very high analyte concentrations. ASMS, 2020.
Tags: Amphetamine, Benzoylecgonine, Drugs of Abuse, Forensic, Hydromorphone, MDA, MDEA, MDMA, Methamphetamine, Morphine, Posters, Urine
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination.
Tags: 2-Hydroxyethylflurazepam, 2-OH-Et-Flurazepam, 6-acetylmorphine (MAM), 7-Aminoclonazepam, 7-Aminoflunitrazepam, 96-well plate, Alprazolam, Amphetamine, Amphetamines, Benzodiazepines, Bromazepam, Buprenorphine, Clinical, Clonazepam, Codeine, Column, Diazepam, Dihydrocodeine (DHC), Drugs of Abuse, EDDP, English, Estazolam, Extrahera, Fentanyl, Flunitrazepam, Flurazepam, Forensic, GC-MS, Hydrocodone, Hydromorphone, Ketamine, LC-MS/MS, Lorazepam, MDA, MDEA, MDMA, Mephedrone, Methadone, Methamphetamine, Midazolam, Morphine, Nitrazepam, Norbuprenorphine, Nordiazepam, Norfentanyl, Norketamine, Opiates, Opiates, Oxazepam, Oxycodone, Oxymorphone, Phencyclidine (PCP), Posters, Temazepam, Triazolam, Urine, Whole blood, Zaleplone, Zolpidem, Zopiclone, z-drugs, α-OH Triazolam, α-OH alprazolam
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.
Tags: 11-deoxycortisol, 11-keto-androsterone, 11-oxoetiocholanolone, 11-β-hydroxyandrosterone, 11β-hydroxyetiocholanolone, 2-OH-estrone, 2-methoxyestrone, 3α-androstanediol, 3β-androstanediol, 4-OH-estrone, 4-methoxyestrone, 96-well plate, Allo-THB, Androsterone, Clinical, Corticosterone, DHEA (dehydroepiandrosterone, Dehydroepiandrosterone (DHEA), English, Etiocholanolone, Extrahera, Hormone, Hormones, Hydrolysed urine, Posters, Steroid, Steroid Hormones, Steroid metabolite, Steroids, THB, THF, Testosterone, α-THF
This poster discusses various stages of the sample preparation process to determine and assess the potential for cross contamination and present approaches to minimize and or eliminate the effect.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Pharma, Posters
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
Tags: 96-well plate, Clinical, English, Forensic, LC-MS/MS, Opiates, Pharma, Posters
Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing,
SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
Tags: English, Product Notes
This product note details technical and ordering information for the Biotage® ACT Plate Adapter, a novel accessory for prevention of cross talk during evaporation.
Tags: 96-well plate, Clinical, English, Forensic, Pharma, Product Notes
Recovery profiles from manual and automated supported liquid extraction protocols were put side by side in a study on drugs of abuse in blood and urine matrices. Data reliability in forens...16 July 2015
Uppsala, Sweden, 15th July 2015 – Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, announces the launch of Biotage® ...
Designed for high throughput laboratories, the Biotage® SPE Dry 96 and Biotage® SPE Dry 96 Dual Sample Concentrator Systems provide efficient solvent evaporation in microplate format and are compatible with 24-, 96- and 384-well collection plates. Heated gas flow from above and below the collection plate ensures efficient solvent evaporation.