
EVOLUTE® EXPRESS Sample Preparation products provide robust, reliable SPE, giving ultra pure extracts and high analyte recoveries. Developed specifically for analyte quantification by LC-MS/MS, EVOLUTE sorbents provide a highly effective solution to the problems of ion suppression and matrix effects.
Eliminate conditioning and equilibration with EVOLUTE EXPRESS load-wash-elute SPE.
Chemical description | Primary/secondary amine modified polystyrene-divinylbenzene incorporating non-ionizable hydroxyl groups |
Sorbent type | Mixed-mode non-polar/weak anion exchange |
Exchange capacity | 0.3 mmol/g |
Average particle size | 30 µm, 50 µm |
Pore diameter | 40 Å |
pKa | ~10 |
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Tags: Alcohol metabolite,
Application Notes,
Biomarker,
Brochures,
Catecholamines,
Clinical,
English,
Pain Management,
Peptides,
Product Notes,
Steroid Hormones,
Steroid metabolite,
Thyroid Hormone,
Tobacco Specific Nitrosamines (TSNAs),
Vitamin D,
Vitamins,
Water Soluble Vitamins
The method described in this application achieves high reproducible recoveries for a number of common PFCs in water using an optimized weak cation exchange SPE procedure with EVOLUTE® EXPRESS WAX SPE columns.
Tags: Application Notes,
Column,
English,
Environmental,
LC-MS/MS,
Perfluorobutanesulfonic acid (PFBuS),
Perfluorodecanoic acid (PFDA),
Perfluoroheptanoic acid (PFHpA),
Perfluorohexanesulfonic acid (PFHxS),
Perfluorohexanoic acid (PFHxA),
Perfluorononanoic acid (PFNA),
Perfluorooctanesulfonic acid (PFOS),
Perfluorooctanoic acid (PFOA),
Persistant organic pollutants (POP),
Polyfluorinated acids,
Water
This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL.
resin, polymer, EVOLUTE,
Tags: Application Notes,
Biomarker,
Clinical,
Column,
Diethyldithiophosphate (DEDTP),
Diethylphosphate (DEP),
Diethylthiophosphate (DETP),
Dimethyldithiophosphate (DMDTP),
Dimethylphosphate (DMP),
Dimethylthiophosphate (DMTP),
LC-MS/MS,
Organophosphate Pesticide,
Urine
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Tags: Alcohol metabolite,
Application Notes,
Biomarker,
Brochures,
Catecholamines,
Clinical,
English,
Pain Management,
Peptides,
Product Notes,
Steroid Hormones,
Steroid metabolite,
Thyroid Hormone,
Tobacco Specific Nitrosamines (TSNAs),
Vitamin D,
Vitamins,
Water Soluble Vitamins
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags: Catalogs
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate,
Clinical,
Column,
English,
Environmental,
Food,
Forensic,
Manuals & User Guides,
On-line SPE,
Pharma
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
This poster describes an automated method for solid phase extraction of 62 PFAS compounds from drinking and environmental waters using Biotage Extrahera and EVOLUTE EXPRESS WAX SPE columns.
ASMS 2019
Tags: Column,
English,
Environmental,
Extrahera,
LC-MS/MS,
PFAA,
PFAAs,
Perfluorobutanesulfonic acid (PFBuS),
Perfluorodecanoic acid (PFDA),
Perfluoroheptanoic acid (PFHpA),
Perfluorohexanesulfonic acid (PFHxS),
Perfluorohexanoic acid (PFHxA),
Perfluorononanoic acid (PFNA),
Perfluorooctanesulfonic acid (PFOS),
Perfluorooctanoic acid (PFOA),
Posters
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
ASMS 2017
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH),
6-acetylmorphine (MAM),
7-Aminoclonazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Buprenorphine,
Butalbital,
Cannabinoid,
Carbamate hypnotic,
Carbamate muscle relaxant,
Carisoprodol,
Chlordiazepoxide,
Clinical,
Clonazepam,
Cocaine,
Cocaines,
Codeine,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Morphine,
N-des-tapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Nortryptiline,
Opiates,
Opioid,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Phencyclidine,
Phenobarbital,
Posters,
Pregabalin,
Ritalinic acid,
Secobarbital,
Tapentadol,
Temazepam,
Tramadol,
Urine,
Zolpidem,
Zolpidem-phenyl-4-COOH,
z-drugs,
α-OH alprazolam
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
ASMS 2017
Tags: 96-well plate,
Clinical,
English,
Oxytocin,
Peptides,
Pharma,
Posters,
Serum,
Vasopressin
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
ASMS 2017
Tags: 96-well plate,
Amikacin,
Aminoglycoside antibiotics,
Apramycin,
Dihydrostreptomycin,
Eggs,
English,
Extrahera,
Food,
Gentamicin,
Gentamicin C1,
Gentamicin C1a,
Gentamicin C2,
Hygromycin B,
Kanamycin,
LC-MS/MS,
Meat,
Milk,
Neomycin,
Netilmicin,
Paromomycin,
Posters,
Sisomycin,
Spectinomycin,
Streptomycin,
Tobramycin,
Veterinary drugs
This poster summarizes various sample preparation strategies for the
extraction of MMA from serum without the necessity for derivatization, prior to LC-MS/MS analysis. A range of sample preparation techniques of varying complexity were evaluated: protein precipitation, phospholipid depletion, supported liquid extraction and solid phase extraction using both silica and polymer-based mixed-mode anion exchange chemistries.
Method performance was evaluated for evaporative effects, assay recovery, ion suppression and phospholipid removal.
MSACL 2016
Tags: 96-well plate,
Acidic,
Clinical,
English,
LC-MS/MS,
MMA (Methylmalonic acid),
Methylmalonic acid (MMA),
Posters,
Serum
EVOLUTE® EXPRESS SPE columns and plates combine sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning; thus simplifying and reducing extraction processing. This poster compares the performance of various EVOLUTE EXPRESS column chemistries using 10-500 mg bed weights, in order to investigate whether sorbent conditioning and equilibration steps are truly required.
ASMS 2015
Tags: Acidic,
Amitriptyline,
Atenolol,
Benzenesulfonic acid,
Bretylium,
Clinical,
Column,
Diethyldithiophosphate (DEDTP),
Dimethylthiophosphate (DMTP),
English,
Extrahera,
Forensic,
Hexanesulfonic acid,
Ketoprofen,
LC-MS/MS,
Metoprolol,
Mianserin,
Organophosphate Pesticide,
Over the counter pharmaceuticals,
Pain Management,
Pharma,
Pharmaceuticals,
Posters,
Prednisolone,
Procainamide,
Quaternary Amines,
Quinidine,
Salbutamol,
Sulindac,
Tricyclic Antidepressant,
Urine,
β-blockers
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
HPLC 2014
Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
HPLC2014
Tags: Biological fluids,
English,
HPLC,
LC-MS/MS,
Plasma,
Posters
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Pittcon 2014
Tags: 96-well plate,
ACTH (1-24),
Angiotensin Fragment 1-7,
Angiotensin II,,
Clinical,
Elafin,
Endorphin,
English,
Glycoprotein myelin,
Helicobacter pylori (2-20),
LC-MS/MS,
Peptides,
Pharma,
Plasma,
Posters,
Serum,
Vasoactive Intestinal Fragment 1-12
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24),
Alpha-Endorphin,
Angiotensin Fragment 1-7,
Angiotensin II,,
Elafin,
Helicobacter pylori (2-20),
LC-MS/MS,
Myelin Glycoprotein fragment (35-55) rat,
Peptides,
Pharma,
Plasma,
Posters,
Vasoactive Intestinal Fragment 1-12
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Tags: 96-well plate,
Alcohol metabolite,
Column,
Ethyl Sulfate (EtS),
Ethyl glucuronide (EtG),
Forensic,
LC-MS/MS,
Posters,
Urine
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Tags: Alcohol metabolite,
Application Notes,
Biomarker,
Brochures,
Catecholamines,
Clinical,
English,
Pain Management,
Peptides,
Product Notes,
Steroid Hormones,
Steroid metabolite,
Thyroid Hormone,
Tobacco Specific Nitrosamines (TSNAs),
Vitamin D,
Vitamins,
Water Soluble Vitamins
This video is a quick run-through of Application AN883 - Extraction of Polyfluorinated Compounds in Water Using EVOLUTE® EXPRESS WAX SPE Prior to LC-MS/MS Analysis. Watch all...
14 September 2018Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldo...
13 August 2018Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
24 October 2017This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...
05 July 2017This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...
29 May 2017Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...
19 May 2017The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...
20 April 2017Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...
06 December 2016Could there be a sorbent for versatile sample preparation that doesn't need conditioning and equilibration? Actually, Biotage has developed five such sorbents in the EVOLUTE® EXPRESS family, an...
02 February 2016Five new application notes from Biotage labs provide a suite of methods for effective extraction of methylmalonic acid from serum using modern sample preparation techniques and materials from ...
15 October 2015A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...
27 March 2015Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
25 March 2015This application note demonstrates the use of EVOLUTE® WAX solid phase extraction columns for the extraction of a range of polyfluorinated (PFA) compounds from water samples. The ...
29 July 2014Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
10 July 2014Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...
30 June 2014Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/
20 May 2014Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...
01 April 2014Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...
11 January 2014Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...
28 August 2012Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...
28 August 2012EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...
18 April 2011Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...
16 December 2010Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...
23 February 2010Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Question:
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
Answer:
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
Browse more questions and answers >EVOLUTE® WAX combines non-polar and weak anion exchange functionality for extraction of strongly acidic analytes from biological fluids and other aqueous samples through a dual, mixed-mode mechanism. This provides additional selectivity and clean-up for complex samples, with salts, phospholipids and proteins eliminated from extracts. Available in a variety of formats, including modular and 96-well plates and SPE columns, and two different particle sizes (30 µm and 50 µm) depending on application requirements.
The sorbent consists of the EVOLUTE backbone, surface modified with primary/secondary amine groups.
Figure 1. Structure of EVOLUTE® WAX sorbent
EVOLUTE® WAX sorbent is available in EVOLUTE® EXPRESS SPE columns and 96-well plate formats for use with fast Load-Wash-Elute procedures. Eliminate traditional conditioning and equilibration and increase throughput without compromising performance.
EVOLUTE sorbents have a narrow pore diameter, tailored to eliminate co-extraction of proteins from biofluids. Removal of proteins during sample preparation avoids transfer in to the analytical system, reducing matrix effects in LC-MS/MS, and avoiding contamination of the analytical column. This minimises the need for frequent backflushing and guard columns replacement.
Using EVOLUTE WAX, interferences including proteins, salts, non-ionisable molecules and phospholipids are removed during the interference elution step, ensuring extremely clean extracts (figure 2).
Figure 2. The combination of optimized pore structure and additional interference elution steps using mixed-mode sorbent chemistry guarantees extremely clean extracts.
EVOLUTE® WAX uses a generic procedure for extraction of strongly acidic analytes in aqueous matrices. The dual retention mechanism retains strong acids through a combination of non-polar and weak anion exchange interactions. The simple wash steps in the generic method remove >98% of phospholipids from plasma samples.
The combination of robust polymer chemistry, uniform coverage of secondary amine groups and optimum exchange capacity provide greater than 85 % recovery for a wide range of strongly acidic analytes.
See the EVOLUTE User Guide for a wealth of method optimisation information.
Figure 2. Recovery of strongly acidic analytes from urine samples using EVOLUTE WAX fixed well plate. Analytes: dimethylthiophosphate (DMTP), diethylphosphate (DEP), hexanesulfonic acid (HSA), benzenesulfonic acid (BSA).
Narrow particle size distribution provides consistent flow characteristics for a variety of sample matrices. Optimized packing techniques eliminate channelling, which can lead to analyte breakthrough.
Even viscous biofluids can be loaded onto EVOLUTE EXPRESS WAX products without the need for conditioning and equilibration using fast load-wash-elute SPE.
The surface characteristics of each batch of EVOLUTE® WAX are quality controlled and tested to ensure consistency.
Part No. | Description | Product type | Pack Size | Price | ||
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PPM-A96-CH | 1 mL (Tabless) Column Holder, Biotage® PRESSURE+ 96 | Accessories | 1 | Log in for price |